Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing

Abstract

Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim of this study was to investigate the cecal microbiota of Tibetan Chickens from five typical high‐altitude regions of China. Lohmann egg‐laying hens (LMs) and Daheng broiler chickens (DHs) were chosen as controls. The cecal bacterial populations of Tibetan Chickens were surveyed by high‐throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3‐V4 (16S rRNAV3‐V4) combined with community‐fingerprinting analysis of the 16S rRNA gene based on polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE). The results revealed that the majority of cecal microbiota differed between the Tibetan Chicken and LM/DH. The microbial communities in the cecum were composed of 16 phyla, 28 classes, 36 orders, 57 families, 101 genera, and 189 species. Represented phyla were Bacteroidetes (>47%), Firmicutes (>18.8%), Spirochaetae (>0.3%), and Proteobacteria (>0.4%). Bacteroides and the RC9 gut group were the two most abundant genera. There were relatively more Christensenellaceae, Subdoligranulum, Spirochaeta, and Treponema in Tibetan Chickens, whereas there were more Phascolarctobacterium, Faecalibacterium, Megamonas, and Desulfovibrio in LMs and DHs. The cecal microbiota of Tibetan Chicken have slightly diverged due to exposure to different geographic environments. Differences in the intestinal bacterial communities of Tibetan Chicken and LM/DH were noted.

Effects of husbandry systems and Chinese indigenous chicken strain on cecum microbial diversity

“…The core microbiota of the 4 groups were counted at the phylum level (Table 4) and genus level (Table 5). At the phylum level, core members are microbes belonging to Bacteroidetes (49%-60%), Firmicutes (21%-32%) and Proteobacteria (2%~4%), which are similar to the results reported in broilers [20], Dagu chickens [21]and Tibetan Chickens [22]. It…”

Characterization and comparison of the bacterial microbiota in different gastrointestinal tract compartments of Mongolian horses

“…Compared with traditional isolation methods, the next‐generation sequencing appears more efficient to analyzing microbiome structures, especially for the species that are hard to cultivate in vitro (Zhang et al, 2016). Therefore, this technique had been used extensively to characterize the intestinal microbiota of several species (Kim, Gu, Lee, Joh, & Kim, 2012; Orpin, 1981; Wu et al, 2016; Yang et al, 2017; Zhang et al, 2016; Zhou et al, 2016). Present studies on the gut microbiota were focused on fecal samples, which only represent the microbial structures of the right dorsal colon but not the entire gut microbiota.…”

Age-Related Variations in Intestinal Microflora of Free-Range and Caged Hens

“…While recent research used PCR-DGGE analysis revealed that the microbial communities in the cecum of Tibetan Chickens (a local chicken in China) were composed of 16 phyla, moreover Bacteroidetes were represented in cecum at more than 47% (Zhou et al, 2016). These varied microbial community distinction in GI might are caused by different features of each section of GI (Han et al, 2016).…”