Cefiderocol Is an Effective Topical Monotherapy for Experimental Extensively Drug-Resistant Pseudomonas aeruginosa Keratitis

Genome sequence generated by hybrid Nanopore-Illumina assembly of an extensively drug-resistant Pseudomonas aeruginosa strain from a keratitis outbreak

“…It carries genes for Verona integron-mediated metallo-β-lactamase and the Guiana extended-spectrum β-lactamase which has not been formerly seen in the United States. This bacterium is highly resistant to standard of care antibiotics for P. aeruginosa keratitis (ciprofloxacin and tobramycin) with minimum inhibitory concentrations above 32 µg/mL ( 1 , 3 ). Here, we report the genome of an isolate from this outbreak using whole genome sequencing (WGS) made using Illumina and Nanopore and assembled with Unicycler software.…”

“…MIC data are reported on the CDC’s website that describes antibiotic susceptibility for this isolate. Here, we investigate isolate “AR-1270,” [also noted as CDC1270 ( 1 , 3 )]. The CDC assigned BioSample ID SAMN33435243 to this isolate.…”