Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD

Zhai, Zhe; Zhang, Fan; Cao, Ruihua; Ni, Xiaojun; Xin, Zhongquan; Deng, Jinping; Wu, Guoyao; Ren, Wenkai; Yin, Yulong; Deng, Baichuan

doi:10.3389/fmicb.2019.01595

Cited by 87 publications

(72 citation statements)

References 46 publications

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“…These data are counterintuitive in the light of our data presented here and given that this family may be an indicator of colitis disease activity. In contrast, in the study by Zhai Z. et al (2019) , Marinifilaceae positively correlated with inflammatory status in the mice, which is in line with our study describing significant decreases in this family at d8 by CBD (1 mg/kg) + FO (20 mg/mouse), concomitant to decreased inflammation. Furthermore, both Marinifilaceae and Ruminococcaceae were decreased in rats fed an acorn-fed cured ham diet (having high levels of the mono-unsaturated fatty acid oleic acid), in conjunction with significant prevention of DSS-induced colitis symptoms ( Fernandez et al, 2020 ).…”

Section: Discussionsupporting

confidence: 91%

“…Indeed, DSS-induced increases of Desulfovibrionaceae in mice were abrogated through treatment with the probiotic Bifidobacterium breve ( Yang and Yang, 2018 ). However, Desulfovibrionaceae and Ruminococcaceae were both increased by gentamicin in DSS-treated mice in association with an improved DAI and inflammatory profile ( Zhai Z. et al, 2019 ). Most interestingly, Ptpn22 −/− mice, which are resistant to faster recovery in response to cohousing-mediated faecal microbiota transfer, have decreased Desulfovibrionaceae levels ( Spalinger et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Fish Oil, Cannabidiol and the Gut Microbiota: An Investigation in a Murine Model of Colitis

Silvestri

Pagano

Lacroix³

et al. 2020

Front. Pharmacol.

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Inflammatory bowel disorders can be associated with alterations in gut microbiota (dysbiosis) and behavioral disturbances. In experimental colitis, administration of fish oil (FO) or cannabinoids, such as cannabidiol (CBD), reduce inflammation. We investigated the effect of combined FO/CBD administration on inflammation and dysbiosis in the dextran sulphate sodium (DSS) model of mouse colitis, which also causes behavioral disturbances. Colitis was induced in CD1 mice by 4% w/v DSS in drinking water for five consecutive days followed by normal drinking water. FO (20–75 mg/mouse) was administered once a day starting two days after DSS, whereas CBD (0.3–30 mg/kg), alone or after FO administration, was administered once a day starting 3 days after DSS, until day 8 (d8) or day 14 (d14). Inflammation was assessed at d8 and d14 (resolution phase; RP) by measuring the Disease Activity Index (DAI) score, change in body weight, colon weight/length ratio, myeloperoxidase activity and colonic interleukin (IL)-1β (IL-1β), IL-10, and IL-6 concentrations. Intestinal permeability was measured with the fluorescein isothiocyanate-dextran. Behavioral tests (novel object recognition (NOR) and light/dark box test) were performed at d8. Fecal microbiota composition was determined by ribosomal 16S DNA sequencing of faecal pellets at d8 and d14. DSS-induced inflammation was stronger at d8 and accompanied by anxiety-like behavior and impaired recognition memory. FO (35, 50, 75 mg/mouse) alone reduced inflammation at d8, whereas CBD alone produced no effect at any of the doses tested; however, when CBD (3, 10 mg/kg) was co-administered with FO (75 mg/mouse) inflammation was attenuated. FO (20 mg/mouse) and CBD (1 mg/kg) were ineffective when given alone, but when co-administered reduced all inflammatory markers and the increased intestinal permeability at both d8 and d14, but not the behavioral impairments. FO, CBD, and their combination affected gut bacteria taxa that were not affected by DSS per se . Akkermansia muciniphila , a species suggested to afford anti-inflammatory action in colitis, was increased by DSS only at d14, but its levels were significantly elevated by all treatments at d8. FO and CBD co-administered at per se ineffective doses reduce colon inflammation, in a manner potentially strengthened by their independent elevation of Akkermansia muciniphila .

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Fish Oil, Cannabidiol and the Gut Microbiota: An Investigation in a Murine Model of Colitis

Silvestri

Pagano

Lacroix³

et al. 2020

Front. Pharmacol.

