Cell and nuclear degradation in root meristems following exposure of potatoes (Solanum tuberosum L.) to salinity

Richardson, Kim; Wetten, A.; Caligari, P. D. S.

doi:10.1007/bf02358598

Cited by 13 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Salt stress causes nuclear and DNA degradation in root meristematic cells (Katsuhara and Kawasaki, 1996 ; Liu et al, 2000 ; Richardson et al, 2001 ), and NaCl-induced nuclear DNA fragmentation in the root meristem zone was higher in sos1 mutant (lacking the ability to remove Na + to external media) compared with Arabidopsis wild type, when grown under saline conditions (Huh et al, 2002 ). From this point of view, one would expect that salt-tolerant varieties would maintain lower cytosolic Na + intensity in meristem cells.…”

Section: Discussionmentioning

confidence: 99%

Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots

Shabala

Liu

et al. 2015

Front. Plant Sci.

View full text Add to dashboard Cite

Salinity stress tolerance is a physiologically complex trait that is conferred by the large array of interacting mechanisms. Among these, vacuolar Na+ sequestration has always been considered as one of the key components differentiating between sensitive and tolerant species and genotypes. However, vacuolar Na+ sequestration has been rarely considered in the context of the tissue-specific expression and regulation of appropriate transporters contributing to Na+ removal from the cytosol. In this work, six bread wheat varieties contrasting in their salinity tolerance (three tolerant and three sensitive) were used to understand the essentiality of vacuolar Na+ sequestration between functionally different root tissues, and link it with the overall salinity stress tolerance in this species. Roots of 4-day old wheat seedlings were treated with 100 mM NaCl for 3 days, and then Na+ distribution between cytosol and vacuole was quantified by CoroNa Green fluorescent dye imaging. Our major observations were as follows: (1) salinity stress tolerance correlated positively with vacuolar Na+ sequestration ability in the mature root zone but not in the root apex; (2) contrary to expectations, cytosolic Na+ levels in root meristem were significantly higher in salt tolerant than sensitive group, while vacuolar Na+ levels showed an opposite trend. These results are interpreted as meristem cells playing a role of the “salt sensor;” (3) no significant difference in the vacuolar Na+ sequestration ability was found between sensitive and tolerant groups in either transition or elongation zones; (4) the overall Na+ accumulation was highest in the elongation zone, suggesting its role in osmotic adjustment and turgor maintenance required to drive root expansion growth. Overall, the reported results suggest high tissue-specificity of Na+ uptake, signaling, and sequestration in wheat roots. The implications of these findings for plant breeding for salinity stress tolerance are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots

Shabala

Liu

et al. 2015

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Salinity stress is primarily sensed by the roots, and particularly root meristem [49]. As the concentration of salts (particularly Na + , Ca 2+ , Mg 2+ , C1 − , SO 2− and HCO 3− ions) increases, there is a decline in root number, root diameter, and root length [50,51].…”

Section: Effects Of Salt Stress On Potato Growth and Developmentmentioning

confidence: 99%

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Chourasia

Lal

Tiwari

et al. 2021

Life

118

View full text Add to dashboard Cite

Among abiotic stresses, salinity is a major global threat to agriculture, causing severe damage to crop production and productivity. Potato (Solanum tuberosum) is regarded as a future food crop by FAO to ensure food security, which is severely affected by salinity. The growth of the potato plant is inhibited under salt stress due to osmotic stress-induced ion toxicity. Salinity-mediated osmotic stress leads to physiological changes in the plant, including nutrient imbalance, impairment in detoxifying reactive oxygen species (ROS), membrane damage, and reduced photosynthetic activities. Several physiological and biochemical phenomena, such as the maintenance of plant water status, transpiration, respiration, water use efficiency, hormonal balance, leaf area, germination, and antioxidants production are adversely affected. The ROS under salinity stress leads to the increased plasma membrane permeability and extravasations of substances, which causes water imbalance and plasmolysis. However, potato plants cope with salinity mediated oxidative stress conditions by enhancing both enzymatic and non-enzymatic antioxidant activities. The osmoprotectants, such as proline, polyols (sorbitol, mannitol, xylitol, lactitol, and maltitol), and quaternary ammonium compound (glycine betaine) are synthesized to overcome the adverse effect of salinity. The salinity response and tolerance include complex and multifaceted mechanisms that are controlled by multiple proteins and their interactions. This review aims to redraw the attention of researchers to explore the current physiological, biochemical and molecular responses and subsequently develop potential mitigation strategies against salt stress in potatoes.

show abstract

“…Gerrit et al (1996) reported that Na + significantly reduced cell production by decreasing the number of dividing cells in Arabidopsis thaliana [51]. Richardson et al (1996) reported a similar effect of salinity-induced changes in meristematic cells in Solanum tuberosum [52]. The cytotoxicity of chemicals can be determined by measuring the MI, and a decline in the MI to <22%, compared with the negative control, can have a lethal impact on the organism.…”

Section: Discussionmentioning

confidence: 99%

Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)

Wang

Hao

2021

Sustainability

View full text Add to dashboard Cite

The use of drilling waste for land reclamation is a cost-effective way to improve soil fertility and to decrease landfills. However, the potential phytotoxic and cytotoxic effects of this waste on crops have not been investigated in detail. Here, we evaluated the toxicity of spent drilling fluids (SDFs) from a natural gas field using the non-target plant Zea mays L. (maize). Four different concentrations of SDFs (2%, 4%, 6%, and 8%, w/w) were used to test the toxic effects in two soils (aeolian and loessal). Different endpoints, including germination, root elongation, reactive oxygen species (ROS) accumulation, antioxidant activity, mitotic index, and chromosomal abnormalities, were used to test the effects of SDFs after four days of exposure. Higher levels (≥6%) of SDFs inhibited seed germination and root growth, and altered the oxygen status by increasing hydrogen peroxide (H2O2) and inhibiting superoxide ion (O2−) accumulation in the roots. SDFs-induced oxidative stress caused member damage, exacerbated cell injury, and reduced cell viability in the roots, compared with those untreated plants. The plants responded to high SDFs levels (≥6%) by upregulating antioxidants such as peroxidase, superoxide dismutase ascorbate peroxidase, and proline. A reduction in the mitotic index and induction of chromosomal abnormalities in root meristematic cells were indicators of the cytotoxicity of SDFs in maize seedlings. The upregulation of antioxidants due to the change of ROS and the induction of chromosomal abnormalities were more severe in roots grown in aeolian soil than in those grown in loessal soil. The present results provide insight into the mechanism underlying the phytotoxicity and cytotoxicity of SDFs and have implications for land reclamation to minimize deleterious effects on non-target crops.

show abstract

Cell and nuclear degradation in root meristems following exposure of potatoes (Solanum tuberosum L.) to salinity

Cited by 13 publications

References 31 publications

Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots

Linking salinity stress tolerance with tissue-specific Na+ sequestration in wheat roots

Salinity Stress in Potato: Understanding Physiological, Biochemical and Molecular Responses

Effects of Spent Drilling Fluids from Natural Gas Fields on Seed Germination and Root Development of Maize (Zea mays L.)

Contact Info

Product

Resources

About