Cell Adhesion and Proliferation Patterns on Mixed Self-assembled Monolayers Carrying Various Patios of Hydroxyl and Methyl Groups

Fuse, Yoshihiko; Hirata, Isao; Kurihara, Hidemi; Okazaki, Masanori

doi:10.4012/dmj.26.814

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…Therefore in this study, SAMs were modified with four functional groups, -COOH, -NH2, -CH3, and -OH [12][13][14] , on gold wafer surfaces. Our next focus was to conduct a basic research on chemical analysis, where surface plasmon resonance (SPR) analysis -in addition to SEM, XRD, ESCA-was used for real-time observation of the early initial deposition of calcium phosphate.…”

Section: Introductionmentioning

confidence: 99%

Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH2, CH3, and OH functions

et al. 2010

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Hydroxyapatite formation was examined at the surface of self-assembled monolayers (SAMs) modified with four functional groups, -COOH, -NH2, -CH3, and -OH. For COOH-SAM and NH2-SAM, scanning electron spectroscopic observation showed that flake-like sheet crystals covered the whole wafer and small broccoli-like crystals were observed occasionally on the flake-like crystal base layer. For CH3-SAM and OH-SAM, no flake-like sheet crystals were observed; broccoli-like crystals were observed in a dispersed manner for CH3-SAM, but in localized spots for OH-SAM. X-ray diffraction patterns showed a strong apatite pattern oriented toward the c-axis direction for COOH-SAM. ESCA analysis revealed distinct Ca, P, O peaks for COOH-, NH2-, CH3-, and OH-SAM. Surface plasmon resonance (SPR) analysis indicated that during the supply of supersaturated calcium phosphate solution, the deposition of precipitates increased monotonically with time for COOH-SAM, increased slightly for NH2-SAM, but little increase in deposition was detected for CH3-SAM and OH-SAM.

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Section: Introductionmentioning

confidence: 99%

Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH2, CH3, and OH functions

et al. 2010

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“…In contrast, the adhesion area and aspect ratio of C2C12 cells following proliferation for 48 h on fMIL-53 (Al) were 1573 μm 2 and 3.4, respectively, and after 72 h, they were 1525 μm 2 and 2.3, respectively, indicating that the morphological characteristics of the cells were unaltered by this scaffold. Thus, cells cultured on fMIL-53 (Al) maintained good proliferation and morphological characteristics, demonstrating that the cell properties of fMIL-53 (Al) were comparable with those of conventional cell culture scaffolds . Large differences in the extracellular microenvironment between fMIL-53 (Al) and the polyimide film could be due to the serum protein adsorption, retention, and replenishment capabilities of fMIL-53 (Al).…”

Section: Resultsmentioning

confidence: 83%

Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities

Katayama

Tanaka

Tsuruoka

et al. 2022

ACS Appl. Mater. Interfaces

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Metal–organic frameworks (MOFs) are porous materials with adsorption, storage, and separation capabilities due to their high specific surface areas and large pore volumes. MOFs are thus used in biomedical applications, and MOF nanoparticles have been widely studied as nanocarriers for drug delivery systems. Several research groups recently reported that specific MOF nanoparticles can adsorb and retain proteins, suggesting to us that MOF nanoparticles may have advantages as novel cell culture scaffolds. However, MOF nanoparticles cannot be used as two-dimensional scaffolds for cells. We therefore established a bottom-up technique to construct two-dimensional MOFs [MIL-53 (Al)] on polymer films. The developed two-dimensional MIL-53 (Al) film [fMIL-53 (Al)] exhibited high serum protein adsorption, retention, and replenishment capabilities as compared to conventional cell culture scaffolds. β-Galactosidase, used as a model protein, adsorbed on fMIL-53 (Al) exhibited original enzymatic activity, indicating that proteins are not denatured during the adsorption process. The viability of mouse myoblast cells (C2C12) cultured on fMIL-53 (Al) was 100%, indicating the cell compatibility of fMIL-53 (Al). Importantly, C2C12 cells cultured on serum protein-preadsorbed fMIL-53 (Al) exhibited excellent long-term adhesion, morphology, and proliferation even in a medium lacking serum proteins, demonstrating an important advantage of fMIL-53 (Al) as a cell culture scaffold, given that conventional cell culture scaffolds typically require a serum-containing medium to support stable cell adhesion and proliferation. To our knowledge, this is the first report regarding the application of MOFs as cell culture scaffolds and will serve as a starting point for studying two- and three-dimensional MOF-based cellular scaffolds for cell culture systems and for in vitro and in vivo tissue engineering.

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“…Several surface properties have been proposed to influence protein adsorption and cell behavior: charge, 13 topography, 13 surface energy, 14 and surface chemistry. 15 In this work, various techniques (antibody recognition, fluorescent labeling, and force spectroscopy) were used to investigate the capability of Fn to adsorb on four different materials and to induce the adhesion of preosteoblasts. Fn is one of the most studied proteins of the ECM: it plays a crucial role in the adhesion, spreading, proliferation, and differentiation of many cell types to ECM and artificial surfaces, including polystyrene supports for tissue culture.…”

Section: Introductionmentioning

confidence: 99%

“…The rapid adsorption of proteins effectively provides the cell a biological interpretation of the structure and composition of the foreign surface. Several surface properties have been proposed to influence protein adsorption and cell behavior: charge, topography, surface energy, and surface chemistry …”

Section: Introductionmentioning

confidence: 99%

Probing Fibronectin−Surface Interactions: A Multitechnique Approach

et al. 2008

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The development of adhesive as well as antiadhesive surfaces is essential in various biomaterial applications. In this study, we have used a multidisciplinary approach that combines biological and physicochemical methods to progress in our understanding of cell-surface interactions. Four model surfaces have been used to investigate fibronectin (Fn) adsorption and the subsequent morphology and adhesion of preosteoblasts. Such experimental conditions lead us to distinguish between anti- and proadhesive substrata. Our results indicate that Fn is not able to induce cell adhesion on antiadhesive materials. On adhesive substrata, Fn did not increase the number of adherent cells but favored their spreading. This work also examined Fn-surface interactions using ELISA immunoassays, fluorescent labeling of Fn, and force spectroscopy with Fn-modified tips. The results provided clear evidence of the advantages and limitations of each technique. All of the techniques confirmed the important adsorption of Fn on proadhesive surfaces for cells. By contrast, antiadhesive substrata for cells avoided Fn adsorption. Furthermore, ELISA experiments enabled us to verify the accessibility of cell binding sites to adsorbed Fn molecules.

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Cell Adhesion and Proliferation Patterns on Mixed Self-assembled Monolayers Carrying Various Patios of Hydroxyl and Methyl Groups

Cited by 13 publications

References 36 publications

Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH2, CH3, and OH functions

Chemical analyses of hydroxyapatite formation on SAM surfaces modified with COOH, NH2, CH3, and OH functions

Two-Dimensional Metal–Organic Framework-Based Cellular Scaffolds with High Protein Adsorption, Retention, and Replenishment Capabilities

Probing Fibronectin−Surface Interactions: A Multitechnique Approach

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