Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria

Ivanova, Elena P.; Webb, Hayden K.; Christen, Richard; Zhukova, Natalia V.; Kurilenko, Valeriya V.; Kalinovskaya, Nataliya I.; Crawford, Russell J.

doi:10.1099/ijs.0.014159-0

Cited by 29 publications

(25 citation statements)

References 34 publications

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“…Unfortunately, it was not possible to localize M cells in the co-culture, since the large hydrated cells were highly flexible and only rough cell contours could be detected. Thus, cells were cultivated on transwell ® inserts and scanned in contact mode in a semi-dry state at ambient temperatures [ 37 – 38 ]. The results revealed that Caco-2 cells show the typical microvilli-rich intestinal brush border upon reaching confluence.…”

Section: Resultsmentioning

confidence: 99%

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

Schimpel

Werzer

Frőhlich

et al. 2015

Beilstein J. Nanotechnol.

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SummaryThe small intestine is a complex system that carries out various functions. The main function of enterocytes is absorption of nutrients, whereas membranous cells (M cells) are responsible for delivering antigens/foreign substances to the mucosal lymphoid tissues. However, to get a fundamental understanding of how cellular structures contribute to physiological processes, precise knowledge about surface morphologies, cytoskeleton organizations and biomechanical properties is necessary. Atomic force microscopy (AFM) was used here as a powerful tool to study surface topographies of Caco-2 cells and M cells. Furthermore, cell elasticity (i.e., the mechanical response of a cell on a tip indentation), was elucidated by force curve measurements. Besides elasticity, adhesion was evaluated by recording the attraction and repulsion forces between the tip and the cell surface. Organization of F-actin networks were investigated via phalloidin labeling and visualization was performed with confocal laser scanning fluorescence microscopy (CLSM) and scanning electron microscopy (SEM). The results of these various experimental techniques revealed significant differences in the cytoskeleton/microvilli arrangements and F-actin organization. Caco-2 cells displayed densely packed F-actin bundles covering the entire cell surface, indicating the formation of a well-differentiated brush border. In contrast, in M cells actins were arranged as short and/or truncated thin villi, only available at the cell edge. The elasticity of M cells was 1.7-fold higher compared to Caco-2 cells and increased significantly from the cell periphery to the nuclear region. Since elasticity can be directly linked to cell adhesion, M cells showed higher adhesion forces than Caco-2 cells. The combination of distinct experimental techniques shows that morphological differences between Caco-2 cells and M cells correlate with mechanical cell properties and provide useful information to understand physiological processes/mechanisms in the small intestine.

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Section: Resultsmentioning

confidence: 99%

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

Schimpel

Werzer

Frőhlich

et al. 2015

Beilstein J. Nanotechnol.

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“…Table 1. Cellular fatty acid composition of strain 2-3 T and its phylogenetically closest relatives Strains: 1, 2-3 T ; 2, Pacificibacter maritimus KMM 9031 T (data from Romanenko et al 2011a); 3, Celeribacter neptunius H14 T (Ivanova et al, 2010); 4 Vadicella arenosi KMM 9024 T (Romanenko et al, 2011b). Values are percentages of the total fatty acids and are shown when present at ¢0.5 % in at least one strain.…”

Section: Tropicibacter Naphthalenivorans C02 T (Ab302370)mentioning

confidence: 99%

“…Table 2. Selected differential characteristics between strain 2-3 T and its phylogenetically closest relatives in the family Rhodobacteraceae Strains: 1, 2-3 T ; 2, P. maritimus KMM 9031 T (data from Romanenko et al 2011a); 3, C. neptunius H14 T (Ivanova et al, 2010); 4, V. arenosi KMM 9024 T (Romanenko et al, 2011b). +, Positive; 2, negative; ND, no data available; L, unidentified lipid; AL, unidentified aminolipid; PL, unidentified phospholipid; PA, phosphatidic acid.…”

