Cefsulodin Penetration into Rat Brain: Extracellular versus Total Concentration

Abstract

Disposition of cefsulodin (125 mg · kg^––¹) was studied in rat frontal cortex after intravenous injection of a bolus by two methods. In vivo voltametry and high performance liquid chromatography of cefsulodin in brain extracts gave different concentration values. The ratio of concentrations determined by the two methods was similar to the ratio of the extravascular volume to total volume. Thus, these findings evidenced the distribution of cefsulodin in the extravascular fluid of the rat frontal cortex.

Measurement and clinical and pharmacokinetic implications of diffusion coefficients of antibiotics in tissues

“…(26) and the first term was neglected. The parent drug was detected in its free form in the extracellular brain space (16,20,21). This fact was due to the fine tip of the microelectrodes, which were identical to oxygen microelectrodes used in brain physiology studies (17) (24,26,27).…”

“…The width of 150 to 160 mV. The reproducibility of concentration determinations was less than 5% (16)(17)(18)(19)(20)(21)(22)29). Under the described conditions, the limit of sensitivity was equal to 10 pg-ml-' for the four compounds.…”

“…CeFfsulodin, jike ampicillin, is poorly bound to proteins. For bolus injections, the mathematical model is not opsies by high-pressure liquid chromatography and direct on-line determination with our microelectrodes (16,20). From a pharmacokinetical standpoint, tissue extracellular concentrations can be analyzed with a classical compartmental model (lag times are small).…”

Pharmacokinetics of Cefsulodin in Rat Cerebrospinal Fluid during Experimental <i>Pseudomonas aeruginosa </i>Meningitis

“…Cefsulodin was used at a dose (20 mg/kg) which can be in creased without problem (up to 100 mg/ kg). The fact that we observed a high brain tissue concentration in healthy animals might be an important point in P. aerugi nosa meningitis [9].…”

A model of cefoperazone tissue penetration: diffusion coefficient and protein binding

“…This technique, called voltammetry, has been described many times (8,11). Briefly, a microelectrode of 1-,um diameter detects electroactive antibiotic concentrations without delay.…”