Cecropia obtusifolia Bertol and its active compound, chlorogenic acid, stimulate 2-NBDglucose uptake in both insulin-sensitive and insulin-resistant 3T3 adipocytes

Alonso-Castro, Ángel Josabad; Miranda-Torres, Ana Cristina; González‐Chávez, Marco Martín; Salazar‐Olivo, Luis A.

doi:10.1016/j.jep.2008.09.019

Cited by 93 publications

(47 citation statements)

References 17 publications

(26 reference statements)

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“…Maximal response achieved by isolated SAB (concentration: 1 mg/ml) was comparable to glucose uptake activity of insulin alone (Figure 4). However, other studies have established that plant extracts and its compounds have the ability to stimulate glucose uptake far better in the presence of insulin than in the absence of insulin [39,40]. In contrast, our results demonstrate that, in the absence of insulin, magnesium salt of salvianolic acid B is equally efficacious in stimulating the glucose uptake in 3T3-L1 adipocytes, which suggests that SAB can increase glucose uptake through insulin independent mechanisms.…”

Section: Discussioncontrasting

confidence: 83%

Bioassay-guided Isolation of the Antidiabetic Active Principle from Salvia miltiorrhiza and its Stimulatory Effects on Glucose Uptake Using 3T3-L1 Adipocytes

Hu¹,

Amin²,

Kumar

et al. 2014

Med chem

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Section: Discussioncontrasting

confidence: 83%

Bioassay-guided Isolation of the Antidiabetic Active Principle from Salvia miltiorrhiza and its Stimulatory Effects on Glucose Uptake Using 3T3-L1 Adipocytes

Hu¹,

Amin²,

Kumar

et al. 2014

Med chem

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“…We previously showed that chlorogenic acid, an ester of caffeic acid, stimulated glucose uptake in insulin-sensitive and insulin-resistant 3T3-F442A adipocytes with a comparable potency with insulin or RGZ (28). We also showed that rexinoids honokiol and magnolol, isolated from Magnolia dealbata seeds, stimulated glucose uptake in insulin-sensitive and insulin-resistant murine and human adipocytes using the insulin signaling pathway (40).…”

Section: Discussionmentioning

confidence: 88%

“…Murine 3T3-F442A and human adipocytes were differentiated on 96-well fluorescence plates as described previously (28). Briefly, 3T3-F442A preadipocytes were induced to adipogenesis with DMEM containing 10% FBS, insulin 5 g/ml, and d-biotin 1 M, whereas human preadipocytes were differentiated into adipocytes with L15 medium containing 5% FBS, 25 M 3-isobutyl-1-methylxanthine, 100 nM dexamethasone, 1 M RGZ, 100 nM insulin, 0.2 nM and triiodothyronine for 3 d and thereafter maintained in L15 medium supplemented with 5% FBS, 100 nM insulin, 0.2 nM and triiodothyronine.…”

Section: -Nbdg Uptake Assaysmentioning

confidence: 99%

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

et al. 2012

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Isoorientin (ISO) is a plant C-glycosylflavonoid with purported antidiabetic effects but unexplored mechanisms of action. To gain insight into its antidiabetic mechanisms, we assayed nontoxic ISO concentrations on the 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2-deoxy-D-glucose (2-NBDG) uptake by murine 3T3-F442A and human sc adipocytes. In insulin-sensitive adipocytes, ISO stimulated the 2-NBDG uptake by 210% (murine) and 67% (human), compared with insulin treatment. Notably, ISO also induced 2-NBDG uptake in murine (139%) and human (60%) adipocytes made resistant to insulin by treatment with TNF-␣, compared with the incorporation induced in these cells by rosiglitazone. ISO induction of glucose uptake in adipocytes was abolished by inhibitors of the insulin signaling pathway. These inhibitors also blocked the proper phosphorylation of insulin signaling pathway components induced by ISO in both insulin-sensitive and insulinresistant adipocytes. Additionally, ISO stimulated the transcription of genes encoding components of insulin signaling pathway in murine insulin-sensitive and insulin-resistant adipocytes. In summary, we show here that ISO exerts its antidiabetic effects by activating the insulin signaling pathway in adipocytes, reverts the insulin resistance caused in these cells by TNF-␣ by stimulating the proper phosphorylation of proteins in this signaling pathway, and induces the expression of genes encoding these proteins. (Endocrinology 153: 5222-5230, 2012)