Section: Tropicibacter Naphthalenivorans C02 T (Ab302370)mentioning

confidence: 99%

Amylibacter marinus gen. nov., sp. nov., isolated from surface seawater

Teramoto

Nishijima

2014

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, non-motile, mesophilic, aerobic, rod-shaped bacterium, designated strain 2-3 T , was isolated from surface seawater at Muroto city, Kochi prefecture, Japan. This strain grew well with starch. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the family Rhodobacteraceae and that the strain was related most closely to the genus Pacificibacter (94.0 % sequence similarity to the type strain). The DNA G+C content was 52.4 mol%. The major fatty acids were C 18 : 1 v7c, C 14 : 0 and C 16 : 0 . The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified lipid, one unidentified aminolipid and one unidentified phospholipid. The major isoprenoid quinone was Q-10. Strain 2-3 T did not grow at 4 or 35 6C, while the type strain of the type species of the genus Pacificibacter grows at both temperatures. From the taxonomic data obtained in this study, it is proposed that strain 2-3 T be placed into a novel genus and species named Amylibacter marinus gen. nov., sp. nov. in the family Rhodobacteraceae. The type strain of Amylibacter marinus is 2-3 T (5NBRC 110140 T 5LMG 28364 T ).Muroto city, Kochi prefecture, Japan is one of the rare areas where deep seawater is upwelling. Deep seawater is relatively rich in nitrogen and phosphorus (DeLong et al., 2006;Hansman et al., 2009), which are the two main growth-limiting factors in marine environments. Therefore, surface seawater around Muroto could potentially be more nutrient-rich than surface seawater in other areas, suggesting that rich microbial resources might be harboured in the Muroto surface seawater. The present study describes a novel bacterium, designated strain 2-3 T , isolated from Muroto surface seawater (33 u 189 N 134 u 119 E, water depth of 0.5 m).With regard to the original habitat, strain 2-3 T may prefer relatively nutrient-rich marine environments. Specifically, 1 of 94 16S rRNA gene fragments obtained from the surface of a fish living in Muroto deep seawater displayed 98.8 % similarity to that of strain 2-3 T . However, such a similar fragment (with 97 % or more similarity) has not been found out of 7746 fragments obtained from the surrounding Muroto deep seawater (M. Teramoto and others, unpublished data).Strain 2-3 T was obtained from seawater by direct plating on DSW2 plates, which comprised (per litre deep seawater from the coastal area of Muroto; 33 u 189 N 134 u 149 E, depth of 320 m) 15 g agar, 0

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“…The genus Celeribacter , a member of the family Rhodobacteraceae , was first proposed by Ivanova et al (2010). Following reclassification of Huaishuia halophilus as a member of the genus Celeribacter by Lai et al (2014), this genus comprised five species at the time of writing: Celeribacter neptunius (Ivanova et al , 2010), Celeribacter baekdonensis (Lee et al , 2012), Celeribacter halophilus (Wang et al , 2012), Celeribacter marinus (Baek et al , 2014) and Celeribacter indicus (Lai et al , 2014). While the first four species were isolated from coastal surface seawater, C. indicus was isolated from deep-sea sediment.…”

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confidence: 99%

Celeribacter manganoxidans sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediment of a polymetallic nodule province

Wang

Liu

Wang

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped, manganese-oxidizing bacterial strain, designated DY2-5 T , was isolated from surface sediment of Pacific Clarion-ClippertonFracture Zone (CCFZ). Growth occurred at 0-37 8C (optimum 28 8C), pH 6.5-9.0 (optimum pH 7.0-7.5) and in the presence of 1-11 % (w/v) NaCl (optimum 3-4 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel strain was most closely related to Celeribacter halophilus ZXM137 T with 96.13 % sequence similarity, and had 16S

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Celeribacter neptunius gen. nov., sp. nov., a new member of the class Alphaproteobacteria

Cited by 29 publications

References 34 publications

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

Amylibacter marinus gen. nov., sp. nov., isolated from surface seawater

Celeribacter manganoxidans sp. nov., a manganese-oxidizing bacterium isolated from deep-sea sediment of a polymetallic nodule province

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