Cell Adhesion Behavior in 3D Hydrogel Scaffolds Functionalized with <scp>D</scp>‐ or <scp>L</scp>‐Aminoacids

Benson, Kathrin; Galla, Hans‐Joachim; Kehr, Nermin Seda

doi:10.1002/mabi.201300485

Cited by 32 publications

(17 citation statements)

References 37 publications

(37 reference statements)

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“…EV secretion dynamics from 3D-cultured cells significantly differ from 2D culture system. In order to culture in-vivo like 3D cells, we choose to use peptide hydrogel as the scaffolding material which has high biocompatibility and self-healing property for transforming between liquid and gelation [48][49][50] . This peptide hydrogel is stable in neutral pH and remains as gel formation in a wide range of temperatures from 2 °C to 80 °C 51,52 .…”

Section: Resultsmentioning

confidence: 99%

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

Thippabhotla

Zhong

2019

Sci Rep

168

137

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For studying cellular communications ex-vivo, a two-dimensional (2D) cell culture model is currently used as the “gold standard”. 2D culture models are also widely used in the study of RNA expression profiles from tumor cells secreted extracellular vesicles (EVs) for tumor biomarker discovery. Although the 2D culture system is simple and easily accessible, the culture environment is unable to represent in vivo extracellular matrix (ECM) microenvironment. Our study observed that 2D- culture derived EVs showed significantly different profiles in terms of secretion dynamics and essential signaling molecular contents (RNAs and DNAs), when compared to the three-dimensional (3D) culture derived EVs. By performing small RNA next-generation sequencing (NGS) analysis of cervical cancer cells and their EVs compared with cervical cancer patient plasma EV-derived small RNAs, we observed that 3D- culture derived EV small RNAs differ from their parent cell small RNA profile which may indicate a specific sorting process. Most importantly, the 3D- culture derived EV small RNA profile exhibited a much higher similarity (~96%) to in vivo circulating EVs derived from cervical cancer patient plasma. However, 2D- culture derived EV small RNA profile correlated better with only their parent cells cultured in 2D. On the other hand, DNA sequencing analysis suggests that culture and growth conditions do not affect the genomic information carried by EV secretion. This work also suggests that tackling EV molecular alterations secreted into interstitial fluids can provide an alternative, non-invasive approach for investigating 3D tissue behaviors at the molecular precision. This work could serve as a foundation for building precise models employed in mimicking in vivo tissue system with EVs as the molecular indicators or transporters. Such models could be used for investigating tumor biomarkers, drug screening, and understanding tumor progression and metastasis.

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Section: Resultsmentioning

confidence: 99%

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

Thippabhotla

Zhong

2019

Sci Rep

168

137

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“…These mechanical properties include strain, shear stress, compressive modulus and mass swelling ratio. It is well understood that cell adhesion is significantly affected by the dynamic interactions between cells and hydrogels (Dou et al 2012; Benson et al 2014). In fact, mechanical properties are considered to be highly essential for soft tissues, such as cartilage and skin, because the functions of such tissues mainly rely on their mechanical properties (Hutmacher 2000; Kim et al 2012).…”

Section: Materials For Bioprintingmentioning

confidence: 99%

3D bioprinting for engineering complex tissues

Mandrycky

Wang

Kim

2016

Biotechnology Advances

1,310

1,046

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Bioprinting is a 3D fabrication technology used to precisely dispense cell-laden biomaterials for the construction of complex 3D functional living tissues or artificial organs. While still in its early stages, bioprinting strategies have demonstrated their potential use in regenerative medicine to generate a variety of transplantable tissues, including skin, cartilage, and bone. However, current bioprinting approaches still have technical challenges in terms of high-resolution cell deposition, controlled cell distributions, vascularization, and innervation within complex 3D tissues. While no one-size-fits-all approach to bioprinting has emerged, it remains an on-demand, versatile fabrication technique that may address the growing organ shortage as well as provide a high-throughput method for cell patterning at the micrometer scale for broad biomedical engineering applications. In this review, we introduce the basic principles, materials, integration strategies and applications of bioprinting. We also discuss the recent developments, current challenges and future prospects of 3D bioprinting for engineering complex tissues. Combined with recent advances in human pluripotent stem cell technologies, 3D-bioprinted tissue models could serve as an enabling platform for high-throughput predictive drug screening and more effective regenerative therapies.

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“…In order to culture in-vivo like 3D cells, we choose to use peptide hydrogel as the scaffolding material which has high biocompatibility and self-healing property for transforming between liquid and gelation [48][49][50] . This peptide hydrogel is stable in neutral pH and remains as gel formation in a wide range of temperatures from 2° C to 80° C 51,52 .…”

Section: Ev Secretion Dynamics From 3d Cultured Cells Significantly Dmentioning

confidence: 99%

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

Thippabhotla

Wei

Zhong

et al. 2019

Preprint

View full text Add to dashboard Cite

For studying cellular communications ex-vivo, a two-dimensional (2D) cell culture model is currently used as the "gold standard". 2D culture models are also widely used in the study of RNA expression profiles from tumor cells secreted extracellular vesicles (EVs) for tumor biomarker discovery. Although the 2D culture system is simple and easily accessible, the culture environment is unable to represent in vivo extracellular matrix (ECM) microenvironment. Our study observed that 2D culture-derived EVs showed significantly different profiles in terms of secretion dynamics and essential signaling molecular contents (RNAs and DNAs), when compared to the three-dimensional (3D) culture-derived EVs. By performing small RNA next-generation sequencing (NGS) analysis of cervical cancer cells and their EVs compared with cervical cancer patient plasma EV-derived small RNAs, we observed that 3D culture-derived EV small RNAs differ from their parent cell small RNA profile which may indicate a specific sorting process. Most importantly, the 3D culture derived EV small RNA profile exhibited a much higher similarity (~96%) to in vivo circulating EVs derived from cervical cancer patient plasma. However, 2D culture derived EV small RNA profile correlated better with only their parent cells cultured in 2D. On the other hand, DNA sequencing analysis suggests that culture and growth conditions do not affect the genomic information carried by EV secretion. This work also suggests that tackling EV molecular alterations secreted into interstitial fluids can provide an alternative, non-invasive approach for investigating 3D tissue behaviors at the molecular precision. This work could serve as a foundation for building precise models employed in mimicking in vivo tissue system with EVs as the molecular indicators or transporters. Such models could be used for investigating tumor biomarkers, drug screening, and understanding tumor progression and metastasis.

show abstract

Cell Adhesion Behavior in 3D Hydrogel Scaffolds Functionalized with D‐ or L‐Aminoacids

Cited by 32 publications

References 37 publications

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

3D bioprinting for engineering complex tissues

3D cell culture stimulates the secretion of in vivo like extracellular vesicles

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