Cefotaxime-resistant Citrobacter freundii in isolates from blood in a tertiary teaching hospital in Northern Taiwan

Liu, Chang-Pan; Weng, Li-Chuan; Tseng, Hsiang-Kuang; Wang, Naiyu; Lee, Chun-Ming

doi:10.1016/j.jinf.2007.04.010

Cited by 12 publications

(10 citation statements)

References 21 publications

(17 reference statements)

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“…CPS was better at detecting both of these. [12,13]. Reports on drug resistance in Serratia spp., however, are scanty.…”

Section: Discussionmentioning

confidence: 99%

“…The strains were genetically unrelated as determined by their sensitivity profiles, which differed from each other. These isolates were obtained from surgical site infections (57), orthopaedic infections (36), urinary tract infections (28), cerebrospinal fluid (5), sputum (11), and ear and eye samples (12). Isolates were identified by standard biochemical techniques [8].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Extended spectrum AmpC and metallo-beta-lactamases in Serratia and Citrobacter spp. in a disc approximation assay

Rizvi

Fatima

Rashid

et al. 2009

J Infect Dev Ctries

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Objectives: This study aimed to develop a novel model for detection of extended spectrum beta-lactamase (ESBL), AmpC and metallo-betalactamase (MBL) producing Serratia and Citrobacter species using cefoperazone sulbactam as well as other inducer-substrate combinations in a disc approximation assay. In the absence of molecular tools in developing countries, we attempted to standardize simple phenotypic techniques for detection of beta-lactamases to allow effective patient care in our countries. These techniques have been scarcely used in Serratia and Citrobacter spp., which are emerging as significant pathogens in our region. Methodology: Clinical isolates of Serratia and Citrobacter were tested for ESBL production. Cefoperazone (CP)/cefoperazone sulbactam (CPS), piperacillin (PIP)/piperacillin-tazobactam (TZP) and ceftazidime (CAZ)/ceftazidime-clavulanic acid (CAZ-CLAV) combinations were compared for their ability to detect ESBL producers phenotypically. Multi-drug resistant strains were further tested for detection of inducible/derepressed AmpC mutants by a disc approximation assay. Isolates were screened for MBL production by Imipenem (IMI). MBL production was confirmed using Ethylenediaminetetraacetic acid (EDTA) in a double disc synergy assay and Hodge test. Minimal inhibitory concentration (MIC) was performed for CP, CPS and IMI by agar dilution method for all isolates of Serratia and Citrobacter spp. Results: Thirty-three percent of isolates of Serratia spp. and 35.4% of Citrobacter spp. were ESBL producers. CPS was a more sensitive inducer of ESBL than TZP and CAZ/CAZ-CLAV. AmpC producers were detected in 25.6% of the isolates of Serratia spp. (40% inducible and 60% derepressed mutants) and in 35.4% of the isolates of Citrobacter spp. (33% inducible and 66% derepressed mutants). Six isolates (four class B and two class A) of Serratia and eight isolates (seven class B and one class A) of Citrobacter spp. were MBL producers. Multiple mechanisms co-existed in eight isolates of Serratia and 15 isolates of Citrobacter spp. CPS was more effective in identifying ESBLs and inducible AmpC producers as well as type 1 carbapenemases than TZP and CAZ-CLAV were able to identify inducible AmpC producers. Conclusions: The high prevalence of ESBL, AmpC, and MBL in Serratia and Citrobacter species in this study suggests that detection of these by phenotypic methods in the absence of more specific molecular tests should be actively considered in not only developing countries but also in the developed world as this approach can lead to timely and appropriate antibiotic treatment. CPS may be advised due to the triple advantage of detection of all three types of beta-lactamases.

