Cell and tissue behavior on micro-grooved surfaces

Walboomers, X. Frank; Jansen, John A.

doi:10.1007/s10266-001-8178-z

Cited by 106 publications

(73 citation statements)

References 67 publications

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“…In addition, the observation of multilayered cells in the multigrooves indicates the production of a large quantity of ECM. The results of this study are supported from the in vivo examinations by Brunette et al 5,23,28 and Walboomers et al 7,22 Brunette et al placed epoxy implants with V-shaped grooves having depths of 22, 10, or 3 m into rat skin and found that the deeper grooved surfaces inhibited epithelial downgrowth. Walboomers et al investigated the in vivo behavior of microgrooved implants in soft tissue of the goat.…”

Section: Discussionsupporting

confidence: 88%

Effects of multigrooved surfaces on fibroblast behavior

Yoshinari

Matsuzaka

Inoue

et al. 2003

J Biomedical Materials Res

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Microgrooves have been investigated as substrates for the control of cell alignment. However, they are relatively too narrow and shallow for controlling the orientation of extracellular matrices (ECM) such as collagen. Multigrooves, a combination of microgrooves and macrogrooves, are expected to be able to control the orientation of both cells and ECM. This study investigated a method for fabricating multigrooves and evaluated fibroblast behavior on these novel surfaces. Multigrooved patterns were fabricated on a gold-alloy metal die, in which 90-degree V-shaped microgrooves with a 2-microm pitch were cut on trapezoidal macrogrooves. The macrogrooves had a 50- microm ridge width, a 50-microm wall width, a 50-microm bottom width, and a 25-microm depth. The grooves were made by an ultraprecision micromachine using a single crystal diamond. This metal die served as a template for making surface replicas from polystyrene. Microgrooved and smooth polystyrene replicas also were prepared as comparative substrates. Mouse fibroblast L929 cells were cultured in each type of replica substrate for 7 to 21 days. After these periods, the cells were fixed with 2.5% glutaraldehyde, treated with conventional methods, and, finally, observed by SEM. Confocal laser scanning microscopy was performed to investigate ECM formation. The multigrooved metal die exhibited the desired sharp configuration without defects. The dimensional values of the multigrooves on the polystyrene replicas were almost the same as the designed values. The fibroblasts on the multigrooved and microgrooved substrates were aligned parallel to the surface grooves after 7 days of incubation. In contrast to the microgrooved and flat surfaces, a dense extracellular matrix was produced along the multigrooves after 21 days of incubation. These results suggest that multigrooves can control the orientation of ECM as well as cells and thus enhance the production of ECM.

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Section: Discussionsupporting

confidence: 88%

Effects of multigrooved surfaces on fibroblast behavior

Yoshinari

Matsuzaka

Inoue

et al. 2003

J Biomedical Materials Res

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“…Anisotropic surfaces such as grooved substrata can control cell shape, orientation, division, and the direction of cell migration [Brunette, 1986a;Clark et al, 1990;Oakley and Brunette, 1993;Oakley et al, 1997;Walboomers et al, 1999Walboomers et al, , 2000Walboomers and Jansen, 2001;Dalby et al, 2003;Hamilton et al, 2005a;. This phenomenon is termed topographic or contact guidance [Dunn and Heath, 1976;Brunette, 1986a;Clark et al, 1987Clark et al, , 1990Curtis and Wilkinson, 1998] and is exhibited by a variety of cell populations contacting grooved substrata of various chemical compositions, fabricated by different techniques and having different groove/ridge geometries and dimensions [Brunette et al, 1983;Chehroudi et al, 1988;Clark et al, 1991;den Braber et al, 1995den Braber et al, , 1996Walboomers et al, 1998;Miller et al, 2001;Dalby et al, 2003;Curtis et al, 2005;Evans et al, 2005].…”

