Cell adhesion mechanisms on laterally mobile polymer films

Kourouklis, Andreas P.; Lerum, Ronald V.; Bermudez, Harry

doi:10.1016/j.biomaterials.2014.02.052

Cited by 30 publications

(31 citation statements)

References 58 publications

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“…What are the effects of movable ligands versus rigidly anchored ligands? Many common substrates are limited in their ability to recapitulate the intrinsic flexibility of the ECM; therefore, these effects have been less studied than the stiffness response [149]. Recently, several novel substrates and mathematical models have been developed to probe these effects.…”

Section: Mechanobiology Of Adhesion Revealed Using Supported Lipidmentioning

confidence: 99%

Supported lipid bilayer platforms to probe cell mechanobiology

Glazier¹,

Salaita²

2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Mammalian and bacterial cells sense and exert mechanical forces through the process of mechanotransduction, which interconverts biochemical and physical signals. This is especially important in contact-dependent signaling, where ligand-receptor binding occurs at cell-cell or cell-ECM junctions. By virtue of occurring within these specialized junctions, receptors engaged in contact-dependent signaling undergo oligomerization and coupling with the cytoskeleton as part of their signaling mechanisms. While our ability to measure and map biochemical signaling within cell junctions has advanced over the past decades, physical cues remain difficult to map in space and time. Recently, supported lipid bilayer (SLB) technologies have emerged as a flexible platform to measure and manipulate membrane receptor mechanotransduction, allowing one to mimic cell-cell junctions. Changing the lipid composition and underlying substrate tunes bilayer fluidity, and lipid and ligand micro- and nano-patterning spatially control positioning and clustering of receptors. Patterning metal gridlines within SLBs introduces corrals that confine lipid mobility and introduce mechanical resistance. Here we review fundamental SLB mechanics and how SLBs can be engineered as tunable cell substrates for mechanotransduction studies. Finally, we highlight the impact of this work in understanding the biophysical mechanisms of cell adhesion.

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Section: Mechanobiology Of Adhesion Revealed Using Supported Lipidmentioning

confidence: 99%

Supported lipid bilayer platforms to probe cell mechanobiology

Glazier¹,

Salaita²

2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…The dot widths (i.e., diameters) remained constant at~20 μm in all cases for the entire 18-h time frame, irrespective of whether the films were neat or doped ( Figure c). Moreover, it is shown from previous studies that the lateral diffusion coefficients of the neat and doped films are 1×10 -10 and 3.5×10 -10 cm 2 /s, respectively [27]. Such lateral diffusion of FN-labeled polymer from a single dot of uniform concentration would result in the dot width increasing by more than 50% of its initial size within 3 h [31], which is not observed.…”

Section: Resultsmentioning

confidence: 85%

“…To increase the lateral mobility of the polymer film, we introduced a small amount (1 mol %) of polyisobutylene homopolymer during the LS fabrication step [27]; such films are hereafter referred to as "doped. " Films without any added polyisobutylene have lower mobility and are referred to as "neat. "…”

Section: Resultsmentioning

confidence: 99%

Fibronectin Patterning of Viscous Polymer films by Microcontact Printing

et al. 2016

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“…Immobilized proteins (e.g., laminin), peptide sequences (e.g., RGD) and carbohydrates (e.g., galactose) have been used to control cell behavior [47,59]. The fibronectin-derived peptide sequence RGD has received particular attention to promote integrin-mediated cell adhesion to artificial surfaces [60].…”

Section: Chemicalmentioning

confidence: 99%

Responsive Cell–Material Interfaces

Dhowre

Rajput

Russell

et al. 2015

Nanomedicine (Lond.)

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Major design aspects for novel biomaterials are driven by the desire to mimic more varied and complex properties of a natural cellular environment with man-made materials. The development of stimulus responsive materials makes considerable contributions to the effort to incorporate dynamic and reversible elements into a biomaterial. This is particularly challenging for cell-material interactions that occur at an interface (biointerfaces); however, the design of responsive biointerfaces also presents opportunities in a variety of applications in biomedical research and regenerative medicine. This review will identify the requirements imposed on a responsive biointerface and use recent examples to demonstrate how some of these requirements have been met. Finally, the next steps in the development of more complex biomaterial interfaces, including multiple stimuli-responsive surfaces, surfaces of 3D objects and interactive biointerfaces will be discussed.

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Cell adhesion mechanisms on laterally mobile polymer films

Cited by 30 publications

References 58 publications

Supported lipid bilayer platforms to probe cell mechanobiology

Supported lipid bilayer platforms to probe cell mechanobiology

Fibronectin Patterning of Viscous Polymer films by Microcontact Printing

Responsive Cell–Material Interfaces

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