Cell adhesion and growth on synthetic elastomeric matrices containing ARG‐GLY–ASP–SER–³

Abstract

Synthetic elastomeric polypeptide matrices based on the repeating amino acid sequences of elastin have biophysical and biological properties which are favorable for prosthetic materials. An important requirement envisaged for some applications is the ability to support cell adhesion and growth. The X20-poly-(GVGVP), the gamma-irradiation cross-linked elastomeric matrix based on the repeating pentamer Val-Pro-Gly-Val-Gly, and X20-poly[n(GVGVP), (GRGDSP)] containing the covalently incorporated cell adhesion sequence Arg-Gly-Asp-Ser (RGDS) were synthesized. These matrices were tested for their ability to support the adhesion and growth of bovine aortic endothelial cells and of bovine ligamentum nuchae fibroblasts. Adhesion experiments carried out in albumin-containing media showed that matrices containing 60:1, 40:1, and 20:1 ratios of (GVGVP):(GRGDSP) supported maximal cell attachment, that matrices containing 100:1 exhibited an intermediate level of attachment and that matrices composed of 500:1 and (GVGVP) alone were very poor supports for cell attachment. Serum in the media promoted submaximal cell attachment to X20-poly(GVGVP) but did not permit substantial cell growth. Cell growth was supported by matrices having high ratios of (GRGDSP). Ratios of 60:1, 40:1, and 20:1 supported three population doublings of endothelial cells over 3 days resulting in confluent matrix-adherent monolayers. Ratios of 40:1 and 20:1 similarly supported the growth of fibroblasts.

Peptide-based biopolymers in biomedicine and biotechnology

“…Biological materials such as elastin-based polypeptides, collagens derived from extracellular matrices, fibrins, and spider silk proteins [59,62,153,[183][184][185][186][187] are useful for tissue engineering because they have good chemical compatibility in aqueous solutions, good in vivo biocompatibility, a controllable degradation rate in vivo, the ability to break down into natural amino acids that can be metabolized by the body, and minimal cytotoxicity, immune response, and inflammation [174,[188][189][190][191][192][193][194]. In addition, biopolymers can be easily functionalized to enhance their interactions with cells and provide an optimal platform for cellular activities and tissue functions.…”

Molekulare Maschinen: Wie Bewegung und andere Funktionen lebender Organismen aus reversiblen chemischen Änderungen entstehen

“…Zellen iiber einen Zeitraum von drei Tagen vermehren konnten. Die Zellen adhirierten, breiteten sich aus und vermehrten sich (aus [71] Carbamideinheiten aus Asparagin-und Glntaminresten enthalten (C). Am ijbergang zur wiifirigen Phase werden die CONH,-hydrolytisch zu COO--Gruppen gespalten; dadurch erhLlt die Oberfliichenschicht ein hoheres T, und quillt bis zum zehnfachen Volumen (E).…”

Dynamic, 3D‐Pattern Formation Within Enzyme‐Responsive Hydrogels

“…[16,17] The structural domains are based upon a well-known sequence borrowed from the protein elastin that imparts properties of mechanical resilience and elasticity. [18,19] The intermittent active domains contain short amino acid sequences, previously selected through analysis of a four-amino acid peptide library, [20] that exhibit varying susceptibility to cleavage by the enzymes, tissue plasminogen activator (tPA), and urokinase plasminogen activator (uPA). These enzymes are members of the serine-protease family, are primarily produced in vivo by endothelial and neuronal cells, and have been linked to physiological roles in thrombolysis (blood-clot dissolution) and extracellular-matrix (ECM) degradation.…”