Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gold surfaces

Lindblad, Marianne

doi:10.1016/s0142-9612(97)00029-x

Cited by 70 publications

(59 citation statements)

References 43 publications

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“…This assertion that methyl-terminated, alkyl surfaces can be excellent substrates for human cell culture is unique among the published reports of cellular interactions with alkyl monolayers. 27,29,[31][32][33]35,36 Our findings are not surprising, however, because as motile phagocytes, macrophages must be able to adhere to a wide range of foreign materials and reside in a variety of human tissues.…”

Section: Discussionmentioning

confidence: 65%

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Jenney

Anderson

1999

J. Biomed. Mater. Res.

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A homologous set of alkylsilane-modified glass surfaces with chain lengths ranging from methyl to octadecyl was prepared in order to examine the influence of alkyl surface chemistry on macrophage adhesion and foreign body giant cell (FBGC) formation. Contact angle and X-ray photoelectron spectroscopy analysis confirmed our silanation technique and indicated a consistent alkyl chain density independent of chain length. Human peripheral blood monocytes were isolated and cultured on these alkylsilane surfaces for a period of 10 days. The initial density of human monocytes was similar on all surfaces. Beyond day 0 the clean glass, methyl (DM and C1), propyl (C3), and hexyl (C6) surfaces maintained a high cell density and supported macrophage development. In contrast, long-term macrophage density was extremely low on the tetradecyl (C14) and octadecyl (C18) surfaces. When interleukin-4 was added to induce FBGC formation in vitro, the DM, C1, C3, and C6 surfaces supported high levels of macrophage fusion while clean glass strongly inhibited fusion. The C14 and C18 surfaces did not contain sufficient macrophages to support FBGC formation. Cage implant studies revealed that in vivo macrophage density and FBGC formation on clean glass and C6 surfaces was similar to in vitro data. In contrast to the monocyte culture results, the C18 cage implant samples supported significant FBGC formation, possibly as a result of different conditions within each experimental system. Radiotracer adsorption studies of eight human serum proteins identified the high concentration and tenacious hold of adsorbed von Willebrand factor as being possibly involved in the poor long-term macrophage density observed on C14 and C18.

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Section: Discussionmentioning

confidence: 65%

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Jenney

Anderson

1999

J. Biomed. Mater. Res.

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“…Hydrophobic surfaces support the adsorption of various surfactants and proteins from water because expulsion of solute from solution into the interphase between the bulk solid and solution phases is energetically favorable. Adsorption to hydrophobic surfaces is driven by the reduction of interfacial energetics concomitant with the replacement of water molecules at the surface by the adsorbed solute (surface dehydration) (21,22). In contrast, it was reported that hydrophilic surfaces with a sulfonate group lead to greater adsorption of proteins from plasma (6), and that plasma protein adsorption on a wettability gradient surface is elevated with an increase in surface wettability.…”