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“…A very recent report from Zhai et al. 32 suggested that cecropin A alleviates inflammation by modulating the gut microbiota in mice with inflammatory bowel disease. Haney et al.…”

Section: Discussionmentioning

confidence: 99%

The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis

Wang

Chen

et al. 2021

Acta Pharmaceutica Sinica B

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The antimicrobial peptide APKGVQGPNG (named YD), a natural peptide originating from Bacillus amyloliquefaciens CBSYD1, exhibited excellent antibacterial and antioxidant properties in vitro . These characteristics are closely related to inflammatory responses which is the central trigger for liver fibrosis. However, the therapeutic effects of YD against hepatic fibrosis and the underlying mechanisms are rarely studied. In this study, we show that YD improved liver function and inhibited the progression of liver fibrosis by measuring the serum transaminase activity and the expression of α -smooth muscle actin and collagen I in carbon tetrachloride-induced mice. Then we found that YD inhibited the level of miR-155, which plays an important role in inflammation and liver fibrosis. Bioinformatics analysis and luciferase reporter assay indicate that Casp12 is a new target of miR-155. We demonstrate that YD significantly decreases the contents of inflammatory cytokines and suppresses the NF- κ B signaling pathway. Further studies show that transfection of the miR-155 mimic in RAW264.7 cells partially reversed the YD-mediated CASP12 upregulation, the downregulated levels of inflammatory cytokines, and the inactivation of the NF- κ B pathways. Collectively, our study indicates that YD reduces inflammation through the miR-155– Casp12 –NF- κ B axis during liver fibrosis and provides a promising therapeutic candidate for hepatic fibrosis.

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“…Intestinal colitis was induced in all mice by administration of 3% DSS (MP Biomedicals Shanghai, Co., Ltd. molecular weight=165.192 g/mol) in drinking water for seven days as previously described 26 . After that, thirty mice with colitis were obtained and randomly assigned into three groups (n =8) including the control group (Control) without EA (EA, Sigma-Aldrich,CAS141-43-5) supplements and two treatment groups that were supplemented with 500μM EA (LowEA) and 3000 μM EA(HighEA) in drinking water for two weeks( Figure 1A).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Ethanolamine improves colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis

Zhou

Xiong

Wan

et al. 2020

Preprint

Self Cite

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Ethanolamine(EA) often occurs at a relatively high concentration within the inflamed gut of IBD patients. To investigate the role of EA in colonic inflammation and host-microbiome dysbiosis, thirty-six ICR mice were treated with 3% DSS for a week to generate acute intestinal inflammation and then supplied with 0 μM, 500 μM (LowEA), and 3000 μM (HighEA) in drinking water for two weeks, after that, 16s RNA sequencing was applied in characterizing the changes in colonic microbiota driven by different EA levels. An inflamed colonic organoid model via 3% DSS treatment was also established for further verification of these in vivo findings. EA significantly reduced proximal colonic crypt depth but increased distal colonic villus height in HighEA group. The protein and mRNA expression of occludin and Reg3β, BD1, BD2, and MUC2 were significantly up-regulated in EA treated groups. EA decreased mucosal inflammation-related cytokines levels (IL1, IL6, IL17, TNFα, and INFγ) and increased the significantly increased concentration of sIgA. Serum aspartate aminotransferase and alanine aminotransferase were significantly down-regulated in the highEA group. EA increased the relative abundance of Blautia, Roseburia, Lactobacillus, Faecalibaculum, Candidatus_Saccharimonas, Alloprevotella, and Lachnoclostridum and thus microbial metabolic pathways including Oxidative phosphorylation, Lipopolysaccharide biosynthesis, Arginine and proline metabolism, Folate biosynthesis, and Biotin metabolism were more abundant in LowEA group than those in control. EA up-regulated the protein or mRNA expression of TLR4/MyD88 in colonic tissues and the DSS-treated colonic organoid model. This study firstly demonstrated that ethanolamine in altering host-microbiome dysbiosis, which may provide new insights into the role of dietary lipids in IBD.

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Cecropin A Alleviates Inflammation Through Modulating the Gut Microbiota of C57BL/6 Mice With DSS-Induced IBD

Cited by 87 publications

References 46 publications

Fish Oil, Cannabidiol and the Gut Microbiota: An Investigation in a Murine Model of Colitis

Fish Oil, Cannabidiol and the Gut Microbiota: An Investigation in a Murine Model of Colitis

The antimicrobial peptide YD attenuates inflammation via miR-155 targeting CASP12 during liver fibrosis

Ethanolamine improves colonic barrier functions and inflammatory immunoreactions via shifting microbiome dysbiosis

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