Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures

Gnilitskyi, Iaroslav; Pogorielov, Мaksym; Viter, Roman; Ferraria, Ana Maria; Carapeto, Ana P.; Oleshko, Oleksandr; Orazi, Leonardo; Mishchenko, Oleg

doi:10.1016/j.nano.2019.102036

Cited by 51 publications

(41 citation statements)

References 38 publications

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“…Besides the topographical and chemical characteristics of the Zirconia biointerfaces, another issue is represented by the lack of knowledge of a more realistic model for in vitro biotesting. Until now, the in vitro studies on the biological response to Zirconia entailed mainly investigating the activity of osteoblast and osteoblast-like cells, fibroblasts, and epithelial cell lines [41,42]. There are few studies related to the interaction of MSCs with micro-nanotopographies generated in Zirconia-based materials, treating mainly pit-like or ditch-like topographies.…”

Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

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Osseous implantology’s material requirements include a lack of potential for inducing allergic disorders and providing both functional and esthetic features for the patient’s benefit. Despite being bioinert, Zirconia ceramics have become a candidate of interest to be used as an alternative to titanium dental and cochlear bone-anchored hearing aid (BAHA) implants, implying the need for endowing the surface with biologically instructive properties by changing basic parameters such as surface texture. Within this context, we propose anisotropic and isotropic patterns (linear microgroove arrays, and superimposed crossline microgroove arrays, respectively) textured in zirconia substrates, as bioinstructive interfaces to guide the cytoskeletal organization of human mesenchymal stem cells (hMSCs). The designed textured micro-nano interfaces with either steep ridges and microgratings or curved edges, and nanoroughened walls obtained by direct femtosecond laser texturing are used to evaluate the hMSC response parameters and osteogenic differentiation to each topography. Our results show parallel micro line anisotropic surfaces are able to guide cell growth only for the steep surfaces, while the curved ones reduce the initial response and show the lowest osteogenic response. An improved osteogenic phenotype of hMSCs is obtained when grown onto isotropic grid/pillar-like patterns, showing an improved cell coverage and Ca/P ratio, with direct implications for BAHA prosthetic development, or other future applications in regenerating bone defects.

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Section: Introductionmentioning

confidence: 99%

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Sima

Bonciu

Baciu³

et al. 2020

Nanomaterials

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“…Further, in paper Low Frequency LIPSS will be noted like LIPSS, while High Frequence LIPSS will be indicated as it is. LIPSS are already used and have a potential to be applied in numerous fields, including improving adhesion [5], wettability [6], surface colorization [7], better proliferation and adhesion of tissue cells [8], and others.…”

Section: Introductionmentioning

confidence: 99%

Tribological Properties of High-Speed Uniform Femtosecond Laser Patterning on Stainless Steel

et al. 2019

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In this work, an analysis of the tribological performance of laser-induced periodic surface structures (LIPSS) treated X5CrNi1810 stainless steel was conducted. The approach followed by authors was to generate LIPSS-patterned circular tracks, composed of radial straight grooves with uniform angular periodicity. This permitted to measure the tribological properties in a pin-on-flat configuration, keeping fixed the orientation between the grooves and the sliding direction. A Stribeck curve was measured, as well as the consequent wear. A deep analysis of the sub-surface conditions after LIPSS generation was moreover performed using Focused Ion Beam (FIB) cross-section.

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“…An appropriate or relatively higher scanning line overlap leads to a homogenous distribution of nanostructures and helps avoid strong waviness of the laser treated surface. Several studies have already shown that laser induced periodic surface structures (FLIPSS, LIPSS) provide favorable conditions for cell attachment [15,17,58,59]. The impact of two selected micro and two selected FLIPSS structures presented in the current study on the cell adhesion of MG-63 cells have already been investigated [15].…”

Section: It Can Be Deduced Frommentioning

confidence: 75%

“…Laser treatment of titanium has been found to be particularly advantageous compared to conventional surface structuring methods because it leads to less contamination of the surfaces [12] and offers the possibility of creating stochastic as well as precise deterministic structures in the microand nanometer range [5,13,14]. In particular, the potential of femtosecond (fs) laser structuring of titanium for use in biomedical implants has been shown in several studies [15][16][17][18]. This technique is superior to nanosecond laser treatments due to opportunity of creating surface topographies with a greater variety of patterns [19].…”

Section: Introductionmentioning

confidence: 99%

Effect of Laser Pulse Overlap and Scanning Line Overlap on Femtosecond Laser-Structured Ti6Al4V Surfaces

2020

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Surface structuring is a key factor for the tailoring of proper cell attachment and the improvement of the bone-implant interface anchorage. Femtosecond laser machining is especially suited to the structuring of implants due to the possibility of creating surfaces with a wide variety of nano- and microstructures. To achieve a desired surface topography, different laser structuring parameters can be adjusted. The scanning strategy, or rather the laser pulse overlap and scanning line overlap, affect the surface topography in an essential way, which is demonstrated in this study. Ti6Al4V samples were structured using a 300 fs laser source with a wavelength of 1030 nm. Laser pulse overlap and scanning line overlap were varied between 40% and 90% over a wide range of fluences (F from 0.49 to 12.28 J/cm²), respectively. Four different main types of surface structures were obtained depending on the applied laser parameters: femtosecond laser-induced periodic surface structures (FLIPSS), micrometric ripples (MR), micro-craters, and pillared microstructures. It could also be demonstrated that the exceedance of the strong ablation threshold of Ti6Al4V strongly depends on the scanning strategy. The formation of microstructures can be achieved at lower levels of laser pulse overlap compared to the corresponding value of scanning line overlap due to higher heat accumulation in the irradiated area during laser machining.

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Cell and tissue response to nanotextured Ti6Al4V and Zr implants using high-speed femtosecond laser-induced periodic surface structures

Cited by 51 publications

References 38 publications

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Bioinstructive Micro-Nanotextured Zirconia Ceramic Interfaces for Guiding and Stimulating an Osteogenic Response In Vitro

Tribological Properties of High-Speed Uniform Femtosecond Laser Patterning on Stainless Steel

Effect of Laser Pulse Overlap and Scanning Line Overlap on Femtosecond Laser-Structured Ti6Al4V Surfaces

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