Section: Discussionmentioning

confidence: 99%

Oxygen plasma surface modification enhances immobilization of simvastatin acid

et al. 2006

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Simvastatin acid (SVA) has been reported to stimulate bone formation with increased expression of BMP-2. Therefore, immobilization of SVA onto dental implants is expected to promote osteogenesis at the bone tissue/implant interface. The aim of this study was to evaluate the immobilization behavior of SVA onto titanium (Ti), O 2 -plasma treated titanium (Ti + O2), thin-film coatings of hexamethyldisiloxane (HMDSO), and O 2 -plasma treated HMDSO (HMDSO + O2) by using the quartz crystal microbalance-dissipation (QCM-D) technique. HMDSO surfaces were activated by the introduction of an OH group and/or O 2 -functional groups by O 2 -plasma treatment. In contrast, titanium surfaces showed no appreciable compositional changes by O 2 -plasma treatment. The QCM-D technique enabled evaluation even at the adsorption behavior of a substance with a low molecular weight such as simvastatin. The largest amount of SVA was adsorbed on O 2 -plasma treated HMDSO surfaces compared to untreated titanium, HMDSO-coated titanium, and O 2 -plasma treated titanium. These findings suggested that the adsorption of SVA was enhanced on more hydrophilic surfaces concomitant with the presence of an OH group and/or O 2 -functional group resulting from the O 2 -plasma treatment, and that an organic film of HMDSO followed by O 2 -plasma treatment is a promising method for the adsorption of SVA in dental implant systems.Simvastatin, one of the liposoluble statins [inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A (HMGCoA) reductase], has been widely used for lowering cholesterol levels. Recently, simvastatin was reported to stimulate bone formation both in vitro and in vivo in animal models of osteoporosis (10,25). This phenomenon was associated with increased expression of the bone morphogenetic protein-2 (BMP-2) gene in bone cells. Therefore, immobilization of simvastatin onto titanium implants is expected to promote osteogenesis in the bone tissue surrounding the implants through its topical application. Furthermore, one distinct advantage of simvastatin for local stimulation of bone formation is its low manufacturing cost compared to the direct administration of recombinant proteins such as BMPs. There is also a reduced possibility of eliciting antibody responses.Plasma-surface modification including plasma polymerization and plasma surface treatment is an effective and economical surface treatment technique for many materials. The technique is of growing interest in biomedical engineering because it can control the physicochemical properties of biomaterials

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“…The oxidative response and secretion of molecules are influenced by both the properties of the material and the component of their microenvironment. [43][44][45] The early involvement of these cells plays an important role in the inflammatory events and the repair and/or regeneration of soft tissues and bone in association with the implant. 46,47 In addition, when these implant-tissue interface cells encounter and interact with live bacteria, components of their cell walls, and other molecules, it is of paramount importance that an efficient immune response is elicited.…”

Section: Monocyte Activation In Response To Different Substrates and mentioning

confidence: 99%

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

Svensson¹,

Forsberg²,

Hulander³

et al. 2014

IJN

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The role of material surface properties in the direct interaction with bacteria and the indirect route via host defense cells is not fully understood. Recently, it was suggested that nanostructured implant surfaces possess antimicrobial properties. In the current study, the adhesion and biofilm formation of Staphylococcus epidermidis and human monocyte adhesion and activation were studied separately and in coculture in different in vitro models using smooth gold and well-defined nanostructured gold surfaces. Two polystyrene surfaces were used as controls in the monocyte experiments. Fluorescent viability staining demonstrated a reduction in the viability of S. epidermidis close to the nanostructured gold surface, whereas the smooth gold correlated with more live biofilm. The results were supported by scanning electron microscopy observations, showing higher biofilm tower formations and more mature biofilms on smooth gold compared with nanostructured gold. Unstimulated monocytes on the different substrates demonstrated low activation, reduced gene expression of pro- and anti-inflammatory cytokines, and low cytokine secretion. In contrast, stimulation with opsonized zymosan or opsonized live S. epidermidis for 1 hour significantly increased the production of reactive oxygen species, the gene expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, and IL-10, as well as the secretion of TNF-α, demonstrating the ability of the cells to elicit a response and actively phagocytose prey. In addition, cells cultured on the smooth gold and the nanostructured gold displayed a different adhesion pattern and a more rapid oxidative burst than those cultured on polystyrene upon stimulation. We conclude that S. epidermidis decreased its viability initially when adhering to nanostructured surfaces compared with smooth gold surfaces, especially in the bacterial cell layers closest to the surface. In contrast, material surface properties neither strongly promoted nor attenuated the activity of monocytes when exposed to zymosan particles or S. epidermidis .

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Cell and soft tissue interactions with methyl- and hydroxyl-terminated alkane thiols on gold surfaces

Cited by 70 publications

References 43 publications

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Alkylsilane-modified surfaces: Inhibition of human macrophage adhesion and foreign body giant cell formation

Oxygen plasma surface modification enhances immobilization of simvastatin acid

Role of nanostructured gold surfaces on monocyte activation and Staphylococcus epidermidis biofilm formation

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