Cefoperazone sodium liposomal formulation to mitigate P. aeruginosa biofilm in Cystic fibrosis infection: A QbD approach

Ghodake, Vinod; Vishwakarma, Jyoti; Vavilala, Sirisha L.; Patravale, Vandana

doi:10.1016/j.ijpharm.2020.119696

Cited by 18 publications

(5 citation statements)

References 34 publications

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“…Further, unadhered cells were washed using distilled water and biofilms in the wells were quantified using the crystal violet assay as mentioned above. The assay was performed thrice, and percentage eradication was graphically represented against Ts-AgNPs’ concentration [ 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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Several microbial pathogens are capable of forming biofilms. These microbial communities pose a serious challenge to the healthcare sector as they are quite difficult to combat. Given the challenges associated with the antibiotic-based management of biofilms, the research focus has now been shifted towards finding alternate treatment strategies that can replace or complement the antibacterial properties of antibiotics. The field of nanotechnology offers several novel and revolutionary approaches to eradicate biofilm-forming microbes. In this study, we evaluated the antibacterial and antibiofilm efficacy of in-house synthesized, tryptone-stabilized silver nanoparticles (Ts-AgNPs) against the superbug Serratia marcescens . The nanoparticles were of spherical morphology with an average hydrodynamic diameter of 170 nm and considerable colloidal stability with a Zeta potential of − 24 ± 6.15 mV. Ts-AgNPs showed strong antibacterial activities with a minimum inhibitory concentration (MIC 50 ) of 2.5 µg/mL and minimum bactericidal concentration (MBC) of 12.5 µg/mL against S. marcescens . The nanoparticles altered the cell surface hydrophobicity and inhibited biofilm formation. The Ts-AgNPs were also effective in distorting pre-existing biofilms by degrading the extracellular DNA (eDNA) component of the extracellular polymeric substance (EPS) layer. Furthermore, reduction in quorum-sensing (QS)-induced virulence factors produced by S. marcescens indicated that Ts-AgNPs attenuated the QS pathway. Together, these findings suggest that Ts-AgNPs are an important anti-planktonic and antibiofilm agent that can be explored for both the prevention and treatment of infections caused by S. marcescens . Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00775-022-01977-w.

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Section: Methodsmentioning

confidence: 99%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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“…Because of this, SSD egg oil organogel holds promise as a delivery strategy for the treatment of burn wounds and associated infections. Systematic QbD was utilized by Ghodake et al [ 77 ] to create a dry powder inhaler of sodium cefoperazone ( 1 , Figure 9 ) based on liposomes. The effectiveness of liposomes as an anti-biofilm agent against P. aeruginosa was examined.…”

Section: Novel Therapeutic Agentsmentioning

confidence: 99%

Design and Synthesis of Novel Antimicrobial Agents

Breijyeh

2023

Antibiotics

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The necessity for the discovery of innovative antimicrobials to treat life-threatening diseases has increased as multidrug-resistant bacteria has spread. Due to antibiotics’ availability over the counter in many nations, antibiotic resistance is linked to overuse, abuse, and misuse of these drugs. The World Health Organization (WHO) recognized 12 families of bacteria that present the greatest harm to human health, where options of antibiotic therapy are extremely limited. Therefore, this paper reviews possible new ways for the development of novel classes of antibiotics for which there is no pre-existing resistance in human bacterial pathogens. By utilizing research and technology such as nanotechnology and computational methods (such as in silico and Fragment-based drug design (FBDD)), there has been an improvement in antimicrobial actions and selectivity with target sites. Moreover, there are antibiotic alternatives, such as antimicrobial peptides, essential oils, anti-Quorum sensing agents, darobactins, vitamin B6, bacteriophages, odilorhabdins, 18β-glycyrrhetinic acid, and cannabinoids. Additionally, drug repurposing (such as with ticagrelor, mitomycin C, auranofin, pentamidine, and zidovudine) and synthesis of novel antibacterial agents (including lactones, piperidinol, sugar-based bactericides, isoxazole, carbazole, pyrimidine, and pyrazole derivatives) represent novel approaches to treating infectious diseases. Nonetheless, prodrugs (e.g., siderophores) have recently shown to be an excellent platform to design a new generation of antimicrobial agents with better efficacy against multidrug-resistant bacteria. Ultimately, to combat resistant bacteria and to stop the spread of resistant illnesses, regulations and public education regarding the use of antibiotics in hospitals and the agricultural sector should be combined with research and technological advancements.

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“…The efficacy of a variety of liposomal formulations against P. aeruginosa biofilms has been reported in the literature. Conventional charged and neutral liposomes have been observed to possess marked antibiofilm efficacy against P. aeruginosa [36,78,81,83,84]. In their study, Ibaraki and coworkers [81] investigate the anti-biofilm potential of surface modification by testing charged liposomes without or with polyethylene glycol (PEG) modification.…”

Section: Pseudomonas Aeruginosa Biofilmsmentioning

confidence: 99%

“…Another work reports the efficacy of conventional but neutral liposomal antibiotic formulations. Moreover, the study investigated encapsulating the antibiotic cefoperazone within conventional liposomal formulations and reported a significant reduction of its biofilm inhibitory concentration by half [83].…”

Section: Pseudomonas Aeruginosa Biofilmsmentioning

confidence: 99%

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Liposomes-Based Drug Delivery Systems of Anti-Biofilm Agents to Combat Bacterial Biofilm Formation

2023

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All currently approved antibiotics are being met by some degree of resistance by the bacteria they target. Biofilm formation is one of the crucial enablers of bacterial resistance, making it an important bacterial process to target for overcoming antibiotic resistance. Accordingly, several drug delivery systems that target biofilm formation have been developed. One of these systems is based on lipid-based nanocarriers (liposomes), which have shown strong efficacy against biofilms of bacterial pathogens. Liposomes come in various types, namely conventional (charged or neutral), stimuli-responsive, deformable, targeted, and stealth. This paper reviews studies employing liposomal formulations against biofilms of medically salient gram-negative and gram-positive bacterial species reported recently. When it comes to gram-negative species, liposomal formulations of various types were reported to be efficacious against Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, and members of the genera Klebsiella, Salmonella, Aeromonas, Serratia, Porphyromonas, and Prevotella. A range of liposomal formulations were also effective against gram-positive biofilms, including mostly biofilms of Staphylococcal strains, namely Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus subspecies bovis, followed by Streptococcal strains (pneumonia, oralis, and mutans), Cutibacterium acnes, Bacillus subtilis, Mycobacterium avium, Mycobacterium avium subsp. hominissuis, Mycobacterium abscessus, and Listeria monocytogenes biofilms. This review outlines the benefits and limitations of using liposomal formulations as means to combat different multidrug-resistant bacteria, urging the investigation of the effects of bacterial gram-stain on liposomal efficiency and the inclusion of pathogenic bacterial strains previously unstudied.

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Cefoperazone sodium liposomal formulation to mitigate P. aeruginosa biofilm in Cystic fibrosis infection: A QbD approach

Cited by 18 publications

References 34 publications

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Design and Synthesis of Novel Antimicrobial Agents

Liposomes-Based Drug Delivery Systems of Anti-Biofilm Agents to Combat Bacterial Biofilm Formation

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