Cell adhesion on supported lipid bilayers

Andersson, Ann‐Sofie; Glasmästar, Karin; Sutherland, Duncan S.; Lidberg, Ulf; Kasemo, Bengt Herbert

doi:10.1002/jbm.a.10442

Cited by 114 publications

(103 citation statements)

References 45 publications

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“…non-fouling surface, does not promote protein and cell adhesion [28][29][30][31][32] . The reason for this discrepanc y is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…A possible explanation for the differences is considered due to a surface morphology, that is, fluid-like character or rigid character of the surfaces. Many non-fouling surfaces that resist to cell adhesion had a fluid-like character 32 . On the other hand, super-hydrophilic surface of titanium dioxide films 33 or O 2 -treated HMDSO films that showed a high rate of cell attachment had a rigid surface.…”

Section: Discussionmentioning

confidence: 99%

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Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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Surface wettability is an important physicochemical property of biomaterials, and it would be more helpful for understanding this property if a wide range of wettability are employed. This study focused on the effect of surface wettability on fibroblast adhesion over a wide range of wettability using a single material without changing surface topography. Plasma polymerization with hexa methyldisiloxane followed by oxygen (O 2 ) plasma treatment was employed to modify the surfaces. The water contact angle of sample surfaces varied from 106 degrees (hydrophobicity) to almost 0 degrees (super-hydrophilicity). O 2 -functional groups were introduced on polymer surfaces during O 2 -plasma treatment. The cell attachment study confirmed that the more hydrophilic the surface, the more fibroblasts adhered in the initial stage that includes on super-hydrophilic surfaces. Cells spread much more widely on the hydrophilic surfaces than on the hydrophobic surfaces. There was no significant difference in fibroblast proliferation, but cell spreading was much greater on the hydrophilic surfaces. These findings suggest the importance of the surface wettability of biomaterials on initial cell attachment and spreading. The degree of wettability should be taken into account when a new biomaterial is to be employed. Further research of surface wettability on adhesive molecules is necessary for a better understanding of this property.

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“…non-fouling surface, does not promote protein and cell adhesion [28][29][30][31][32] . The reason for this discrepanc y is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

et al. 2006

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“…Supported lipid bilayer (SLB), an artificial mimic of the natural cell membrane, which is compatible with protein incorporation and possesses sustained lateral mobility, [20][21][22][23][24][25][26] has been studied extensively as model system of biological membranes over past decades. Previously, many studies have demonstrated that this biomimetic multifunctional material has great bioinert property and lateral mobility, thus leading to effective resistance of non-specific proteins adsorption and cells adhesion.…”

Section: Introductionmentioning

confidence: 99%

Efficient elusion of viable adhesive cells from a microfluidic system by air foam

Lai

Shao

et al. 2014

Biomicrofluidics

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We developed a new method for releasing viable cells from affinity-based microfluidic devices. The lumen of a microchannel with a U-shape and user-designed microstructures was coated with supported lipid bilayers functionalized by epithelial cell adhesion molecule antibodies to capture circulating epithelial cells of influx solution. After the capturing process, air foam was introduced into channels for releasing target cells and then carrying them to a small area of membrane. The results show that when the air foam is driven at linear velocity of 4.2 mm/s for more than 20 min or at linear velocity of 8.4 mm/s for more than 10 min, the cell releasing efficiency approaches 100%. This flow-induced shear stress is much less than the physiological level (15 dyn/cm 2 ), which is necessary to maintain the intactness of released cells. Combining the design of microstructures of the microfluidic system, the cell recovery on the membrane exceeds 90%. Importantly, we demonstrate that the cells released by air foam are viable and could be cultured in vitro. This novel method for releasing cells could power the microfluidic platform for isolating and identifying circulating tumor cells. V C 2014 AIP Publishing LLC.

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“…In addition, the formation of SLBs is simple 14 and they are highly resistant to nonspecific adsorption by proteins or cells. 12,[15][16][17][18] SLBs consist of a continuous lipid bilayer, 5 nm thick, separated from the substrate by a ϳ1 nm thin water cushion. Previous studies of cell adhesion with SLBs have relied on the use of lipids functionalized with peptides.…”

Section: Introductionmentioning

confidence: 99%

Peptide-presenting two-dimensional protein matrix on supported lipid bilayers: An efficient platform for cell adhesion

et al. 2007

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Understanding and controlling cell adhesion to biomaterials and synthetic materials are important issues in basic research and applied sciences. Supported lipid bilayers ͑SLBs͒ functionalized with cell adhesion peptides linked to lipid molecules are popular platforms of cell adhesion. In this paper, an alternative approach of peptide presentation is presented in which peptides are stereo-selectively linked to proteins self-assembling in a rigid two-dimensional ͑2D͒ matrix on SLBs. Annexin-A5 ͑Anx5͒ was used as prototype protein for its known properties of forming stable and rigid 2D matrices on lipid surfaces. Two types of Anx5-peptide complexes, containing either a RGD or an IKVAV sequence, were synthesized. The authors show that both Anx5-peptide complexes present the same properties of binding and 2D organization on lipid surfaces as Anx5, when investigated by quartz crystal microbalance with dissipation monitoring, atomic force microscopy, and transmission electron microscopy techniques. Anx5-RGD and Anx5-IKVAV 2D matrices were found to promote specific adhesion of human saphenous vein endothelial cells and mouse embryonic stem cells, respectively. The influence of the surface density of exposed peptides on cell adhesion was investigated, showing that cells attach to Anx5-peptide matrices when the average distance between peptides is smaller than about 60 nm. This cell adhesion platform provides control of the orientation and density of cell ligands, opening interesting possibilities for future applications.

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Cell adhesion on supported lipid bilayers

Cited by 114 publications

References 45 publications

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Adhesion of mouse fibroblasts on hexamethyldisiloxane surfaces with wide range of wettability

Efficient elusion of viable adhesive cells from a microfluidic system by air foam

Peptide-presenting two-dimensional protein matrix on supported lipid bilayers: An efficient platform for cell adhesion

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