Cell adhesion molecules

Murray, Paul G.; Frampton, Geoffrey; Nelson, PN

doi:10.1136/bmj.319.7206.332

Cited by 24 publications

(11 citation statements)

References 14 publications

(10 reference statements)

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“…CD43, a transmembrane sialoglycoprotein expressed exclusively on hematopoietic cells [44] and a potential CD54 ligand, interacts with cytoskeletal proteins and is involved in signaling regulating cell activation, proliferation and survival [45]. CD44, CD50 and CD54 are glycoproteins with roles in cell-to-cell adhesion [46]. Blocking with either anti-CD44, anti-CD50 or anti-CD133 antibodies did not affect MkMP binding and/or uptake (data not shown).…”

Section: Resultsmentioning

confidence: 99%

How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

Jiang

Kao

Papoutsakis

2017

Journal of Controlled Release

122

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Megakaryocytic microparticles (MkMPs), the most abundant MPs in circulation, can induce the differentiation of hematopoietic stem and progenitor cells (HSPCs) into functional megakaryocytes. This MkMP capability could be explored for applications in transfusion medicine but also for delivery of nucleic acids and other molecules to HSPCs for targeted molecular therapy. Understanding how MkMPs target, deliver cargo and alter the fate of HSPCs is important for exploring such applications. We show that MkMPs, which are distinct from Mk exosomes (MkExos), target HSPCs with high specificity since they have no effect on other ontologically or physiologically related cells, namely mesenchymal stem cells, endothelial cells or granulocytes. The outcome is also specific: only cells of the megakaryocytic lineage are generated. Observation of intact fluorescently-tagged MkMPs inside HSPCs demonstrates endocytosis as one mechanism of cargo delivery. Fluorescent labeling and scanning electron microscopy (SEM) imaging show that direct fusion of MkMPs into HSPCs is also engaged in cargo delivery. SEM imaging detailed the membrane-fusion process in four stages leading to full adsorption of MkMPs into HSPCs. Specifically, macropinocytosis and lipid raft-mediated were shown here as mechanisms of MkMP uptake by HSPC. In contrast, the ontologically related platelet-derived MPs (PMPs) cannot be taken up by HSPCs although they bind to and induce HSPC aggregation. We show that platelet-like thrombin activation is apparently responsible for the different biological effects of MkMPs versus PMPs on HSPCs. We show that HSPC uropods are the preferential site for MkMP binding, and that CD54 (ICAM-1), CD11b, CD18 and CD43, localized on HSPC uropods, are involved in MkMP binding to HSPCs. Finally, we show that MkMP RNA is largely responsible for HSPC programming into Mk differentiation.

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Section: Resultsmentioning

confidence: 99%

How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

Jiang

Kao

Papoutsakis

2017

Journal of Controlled Release

122

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“…In a multicellular organism, cells communicate with each other and the ECM through cell adhesion molecules (CAMs). CAMs comprise different groups such as the CD44 family, selectins, the immunoglobulin superfamily, cadherins, and integrins [108]. Integrins represent the major link between cells and the ECM.…”

Section: Matrix Stiffness and Integrin Signalingmentioning

confidence: 99%

Interplay Between LOX Enzymes and Integrins in the Tumor Microenvironment

Amendola

Reuten

Erler

2019

Cancers

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Members of the lysyl oxidase (LOX) family are secreted copper-dependent amine oxidases that catalyze the covalent crosslinking of collagens and elastin in the extracellular matrix (ECM), an essential process for the structural integrity of all tissues. LOX enzymes can also remodel the tumor microenvironment and have been implicated in all stages of tumor initiation and progression of many cancer types. Changes in the ECM can influence several cancer cell phenotypes. Integrin adhesion complexes (IACs) physically connect cells with their microenvironment. This review article summarizes the main findings on the role of LOX proteins in modulating the tumor microenvironment, with a particular focus on how ECM changes are integrated by IACs to modulate cells behavior. Finally, we discuss how the development of selective LOX inhibitors may lead to novel and effective therapies in cancer treatment.

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“…Moreover, MMPs disassemble an impaired ECM, regulate extracellular tissue signalling networks, and induce angiogenesis and immune responses [ 29 ]. Alternatively, adhesion molecules facilitate the reassembly of the ECM via cell-to-cell and cell-to-ECM cross-talk [ 30 ]. Cell adhesion molecule include the integrins ( ITGs ), selectins, intercellular adhesion molecules ( ICAM ), neural cell adhesion molecules ( NCAM ), vascular cell adhesion molecules ( VCAM ), and the catenins [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively, adhesion molecules facilitate the reassembly of the ECM via cell-to-cell and cell-to-ECM cross-talk [ 30 ]. Cell adhesion molecule include the integrins ( ITGs ), selectins, intercellular adhesion molecules ( ICAM ), neural cell adhesion molecules ( NCAM ), vascular cell adhesion molecules ( VCAM ), and the catenins [ 30 , 31 ]. These molecules, in conjunction with growth factors and leucocytes, generate an ideally organised ECM infrastructure, which synchronises cell growth and optimises wound healing [ 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Leucocyte-Rich Platelet-Rich Plasma Enhances Fibroblast and Extracellular Matrix Activity: Implications in Wound Healing

Devereaux

Dargahi

Fraser

et al. 2020

IJMS

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Background: Platelet-rich plasma (PRP) is an autologous blood product that contains a high concentration of platelets and leucocytes, which are fundamental fibroblast proliferation agents. L iterature has emerged that offers contradictory findings about leucocytes within PRP. Herein, we elucidated the effects of highly concentrated leucocytes and platelets on human fibroblasts. Methods: Leucocyte-rich, PRP (LR-PRP) and leucocyte-poor, platelet-poor plasma (LP-PPP) were compared to identify their effects on human fibroblasts, including cell proliferation, wound healing and extracellular matrix and adhesion molecule gene expressions. Results: The LR-PRP exhibited 1422.00 ± 317.21 × 103 platelets/µL and 16.36 ± 2.08 × 103 white blood cells/µL whilst the LP-PPP demonstrated lower concentrations of 55.33 ± 10.13 × 103 platelets/µL and 0.8 ± 0.02 × 103 white blood cells/µL. LR-PRP enhanced fibroblast cell proliferation and cell migration, and demonstrated either upregulation or down-regulation gene expression profile of the extracellular matrix and adhesion molecules. Conclusion: LR-PRP has a continuous stimulatory anabolic and ergogenic effect on human fibroblast cells.

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Cell adhesion molecules

Cited by 24 publications

References 14 publications

How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells?

Interplay Between LOX Enzymes and Integrins in the Tumor Microenvironment

Leucocyte-Rich Platelet-Rich Plasma Enhances Fibroblast and Extracellular Matrix Activity: Implications in Wound Healing

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