Cefotaxime incorporated bimetallic silver-selenium nanoparticles: promising antimicrobial synergism, antibiofilm activity, and bacterial membrane leakage reaction mechanism

Elakraa, Abdelrahman A.; Salem, Salem S.; El-Sayyad, Gharieb S.; Attia, Mohamed S.

doi:10.1039/d2ra04717a

Cited by 60 publications

(18 citation statements)

References 94 publications

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“…This disintegration of the membrane was affirmed by increasing in the leakage of DNA and proteins. These findings concord with previous reports, suggesting that the antimicrobial effect of various nanoparticles may be attributed to the disintegration of membranes and leakage of the intracellular content, as well as the rupture of cell walls [ 2 , 86 , 87 , 88 , 89 , 90 , 91 ]. Interestingly, the biogenic TeNPs produced by Gayadomonas sp.…”

Section: Discussionsupporting

confidence: 86%

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

et al. 2023

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The biosynthesis of nanoparticles using green technology is emerging as a cost-efficient, eco-friendly and risk-free strategy in nanotechnology. Recently, tellurium nanoparticles (TeNPs) have attracted growing attention due to their unique properties in biomedicine, electronics, and other industrial applications. The current investigation addresses the green synthesis of TeNPs using a newly isolated mangrove-associated bacterium, Gayadomonas sp. TNPM15, and their impact on the phytopathogenic fungi Fusarium oxysporum and Alternaria alternata. The biogenic TeNPs were characterized using transmission electron microscopy (TEM), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Raman spectroscopy and Fourier transform infrared (FTIR). The results of TEM revealed the intracellular biosynthesis of rod-shaped nanostructures with a diameter range from 15 to 23 nm and different lengths reaching up to 243 nm. Furthermore, the successful formation of tellurium nanorods was verified by SEM-EDX, and the XRD pattern revealed their crystallinity. In addition, the FTIR spectrum provided evidence for the presence of proteinaceous capping agents. The bioinspired TeNPs exhibited obvious inhibitory effect on the spores of both investigated phytopathogens accomplished with prominent ultrastructure alternations, as evidenced by TEM observations. The biogenic TeNPs impeded spore germination of F. oxysporum and A. alternata completely at 48.1 and 27.6 µg/mL, respectively. Furthermore, an increase in DNA and protein leakage was observed upon exposure of fungal spores to the biogenic TeNPs, indicating the disruption of membrane permeability and integrity. Besides their potent influence on fungal spores, the biogenic TeNPs demonstrated remarkable inhibitory effects on the production of various plant cell wall-degrading enzymes. Moreover, the cytotoxicity investigations revealed the biocompatibility of the as-prepared biogenic TeNPs and their low toxicity against the human skin fibroblast (HSF) cell line. The biogenic TeNPs showed no significant cytotoxic effect towards HSF cells at concentrations up to 80 μg/mL, with a half-maximal inhibitory concentration (IC50) value of 125 μg/mL. The present work spotlights the antifungal potential of the biogenic TeNPs produced by marine bacterium against phytopathogenic fungi as a promising candidate to combat fungal infections.

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Section: Discussionsupporting

confidence: 86%

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

et al. 2023

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“…Usually, physical, chemical, and biological processes may be used to create nanoparticles. In the physical and chemical processes, sodium borohydride and other strong-chemical reducing agent are utilized, whereas sodium citrate, alcohols, gamma- and UV radiation, among others, are the weak-chemical reducing agent (Elakraa et al 2022 ; Salem et al 2022a ; Abdelmoneim et al 2022 ). According to studies, producing nanoparticles via biological techniques is a cheap and environmentally benign process.…”

Section: Introductionmentioning

confidence: 99%

A mini review on green nanotechnology and its development in biological effects

Salem

2023

Arch Microbiol

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The utilization of living organisms for the creation of inorganic nanoscale particles is a potential new development in the realm of biotechnology. An essential milestone in the realm of nanotechnology is the process of creating dependable and environmentally acceptable metallic nanoparticles. Due to its increasing popularity and ease, use of ambient biological resources is quickly becoming more significant in this field of study. The phrase “green nanotechnology” has gained a lot of attention and refers to a variety of procedures that eliminate or do away with hazardous compounds to repair the environment. Green nanomaterials can be used in a variety of biotechnological sectors such as medicine and biology, as well as in the food and textile industries, wastewater treatment and agriculture field. The construction of an updated level of knowledge with utilization and a study of the ambient biological systems that might support and revolutionize the creation of nanoparticles (NPs) are presented in this article.

