Cefquinome-loaded microsphere formulations against<i>Klebsiella pneumonia</i>infection during experimental infections

Qu, Shaoqi; Dai, Cunchun; Zhu, Jiajia; Zhao, Li; Li, Yuwen; Hao, Zhihui

doi:10.1080/10717544.2018.1461958

Cited by 9 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We carried out research on extracting protocatechuic aldehyde from the aerial part of S. miltiorrhiza, and explored its main medicinal effects. Although the effects of polysaccharides and some TCM monomers on inflammatory factors have been studied, the effects of PAI on inflammatory factors remain unknown [ 20 , 21 ]. In the present study, we found that the administration of PAI improved the survival rate of mice and cells and attenuated the LPS induced acute phase response in an LPS mice model.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway

Wang²,

Wang

et al. 2020

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

Background The aerial parts of Salvia miltiorrhiza, which was considered to be the waste part and discarded during the root harvest, is rich in protocatechuic aldehyde (PAI). This study investigated the health-promoting effects of extracts and PAI from the aerial parts of Salvia miltiorrhiza, including its anti-inflammatory effects and the underlying mechanisms of action in vitro and in vivo. Method Purification of the sample paste of Salvia miltiorrhiza was accomplished using HPLC analysis. TheMTT (Methylthiazolyldiphenyl-tetrazolium bromide) assay was employed to determine the cell viability. The production of inflammatory factors was detected by ELISA assays. The histopathological analysis was used to analyse the lungs and livers of mice treated with PAI. Western blot was performed to reveal the mechanism of PAI in anti-inflammatory. Results The extracts and PAI from the aerial parts of Salvia miltiorrhiza inhibited TNF-α, IL-6 production and promoted the production of IL-10 in vivo in mice and in vitro in the macrophage cell line RAW264.7. NF-κB and MAPKs kinase phosphorylation were also suppressed by PAI in vivo and in vitro, indicating that PAI exhibited an anti-inflammatory effect. Conclusion These findings suggest that the aerial parts of Salvia miltiorrhiza extract may serve as potential protective agents for inflammatory.

show abstract

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway

Wang²,

Wang

et al. 2020

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

show abstract

“…Measurement of caspase-9 and -3 activities and TNF-␣ and IL-1␤ levels. The supernatants (stored at -80°C) were used to determine the activities of caspase-3 and caspase-9 (Nanjing Jian Cheng Biotechnology Co., Ltd., Nanjing, China) and TNF-␣ and IL-1␤ (R&D Systems, Minneapolis, MN) using commercial ELISA kits according to manufacturers' instructions (51).…”

Section: Methodsmentioning

confidence: 99%

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Dai

Lang

et al. 2019

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

Nephrotoxicity is the major limiting factor for the clinical use of vancomycin (VCM) for treatment of serious infections caused by multiresistant Gram-positive bacteria. This study investigated the renal protective activity of rutin in a rat model of VCM-induced kidney injury in male Wistar rats. VCM administered intraperitoneally at 200 mg/kg twice daily for 7 successive days resulted in significant elevation of blood urea nitrogen and creatinine, as well as urinary N-acetyl-β-D-glucosaminidase. Coadministration of VCM with oral rutin at 150 mg/kg significantly reduced these markers of kidney damage. Rutin also significantly attenuated VCM-induced oxidative stress, inflammatory cell infiltration, apoptosis, and decreased interleukin-1β and tumor necrosis factor alpha levels (all P < 0.05 or 0.01) in kidneys. Renal recovery from VCM injury was achieved by rutin through increases in Nrf2 and HO-1 and a decrease in NF-κB expression. Our results demonstrated a protective effect of rutin on VCM-induced kidney injury through suppression of oxidative stress, apoptosis, and downregulation of the inflammatory response. This study highlights a role for oral rutin as an effective intervention to ameliorate nephrotoxicity in patients undergoing VCM therapy.

show abstract

“…We obtained PLGA microspheres of different particle sizes (3 mm: PLGA-3; 10 mm: PLGA-10; 25 mm: PLGA-25; 40 mm: PLGA-40,) through improving a method described by Qu et al (Fig. S1) [5]. To obtain the optimal particle size for lung-targeting, we performed imaging experiments for lung blood vessels using micro CT (Fig.…”

Section: Effect Of Microspheres Sizes On the Lung-targetingmentioning

confidence: 99%

“…Administration to the lungs through the capillaries for innovative formulation is a promising method [2]. Passive lung-targeting microspheres based on microembolism can directly reach the lungs, allowing localized precision treatment of the respiratory system disease, and greatly improving drug bioavailability [5,6,7]. Studies have proven that biodegradable microspheres have achieved effective pulmonary delivery of drug and treatment of pulmonary diseases in animal models [8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Effect of Sizes on the Lung-Targeting and Biocompatibility of Intravenously Administered Biodegradable PLGA Microspheres

Lang

Kang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: In the treatment of lung diseases, drug showed low bioavailability and efficacy by conventional administration methods. Passive lung-targeting microspheres provide a method to deliver drugs from the vascular side, there is still a paucity of systematic studies on the biocompatibility of biodegradable-polymer microspheres. Results: We used poly (lactic acid-glycolic acid) (PLGA) microspheres with different particle sizes (3, 10, 25, and 40 mm) as a model. The optimal lung-targeting particle size of the PLGA microspheres is 10 mm and the corresponding range of maximum tolerated dose is 125-150 mg/kg. We hypothesized that the decrease of blood oxygen saturation under treatment of microspheres was caused by pulmonary embolism. We found varying degrees of blood circulation loss of lungs by micro computed tomography test, which indicated severe pulmonary embolism subsequent to intravenous injection of microspheres with a particle size >25 mm. Furthermore, we found lung injury and microspheres leaked from the blood vessels into the alveoli by H&E staining. This process was likely induced by increased secretion of matrix metalloproteinases (MMPs), which destroyed the alveolar-capillary barrier and trigger an inflammatory cascade. Finally, we found that optimal particle size and dose conditions did not affect normal physiological activities after the microspheres degraded. The upregulation of vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecule-1 (PECAM-1 or CD31) induced vessel recanalization and reestablishment, which promoted the progress of lung repair. Conclusions: Collectively, our results highlight the importance of accurately designing the optimal size and dose of microspheres for passive lung-targeting delivery.

show abstract

Cefquinome-loaded microsphere formulations againstKlebsiella pneumoniainfection during experimental infections

Cited by 9 publications

References 38 publications

RETRACTED ARTICLE: Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway

RETRACTED ARTICLE: Protocatechuic aldehyde from Salvia miltiorrhiza exhibits an anti-inflammatory effect through inhibiting MAPK signalling pathway

Rutin Attenuates Vancomycin-Induced Nephrotoxicity by Ameliorating Oxidative Stress, Apoptosis, and Inflammation in Rats

Effect of Sizes on the Lung-Targeting and Biocompatibility of Intravenously Administered Biodegradable PLGA Microspheres

Contact Info

Product

Resources

About