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“…CPS was better at detecting both of these. [12,13]. Reports on drug resistance in Serratia spp., however, are scanty.…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Extended spectrum AmpC and metallo-beta-lactamases in Serratia and Citrobacter spp. in a disc approximation assay

Rizvi

Fatima

Rashid

et al. 2009

J Infect Dev Ctries

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“…A specific epidemic strain of A. baumannii causes infection or colonization of numerous patients. [21][22][23][24] Liu et al 25 reported that the bloodstream infections of the patients were caused by C. freundii. Citrobacter infections are often nosocomial, are commonly drug resistant, and have been seen and isolated in patients with significant underlying diseases.…”

Section: Pathogen/primer Dna Sequence (5-3) Referencementioning

confidence: 99%

“…38 Results of previous studies indicate that some specific bacteria isolated from patients caused nosocomial infections. [21][22][23][24][25][26][27][28][29][30][31]33 Thus, the activity of occupants may influence the presence of infectious bioaerosols in the environment. The fluctuation of bacterial flora in the air is also noted when patients and medical devices are transferred into and out of the room; it then remains steady but adaptable thereafter.…”

Section: Pathogen/primer Dna Sequence (5-3) Referencementioning

confidence: 99%

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Feng

Sung

et al. 2011

Journal of the Air & Waste Management Association

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Indoor air quality has become a critical issue because people spend most of their time in the indoor environment. The factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. This study focuses on monitoring air quality, colony counts, and bacteria species of the indoor air of a nursing care institution. The regular colony counts in two different wards range from 55 to 600 cfu m(-3) Regression analysis results indicate that the bacterial colony counts have close correlation with relative humidity or carbon dioxide (CO2) but not with carbon monoxide (CO) or ozone (O3). Real-time PCR was used to quantify the bacterial pathogens of nosocomial infection, including Acinetobacter baumannii, Citrobacter freundii, Escherichia coli, Klebsiella pneumoniae, and methicillin-sensitive Staphylococcus aureus. The most abundant bacteria species in the air of the nursing care institution is E. coli.

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“…Resistance to antibiotics may be caused by several mechanisms, but the most important of which is the production of β-lactamase that hydrolyzes the β-lactam ring (Lin et al, 2003;Liu et al, 2007). β-Lactamases (EC 3.5.2.6) are the primary agents of bacterial resistance to penicillins and cephalosporins.…”

Section: Introductionmentioning

confidence: 99%

Cefotaxime and ceftriaxon resistantKlebsiella pneumoniae associated with SHV-11 hyperproduction

Achour¹,

Mercuri

Belhadj³

et al. 2008

Ann. Microbiol.

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Klebsiella pneumoniae ML0306 was isolated from a patient in the intensive care unit of the Military hospital of Tunis in Tunisia. The strain was resistant to β-lactams, aminoglycosides, quinolones, phenicols and tetracyclines. Crude extract from the isolate showed a broad-substrate profile by hydrolyzing benzylpenicillin, ampicillin, cloxacilline, cephalothin, cefotaxime and ceftriaxone but not ceftazidime, cefoxitin, cefpirome, imipinem and aztreonam. The isolate was identified as a producer of pI 7.6 β-lactamase. Sequencing of the β-lactamase gene showed that it coded for a penicillinase SHV-11. This is the first description of this enzyme in Tunisia. The gene encoding this enzyme was located on the chromosome and a 50 kb conjugative plasmid. The overproduction of SHV-11 was involved in the wading of the hydrolytic spectrum of the strain.

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Cefotaxime-resistant Citrobacter freundii in isolates from blood in a tertiary teaching hospital in Northern Taiwan

Cited by 12 publications

References 21 publications

Extended spectrum AmpC and metallo-beta-lactamases in Serratia and Citrobacter spp. in a disc approximation assay

Extended spectrum AmpC and metallo-beta-lactamases in Serratia and Citrobacter spp. in a disc approximation assay

Quantification and Analysis of Airborne Bacterial Characteristics in a Nursing Care Institution

Cefotaxime and ceftriaxon resistantKlebsiella pneumoniae associated with SHV-11 hyperproduction

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