Cell and tissue level responses in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated Ag nanoparticles at two seasons

Duroudier, Nerea; Katsumiti, Alberto; Mikolaczyk, Mathilde; Schäfer, Jörg; Bilbao, Eider; Cajaraville, Miren P.

doi:10.1016/j.scitotenv.2020.141303

Cited by 5 publications

(6 citation statements)

References 83 publications

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“…The copyright holder for this preprint (which this version posted July 20, 2021. ; https://doi.org/10.1101/2021.07.19.452197 doi: bioRxiv preprint associated to sulphur (George et al, 1986) whilst AgNP -in particular larger ones-are more readily available to the digestive tissues (this work; Gomes et al, 2014;Duroudier et al, 2019;Duroudier et al, 2021). Overall, this body of evidence may explain the existence of a double contribution to apical toxicity of AgNP -i.e.…”

Section: Discussionmentioning

confidence: 71%

“…Our data on silver uptake dynamics in gills and digestive gland after 96 h exposure indicated the former route is almost exclusive for ionic silver (95%), dominant for 5 nm AgNP (85%) and even for larger 50 nm AgNP (40%) (Figure 7). It should be said that in long-term exposure to ionic silver, in contrast to other heavy metals, silver did not accumulate in the digestive tubules within lysosomes but in the basement membranes and tissue macrophages in some forms associated to sulphur (George et al, 1986) whilst AgNP -in particular larger ones-are more readily available to the digestive tissues (this work; Gomes et al, 2014;Duroudier et al, 2019;Duroudier et al, 2021). Overall, this body of evidence may explain the existence of a double contribution to apical toxicity of AgNP -i.e.…”

Section: Discussionmentioning

confidence: 84%

“…The copyright holder for this preprint (which this version posted July 20, 2021. ; https://doi.org/10.1101/2021.07.19.452197 doi: bioRxiv preprint dietary exposure is a much better vehicle for silver bioaccumulation (Duroudier et al, 2019;Duroudier et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…They could, however, highlight protein-associated metal deposits in the digestive gland (but not gills, mantle and gonads) by means of auto-metallography. The same research group claimed that dietary exposure is a much better vehicle for silver bioaccumulation (Duroudier et al, 2019;Duroudier et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Ecotoxicological effects of silver nanoparticles in marine mussels

Calisi

Lorusso

Gallego-Urrea

et al. 2021

Preprint

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In the marine bioindicator species M. galloprovincialis Lam we predicted toxicity and bioaccumulation of 5 nm alkane-coated and 50 nm uncoated silver nanoparticles (AgNPs) along with Ag+, as a function of the actual dose level. We generated a time persistence model of silver concentration in seawater and used the Area Under the Curve (AUC) as independent variable in hazard assessment. This approach allowed us to evaluate unbiased ecotoxicological endpoints for acute (survival) and chronic toxicity (byssal adhesion). Logistic regression analysis rendered LC5096h values of 0.68 ± 0.08; 1.00 ± 0.20; 1.00 ± 0.42 mg h L-1 respectively for Ag+, 5 nm and 50 nm AgNP posing no evidence the silver form is a necessary variable to predict the survival outcome. By contrast, for byssal adhesion regression analysis revealed a much higher toxicological potential of Ag+ vs AgNPs, 0.0021 ± 0.0009; 0.053 ± 0.016; 0.021 (no computable error for 50 nm AgNP) mg h L-1, and undoubtedly confirmed a role of the silver form. Bioaccumulation was higher for Ag+ > 5 nm AgNP > 50 nm AgNP reflecting a parallel with the preferential uptake route / target organ. We, eventually, provided a full range of toxicological endpoints to derive risk quotients.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Ecotoxicological effects of silver nanoparticles in marine mussels

Calisi

Lorusso

Gallego-Urrea

et al. 2021

Preprint

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“…The Single Cell Gel Electrophoresis assay (SCGE), known as the Comet assay was used to assess the DNA strand breaks according to Duroudier et al (2021). The analysis of DNA damage was performed using OPTIKA B-350 fluorescent microscope (OPTIKA, Ponteranica, Italy), with a CCD camera.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Silver Nanoparticles Genotoxicity in Hermetia illucens Using Comet Assay

Abdelfattah

2022

ije

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Silver nanoparticles (AgNPs) are commonly used in various sectors such as food, cosmetics, medicine, and insect control. Otherwise, the toxicological effects of this promising technology should be studied to ensure its safety. This research aimed to investigate the potential toxicity of different concentration of AgNPs (0-20 mg/mL) on the cuticle cells of insects using alkaline comet assay. The level of DNA damaged was significantly higher in insects treated with 5-20 mg/mL AgNPs comparing to that from the control one with the fold of 1, 1.7, 1.9, 4.3, respectively in the tail length. A strong positive correlation occurred between concentration of AgNPs and all comet assay parameters were occurred with linear prediction equations. The possible deleterious impacts of AgNPs on the Hermetia illucens (L.) were discussed. Also, the potential using of comet assay as an accurate and cost-effective monitoring tool of fate of using nanoparticles was proposed.

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An Updated Review on Ag NP Effects at Organismal Level: Internalization, Responses, and Influencing Factors

et al. 2022

Reviews Env.Contamination (formerly:Residue Reviews)

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Cell and tissue level responses in mussels Mytilus galloprovincialis dietarily exposed to PVP/PEI coated Ag nanoparticles at two seasons

Cited by 5 publications

References 83 publications

Ecotoxicological effects of silver nanoparticles in marine mussels

Ecotoxicological effects of silver nanoparticles in marine mussels

Evaluation of Silver Nanoparticles Genotoxicity in Hermetia illucens Using Comet Assay

An Updated Review on Ag NP Effects at Organismal Level: Internalization, Responses, and Influencing Factors

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