CE with MS detection: A rapidly developing hyphenated technique

Klampfl, Christian W.

doi:10.1002/elps.200900088

Cited by 87 publications

(73 citation statements)

References 119 publications

(139 reference statements)

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“…Nowadays, the hyphenation of effective separation techniques with an MS device plays an important role in the structural analysis of complex analyte mixtures [22]. HPLC-Chip/TOF-MS has been recently introduced as a potential analytical tool for the analysis of HMOs in breast milk [8,23].…”

Section: Introductionmentioning

confidence: 99%

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CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies

et al. 2010

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Mixtures of the complex human milk oligosaccharides (HMOs) are difficult to analyze and gastrointestinal bioconversion products of HMOs may complicate analysis even more. Their analysis, therefore, requires the combination of a sensitive and high-resolution separation technique with a mass identification tool. This study introduces for the first time the hyphenation of CE with an electrospray mass spectrometer, capable to perform multiple MS analysis (ESI-MS(n)) for the separation and characterization of HMOs in breast milk and feces of breast-fed babies. LIF was used for on- and off-line detections. From the overall 47 peaks detected in off-line CE-LIF electropherograms, 21 peaks could be unambiguously and 11 peaks could be tentatively assigned. The detailed structural characterization of a novel lacto-N-neo-tetraose isomer and a novel lacto-N-fucopentaose isomer was established in baby feces and pointed to gastrointestinal hydrolysis of higher-Mw HMOs. CE-LIF-ESI-MS(n) presents, therefore, a useful tool which contributes to an advanced understanding on the fate of individual HMOs during their gastrointestinal passage.

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Section: Introductionmentioning

confidence: 99%

“…They were introduced to a broad range of research fields (e.g. biomedical analysis) and are outstanding in view of separation efficiency and selectivity [22]. Regarding the Abbreviations: HMO, human milk oligosaccharides; APTS, 9-aminopyrene-…”

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confidence: 99%

CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies

et al. 2010

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“…CZE separates analytes by their charge-to-size ratios in buffers under a high electrical field and is often used as the final dimension prior to MS analysis, while the separation feature of CIEF is based on isoelectric point, and this technique is more suitable to be used as the first dimension separation. Detailed description of different CE-MS interfaces, sample preconcentration and capillary coating to minimize analyte adsorption could be found in several reviews (Ahmed, 2009a;Fonslow and Yates, 2009;Haselberg et al, 2007Haselberg et al, , 2011Huck et al, 2006;Klampfl, 2009;Simpson and Smith, 2005). CE technique is complementary to conventional LC in that it is suitable for the analysis of polar and chargeable compounds.…”

Section: Fractionation and Separationmentioning

confidence: 99%

Mass spectrometry-based proteomics and peptidomics for systems biology and biomarker discovery

Cunningham

2012

Front. Biol.

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The scientific community has shown great interest in the field of mass spectrometry-based proteomics and peptidomics for its applications in biology. Proteomics technologies have evolved to produce large datasets of proteins or peptides involved in various biological and disease progression processes producing testable hypothesis for complex biological questions. This review provides an introduction and insight to relevant topics in proteomics and peptidomics including biological material selection, sample preparation, separation techniques, peptide fragmentation, post-translation modifications, quantification, bioinformatics, and biomarker discovery and validation. In addition, current literature and remaining challenges and emerging technologies for proteomics and peptidomics are presented.

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“…MS, LIF) allow a wide range of applications [1][2][3][4][5]. Capillary electrophoresis coupled with mass spectrometry (CE-MS) was proved to be a powerful analytical tool for characterization, identification and quantification of chiral and/or achiral analytes from the small molecules to macro biomolecules present in the complex biological matrices [6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Some possibilities of an analysis of complex samples by a mass spectrometry with a sample pretreatment by an offline coupled preparative capillary isotachophoresis

et al. 2011

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This work deals with an analysis of biologically important compounds in complex matrices using preparative isotachophoresis (pITP) in column coupling configuration as a sample pretreatment technique followed by a direct infusion mass spectrometry with nano-electrospray ionization (DI-nESI-MS). Busereline was chosen as a model analyte, and urine was chosen as an example of complex matrix. In pITP experiments, sodium cation (10 mmol/L concentration) was used as a leading ion and β-alanine as terminating ion (20 mmol/L concentration). The fractions, obtained by pITP pre-separation with the assistance of the mixture of discrete spacers, were finally analyzed by DI-nESI-MS. It was shown that pITP performed before DI-nESI-MS analysis can significantly simplify complex matrix, and, due to its concentration power, pITP can consequently decrease the concentration limit of detection. The concentration of buserelin in the urine samples analyzed by pITP-DI-nESI-MS was 10 μg/L (reflecting at a 8.10⁻⁹ mol/L concentration) in our work but from the ion intensities obtained in MS as well as MS/MS analyses, it is clear that this concentration level could be several orders of magnitude lower for reliable detection and identification of buserelin in urine analyzed using pITP with DI-nESI-MS detection.

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CE with MS detection: A rapidly developing hyphenated technique

Cited by 87 publications

References 119 publications

CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies

CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies

Mass spectrometry-based proteomics and peptidomics for systems biology and biomarker discovery

Some possibilities of an analysis of complex samples by a mass spectrometry with a sample pretreatment by an offline coupled preparative capillary isotachophoresis

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CE with MS detection: A rapidly developing hyphenated technique

Cited by 87 publications

References 119 publications

CE‐LIF‐MSn profiling of oligosaccharides in human milk and feces of breast‐fed babies

CE‐LIF‐MSn profiling of oligosaccharides in human milk and feces of breast‐fed babies

Mass spectrometry-based proteomics and peptidomics for systems biology and biomarker discovery

Some possibilities of an analysis of complex samples by a mass spectrometry with a sample pretreatment by an offline coupled preparative capillary isotachophoresis

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CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies

CE‐LIF‐MSⁿ profiling of oligosaccharides in human milk and feces of breast‐fed babies