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“…Silver nanoparticles (Ag-NPs) have been considered for use in sensors, cosmetics, paint/varnishes, and biomedical products due to their unusual optical, electronic, and catalytic properties (Al-Rajhi et al, 2022;Salem and Fouda, 2021;Salem et al, 2022a,b;Elakraa et al, 2022). Their unique physicochemical properties have made them one of the most commercialized nanomaterials (Chen and Schluesener, 2008).…”

Section: Introductionmentioning

confidence: 99%

A whole cell fluorescence quenching-based approach for the investigation of polyethyleneimine functionalized silver nanoparticles interaction with Candida albicans

Tiwari

Gupta²,

Narayan³

et al. 2023

Front. Microbiol.

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The antimicrobial activity of metal nanoparticles can be considered a two-step process. In the first step, nanoparticles interact with the cell surface; the second step involves the implementation of the microbicidal processes. Silver nanoparticles have been widely explored for their antimicrobial activity against many pathogens. The interaction dynamics of functionalized silver nanoparticles at the biological interface must be better understood to develop surface-tuned biocompatible nanomaterial-containing formulations with selective antimicrobial activity. Herein, this study used the intrinsic fluorescence of whole C. albicans cells as a molecular probe to understand the cell surface interaction dynamics of polyethyleneimine-functionalized silver nanoparticles and antifungal mechanism of the same. The results demonstrated that synthesized PEI-f-Ag-NPs were ~ 5.6 ± 1.2 nm in size and exhibited a crystalline structure. Furthermore, the recorded zeta potential (+18.2 mV) was associated with the stability of NPS and shown a strong electrostatic interaction tendency between the negatively charged cell surface. Thus, rapid killing kinetics was observed, with a remarkably low MIC value of 5 μg/mL. PEI-f-Ag-NPs quenched the intrinsic fluorescence of C. albicans cells with increasing incubation time and concentration and have shown saturation effect within 120 min. The calculated binding constant (Kb = 1 × 105 M−1, n = 1.01) indicated strong binding tendency of PEI-f-Ag-NPs with C. albicans surface. It should also be noted that the silver nanoparticles interacted more selectively with the tyrosine-rich proteins in the fungal cell. However, calcofluor white fluorescence quenching showed non-specific binding on the cell surface. Thus, the antifungal mechanisms of PEI-f-Ag-NPs were observed as reactive oxygen species (ROS) overproduction and cell wall pit formation. This study demonstrated the utility of fluorescence spectroscopy for qualitative analysis of polyethyleneimine-functionalized silver nanoparticle interaction/binding with C. albicans cell surface biomolecules. Although, a quantitative approach is needed to better understand the interaction dynamics in order to formulate selective surface tuned nanoparticle for selective antifungal activity.

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Cefotaxime incorporated bimetallic silver-selenium nanoparticles: promising antimicrobial synergism, antibiofilm activity, and bacterial membrane leakage reaction mechanism

Abstract: In this research, we reported for the first time the simple incorporation of antibiotic cefotaxime (CFM) with Ag NPs, Se NPs, and bimetallic Ag–Se NPs by gamma rays; as a promising cost-effective, and eco-friendly method.

Cited by 60 publications

References 94 publications

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

Biosynthesis of Novel Tellurium Nanorods by Gayadomonas sp. TNPM15 Isolated from Mangrove Sediments and Assessment of Their Impact on Spore Germination and Ultrastructure of Phytopathogenic Fungi

A mini review on green nanotechnology and its development in biological effects

A whole cell fluorescence quenching-based approach for the investigation of polyethyleneimine functionalized silver nanoparticles interaction with Candida albicans

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