CEF1/OsMYB103L is involved in GA-mediated regulation of secondary wall biosynthesis in rice

Ye, Yafeng; Liu, Binmei; Zhao, Meng; Wu, Kun; Cheng, Weimin; Chen, Xiangbin; Liu, Qian; Liu, Zan; Fu, Xiang‐Dong; Wu, Yuejin

doi:10.1007/s11103-015-0376-0

Cited by 58 publications

(63 citation statements)

References 62 publications

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“…OsMYB46 and ZmMYB46 , the orthologues of Arabidopsis MYB46 / MYB83 , share similar functions and are able to activate the secondary wall biosynthetic program when overexpressed in Arabidopsis [14]. CEF1/OsMYB103L and MYB61 are also involved secondary wall biosynthesis mediated by the GA pathway, which can affect leaf shape, cellulose synthesis and mechanical strength in rice [15, 16]. …”

Section: Introductionmentioning

confidence: 99%

OsMPH1 regulates plant height and improves grain yield in rice

Zhang

Lin

et al. 2017

PLoS ONE

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Plant height is a major trait affecting yield potential in rice. Using a large-scale hybrid transcription factor approach, we identified the novel MYB-like transcription factor OsMPH1 (MYB-like gene of Plant Height 1), which is involved in the regulation of plant height in rice. Overexpression of OsMPH1 leads to increases of plant height and grain yield in rice, while knockdown of OsMPH1 leads to the opposite phenotypes. Microscopy of longitudinal stem sections indicated that a change in internode cell length resulted in the change in plant height. RNA sequencing (RNA-seq) analysis of transgenic rice lines showed that multiple genes related to cell elongation and cell wall synthesis, which are associated with plant height and yield phenotypes, exhibited an altered expression profile. These results imply that OsMPH1 might be involved in specific recognition and signal transduction processes related to plant height and yield formation, providing further insights into the mechanisms underlying the regulation of plant height and providing a candidate gene for the efficient improvement of rice yield.

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Section: Introductionmentioning

confidence: 99%

OsMPH1 regulates plant height and improves grain yield in rice

Zhang

Lin

et al. 2017

PLoS ONE

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“…AtMYB103 has been suggested as a key gene regulating the expression of ferulate-5-hydroxylase (F5H), which directs lignin biosynthesis towards formation of syringyl-rich lignin (Öhman et al ., 2013) and was initially suggested to regulate lignin composition exclusively. However, MYB103 orthologues of grass species have also been shown to promote cellulose and hemicellulose genes (Hirano et al ., 2013; Yang et al ., 2014; Ye et al ., 2015) and AtMYB103 at least associates with cellulose synthase promoter sequences (Zhong et al ., 2008; Taylor-Teeples et al ., 2015). The predicted MsMYB103 protein shares 43.9% identity and 51.9% similarity on amino acid level with AtMYB103 (Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It has been suggested that AtMYB103 specifically regulates F5H expression to modulate lignin S:G ratio (Öhman et al ., 2013). However, this direct and specific link could not be established for MYB103 proteins originating from grass species, which were shown to regulate cellulose and hemicellulose (Hirano et al ., 2013; Yang et al ., 2014; Ye et al ., 2015). In line with these results, MsMYB103 seems to orchestrate and finetune SCW formation and lignin biosynthesis more broadly.…”

Section: Resultsmentioning

confidence: 99%

“…For example, MYB103 in Arabidopsis has been described to specifically affect lignin composition without affecting total lignin and cellulose content (Öhman et al ., 2013). However, MYB103 orthologues from grass species seem to regulate a much broader spectrum of SCW-related target genes (Hirano et al ., 2013; Yang et al ., 2014; Ye et al ., 2015). Thus, while the SCW transcriptional network seems to be conserved to some degree, recent evidence suggests grass-specific nuances exist (Rao and Dixon, 2018), in line with an expansion of the MYB class of TFs in monocots (Rabinowicz et al ., 1999; Zhao and Bartley, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Distinct and overlapping functions ofMiscanthus sinensisMYB transcription factors SCM1 and MYB103 in lignin biosynthesis

Golfier

Unda

Murphy

et al. 2019

Preprint

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38 Cell wall recalcitrance is a major constraint for the exploitation of lignocellulosic biomass as 39 renewable resource for energy and bio-based products. Transcriptional regulators of the lignin 40 biosynthetic pathway represent promising targets for tailoring lignin content and composition in 41 plant secondary cell walls. A wealth of research in model organisms has revealed that 42 transcriptional regulation of secondary cell wall formation is orchestrated by a hierarchical 43 transcription factor (TF) network with NAC TFs as master regulators and MYB factors in the lower 44 tier regulators. However, knowledge about the transcriptional regulation of lignin biosynthesis in 45 lignocellulosic feedstocks, such as Miscanthus, is limited. Here, we characterized two Miscanthus 46 MYB TFs, MsSCM1 and MsMYB103, and compared their transcriptional impact with that of the 47 master regulator MsSND1. In Miscanthus leaves MsSCM1 and MsMYB103 are expressed at 48 growth stages associated with lignification. Ectopic expression of MsSCM1 and MsMYB103 in 49 tobacco leaves was sufficient to trigger secondary cell wall deposition with distinct sugar and lignin 50 composition. Moreover, RNA-seq analysis revealed that the transcriptional responses to MsSCM1 51 and MsMYB103 overexpression showed extensive overlap with the response to MsSND1, but 52 were distinct from each other, underscoring the inherent complexity of secondary cell wall 53 formation. Together, MsSCM1 and MsMYB103 represent interesting targets for manipulations of 54 lignin content and composition in Miscanthus towards tailored biomass. 55 56 157 158 Tissue Staining and Microscopy 159

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“…Brittle culm is an important constraint to high-yielding stable rice production. This mutation can lead to a decrease in the mechanical strength of stalks, thereby reducing the lodging resistance of rice [25,26]. In this study, we analysed a brittle culm mutant generated by ion-beam irradiation.…”

Section: Pcr and Hrm Analysis Of Rice Mutants On The Instrumentmentioning

confidence: 99%

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

Zhu

et al. 2019

Biotechnology & Biotechnological Equipment

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Rice is a crucial crop to meet global food demands, therefore, it is necessary to develop rapid, efficient and inexpensive methods for screening mutants of important genes in molecular breeding. In this research, a simple microfluidic chip-integrated one-step polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis were designed for the rapid screening of rice mutants. In order to complete the above processes, we also installed a self-developed functional instrument integrated with a temperature control system and a fluorescence detection department. This microfluidic platform was validated by analysing genes related to insect resistance, phenotype and yield of two important varieties of rice, Oryza sativa L. ssp. japonica. and Oryza sativa L. ssp. indica (93-11). The results showed that the platform could detect singlenucleotide mutations between the wild and the mutant rice plants in 1 h and the accuracy was consistent with gene sequencing regarded as the golden standard of the mutants screening. Compared to the traditional method (requiring approximately 3 h), this platform can distinguish mutants from wild types more rapidly. This system is a simple, integrated, disposable and lowcost tool for high-throughput screening of mutants in molecular breeding. More important, this portable instrument can be used as a point-of-care platform for nucleic acid diagnostics in many scientific fields. ARTICLE HISTORY

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CEF1/OsMYB103L is involved in GA-mediated regulation of secondary wall biosynthesis in rice

Cited by 58 publications

References 62 publications

OsMPH1 regulates plant height and improves grain yield in rice

OsMPH1 regulates plant height and improves grain yield in rice

Distinct and overlapping functions ofMiscanthus sinensisMYB transcription factors SCM1 and MYB103 in lignin biosynthesis

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

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