Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.

Komohara, Yoshihiro; Ma, Chaoya; Yano, Hiromichi; Pan, Cheng; Horlad, Hasita; Saito, Yoichi; Ohnishi, Koji; Fujiwara, Yukio; Okuno, Yutaka; Nosaka, Kisato; Shimosaki, Shunsuke; Morishita, Kazuhiro; Matsuoka, Masao; Wakayama, Tomohiko; Takeya, Motohiro

doi:10.3960/jslrt.17003

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Interestingly, DLX6-AS1 was reported to enhance osteosarcoma stemness through regulation of miR-129-5p/DLK1, suggested that DLX6-AS1 might correlate with cancer stemness [13]. In addition, cell adhesion molecule 1 (CADM1), whose expression has been extensively found in lung, brain, testis, liver, and some cancer cells [14], has been observed to be downregulated in HCC cells, serving as a propellant of tumorigenesis of HCC [15]. Research has provided evidence that HCC is more likely to recur in patients with extensive CADM1 methylation compared to patients with less or without CADM1 methylation [16].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Down-regulated lncRNA DLX6-AS1 inhibits tumorigenesis through STAT3 signaling pathway by suppressing CADM1 promoter methylation in liver cancer stem cells

Zheng

Zhang

et al. 2019

J Exp Clin Cancer Res

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Background Liver cancer stem cells (LCSCs) are a small subset of cells characterized by unlimited self-renewal, cell differentiation, and uncontrollable cellular growth. LCSCs are also resistant to conventional therapies and are thus believed to be held responsible for causing treatment failure of hepatocellular carcinoma (HCC). It has been recently found that long non-coding RNAs (lncRNAs) are important regulators in HCC. This present study aims to explore the underlying mechanism of how lncRNA DLX6-AS1 influences the development of LCSCs and HCC. Methods A microarray-based analysis was performed to initially screen differentially expressed lncRNAs associated with HCC. We then analyzed the lncRNA DLX6-AS1 levels as well as CADM1 promoter methylation. The mRNA and protein expression of CADM1, STAT3, CD133, CD13, OCT-4, SOX2, and Nanog were then detected. We quantified our results by evaluating the spheroid formation, proliferation, and tumor formation abilities, as well as the proportion of tumor stem cells, and the recruitment of DNA methyltransferase (DNMT) in LCSCs when lncRNA DLX6-AS1 was either overexpressed or silenced. Results LncRNA DLX6-AS1 was upregulated in HCC. The silencing of lncRNA DLX6-AS1 was shown to reduce and inhibit spheroid formation, colony formation, proliferation, and tumor formation abilities, as well as attenuate CD133, CD13, OCT-4, SOX2, and Nanog expression in LCSCs. Furthermore, downregulation of lncRNA DLX6-AS1 contributed to a reduction in CADM1 promoter methylation via suppression of DNMT1, DNMT3a, and DNMT3b in LCSCs and inactivating the STAT3 signaling pathway. Conclusion This study demonstrated that down-regulated lncRNA DLX6-AS1 may inhibit the stem cell properties of LCSCs through upregulation of CADM1 by suppressing the methylation of the CADM1 promoter and inactivation of the STAT3 signaling pathway. Electronic supplementary material The online version of this article (10.1186/s13046-019-1239-3) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Down-regulated lncRNA DLX6-AS1 inhibits tumorigenesis through STAT3 signaling pathway by suppressing CADM1 promoter methylation in liver cancer stem cells

Zheng

Zhang

et al. 2019

J Exp Clin Cancer Res

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“…Transcriptional profiling using Affymetrix RNA microarrays of IL-10–producing versus nonproducing T F cells revealed highly comparable transcriptomes (Fig. 2 a), with only a few differentially expressed transcripts including IL-10 , C-C motif chemokine 4-like ( CCL4L2 ), T-lymphocyte maturation-associated protein ( MAL ), microRNA424 ( MIR424 ; reported to be an activator of TGF-β signaling; Li et al, 2014), lymphoid enhancer binding factor 1 ( LEF1 ; a transcription factor important for T FH differentiation; Choi et al, 2015), and the surface receptor cell adhesion molecule 1 ( CADM1 ), which has been previously reported to mark a population of adult T cell leukemia/lymphoma (Komohara et al, 2017). To evaluate whether CADM1 expression could identify the IL-10–producing CD25 + T F cells, we stained human tonsils with an anti-CADM1 antibody together with T F - and T reg cell–specific markers.…”

Section: Resultsmentioning

confidence: 99%

Regulatory roles of IL-10–producing human follicular T cells

Cañete

Sweet

González-Figueroa

et al. 2019

Journal of Experimental Medicine

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Mucosal lymphoid tissues such as human tonsil are colonized by bacteria and exposed to ingested and inhaled antigens, requiring tight regulation of immune responses. Antibody responses are regulated by follicular helper T (TFH) cells and FOXP3+ follicular regulatory T (TFR) cells. Here we describe a subset of human tonsillar follicular T cells identified by expression of TFH markers and CD25 that are the main source of follicular T (TF) cell–derived IL-10. Despite lack of FOXP3 expression, CD25+ TF cells resemble T reg cells in high CTLA4 expression, low IL-2 production, and their ability to repress T cell proliferation. CD25+ TF cell–derived IL-10 dampens induction of B cell class-switching to IgE. In children, circulating total IgE titers were inversely correlated with the frequencies of tonsil CD25+ TF cells and IL-10–producing TF cells but not with total T reg cells, TFR, or IL-10–producing T cells. Thus, CD25+ TF cells emerge as a subset with unique T and B cell regulatory activities that may help prevent atopy.

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“…The monocytes were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and GM-CSF (10 ng/ml, WAKO, Tokyo, Japan) for 7 days in order to differentiate them into macrophages. 18 Resident peritoneal macrophages from mice (8-10 weeks of age) were obtained by peritoneal lavage using 6 ml of PBS. Cells were incubated in DMEM medium with 10% FBS.…”

Section: Cells and Cell Culture Conditionsmentioning

confidence: 99%

Natural compounds that regulate lymph node sinus macrophages: Inducing an anti-tumor effect by regulating macrophage activation

Fujiwara

Saito

Shiota

et al. 2018

JCEH

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Cell adhesion molecule-1 (CADM1) expressed on adult T-cell leukemia/lymphoma cells is not involved in the interaction with macrophages.

Cited by 6 publications

References 26 publications

RETRACTED ARTICLE: Down-regulated lncRNA DLX6-AS1 inhibits tumorigenesis through STAT3 signaling pathway by suppressing CADM1 promoter methylation in liver cancer stem cells

RETRACTED ARTICLE: Down-regulated lncRNA DLX6-AS1 inhibits tumorigenesis through STAT3 signaling pathway by suppressing CADM1 promoter methylation in liver cancer stem cells

Regulatory roles of IL-10–producing human follicular T cells

Natural compounds that regulate lymph node sinus macrophages: Inducing an anti-tumor effect by regulating macrophage activation

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