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“…was the most potent and it produced the highest reduction in the plasma glucose level that was even lower than the fasting level after 90 min, an effect that suggests an additive mechanism of action. This assumption could be true since this plant contains CGA which hits several targets in the diabetes metabolic pathways, besides its acarboselike effect [75,76] .…”

Section: Inhibition Of Glucose Absorptionmentioning

confidence: 99%

Phytotherapy in diabetes: Review on potential mechanistic perspectives

El‐Abhar

Schaalan

2014

WJD

106

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Diabetes mellitus (DM) is a widely spread epidemic disease that results from the absence of insulin, decreased secretion and/or impaired function. Since DM is a multifactorial disease, the available pharmaceuticals, despite their sensible treatment, target mostly one pathway to control hyperglycemia and encounter several side effects. Therefore, new therapeutic paradigms aim to hit several pathways using only one agent. Traditionally, antidiabetic plants and/or their active constituents may fulfill this need. More than 200 species of plants possess antidiabetic properties which were evaluated mostly by screening tests without digging far for the exact mode of action. Searching among the different literature resources and various database and in view of the above aspects, the present article provides a comprehensive review on the available antidiabetic plants that have been approved by pharmacological and clinical evaluations, and which their mechanism(s) of action is assured. These plants are categorized according to their proved mode of action and are classified into those that act by inhibiting glucose absorption from intestine, increasing insulin secretion from the pancreas, inhibiting glucose production from hepatocytes, or enhancing glucose uptake by adipose and muscle tissues. The current review also highlights those that mimic in their action the new peptide analogs, such as exenatide, liraglutide and dipeptidylpeptidase-4 inhibitors that increase glucagon-like peptide-1 serum concentration and slow down the gastric emptying.© 2014 Baishideng Publishing Group Co., Limited. All rights reserved.Key words: Hypoglycaemic nutraceuticals; Antidiabetic phyto-constituents; Medicinal plants; Phytotherapy; Diabetes mellitus Core tip: Diabetes is a serious metabolic disorder that is currently treated by different types of synthetic oral hypoglycemic agents, in addition to insulin. However, due to the unwanted side effects, the efficacies of these compounds are debatable and there is a demand for new compounds for the treatment of diabetes. Therefore, attention has been directed towards nutraceuticals originating from plants that are rich in antidiabetic phyto-constituents. Although the evidenced-based therapeutic usage of many plants is scarce, the plants cited in this review are those reputed traditionally for their antidiabetic effect and that were verified either experimentally or clinically.El-Abhar HS, Schaalan MF. Phytotherapy in diabetes: Review on potential mechanistic perspectives. World J Diabetes 2014; 5(2): 176-197 Available from:

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Cecropia obtusifolia Bertol and its active compound, chlorogenic acid, stimulate 2-NBDglucose uptake in both insulin-sensitive and insulin-resistant 3T3 adipocytes

Cited by 93 publications

References 17 publications

Bioassay-guided Isolation of the Antidiabetic Active Principle from Salvia miltiorrhiza and its Stimulatory Effects on Glucose Uptake Using 3T3-L1 Adipocytes

Bioassay-guided Isolation of the Antidiabetic Active Principle from Salvia miltiorrhiza and its Stimulatory Effects on Glucose Uptake Using 3T3-L1 Adipocytes

Isoorientin Reverts TNF-α-Induced Insulin Resistance in Adipocytes Activating the Insulin Signaling Pathway

Phytotherapy in diabetes: Review on potential mechanistic perspectives

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