Section: Introductionmentioning

confidence: 98%

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Hamilton

Oakley

Jaeger

et al. 2009

Cell Motil. Cytoskeleton

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Anisotropic substrata such as micromachined grooves can control cell shape, orientation, and the direction of cell movement, a phenomena termed topographic guidance. Although many types of cells exhibit topographic guidance, little is known regarding cell responses to conflicting topographic cues. We employed a substratum with intersecting grooves in order to present fibroblasts and epithelial cells with conflicting topographic cues. Using time-lapse and confocal microscopy, we examined cell behavior at groove intersections. Migrating fibroblasts and epithelial cells typically extended a cell process into the intersection ahead of the cell body. After travelling along the "X" groove to enter the intersection, the leading lamellipodia of the cell body encountered the perpendicular "Y" groove, and spread latterly along the "Y" groove. The formation of lateral lamellipodia resulted in cells forming "T" or "L" morphologies, which were characterized by the formation of phosphotyrosine-rich focal adhesions at the leading edges. The "Y" groove did not prove an absolute barrier to cell migration, particularly for epithelial cells. Analysis of cytoskeletal distribution revealed that F-actin bundles did not adapt closely to the groove patterns, but typically did align to either the "X" or "Y" grooves. In contrast microtubules (MT) adapted closely to the walls. Inhibition of microtubule nucleation attenuated fibroblast and epithelial cell orientation within the intersection of the perpendicular grooves. We conclude that MT may be the prime determinant of fibroblast and epithelial cell conformation to conflicting topographies.

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“…In the leading edge, new cell adhesions are formed, and the actin meshwork contracts, which causes the cell to move in the direction of the new adhesion [Walboomers and Jansen, 2001]. In our study, the surface structures consist of Ti oxide lines with varied width and distance but a common height of only 12 nm.…”

Section: Surface Structure and Cell Orientation: Mechanical Guidance?mentioning

confidence: 99%

“…According to Walboomers and Jansen [2001], precursor contacts precede the formation of a focal contact and are made in two steps: (i) a nonspecifi c approach of the cell membrane to the substrate surface based on electrostatic or much weaker van der Waals forces, and (ii) the specifi c binding via membrane integrin receptors to ECM proteins absorbed on the implant surface. At the interface between Ti ground and the Ti oxide lines, the surface charge density may differ due to varying physicochemical properties of the thin naturally formed oxide fi lm and the thicker thermal-induced oxide lines.…”

Section: Surface Structure and Cell Orientation: Guidance By Surface mentioning

confidence: 99%

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

Monsees¹,

Barth²,

Tippelt³

et al. 2005

Cells Tissues Organs

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To test nanosize surface patterning for application as implant material, a suitable titanium composition has to be found first. Therefore we investigated the effect of surface chemistry on attachment and differentiation of osteoblast-like cells on pure titanium prepared by pulsed laser deposition (TiPLD) and different Ti alloys (Ti6Al4V, TiNb30 and TiNb13Zr13). Early attachment (30 min) and alkaline phosphatase (ALP) activity (day 5) was found to be fastest and highest, respectively, in cells grown on TiPLD and Ti6Al4V. Osteoblasts seeded on TiPLD produced most osteopontin (day 10), whereas expression of this extracellular matrix protein was an order of magnitude lower on the TiNb30 surface. In contrast, expression of the corresponding receptor, CD44, was not influenced by surface chemistry. Thus, TiPLD was used for further experiments to explore the influence of surface nanostructures on osteoblast adhesion, differentiation and orientation. By laser-induced oxidation, we produced patterns of parallel Ti oxide lines with different widths (0.2–10 µm) and distances (2–20 and 1,000 µm), but a common height of only 12 nm. These structures did not influence ALP activity (days 5–9), but had a positive effect on cell alignment. Two days after plating, the majority of the focal contacts were placed on the oxide lines. The portion of larger focal adhesions bridging two lines was inversely related to the line distance (2–20 µm). In contrast, the portion of aligned cells did not depend on the line distance. On average, 43% of the cells orientated parallel towards the lines, whereas 34% orientated vertically. In the control pattern (1,000 µm line distance), cell distribution was completely at random. Because a significant surplus of the cells preferred a parallel alignment, the nanosize difference in height between Ti surface and oxide lines may be sufficient to orientate the cells by contact guiding. However, gradients in electrostatic potential and surface charge density at the Ti/Ti oxide interface may additionally influence focal contact formation and cell guidance.

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Cell and tissue behavior on micro-grooved surfaces

Cited by 106 publications

References 67 publications

Effects of multigrooved surfaces on fibroblast behavior

Effects of multigrooved surfaces on fibroblast behavior

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Effects of Different Titanium Alloys and Nanosize Surface Patterning on Adhesion, Differentiation, and Orientation of Osteoblast-Like Cells

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