CE‐MS for metabolomics: Developments and applications in the period 2012–2014

Ramautar, Rawi; Somsen, Govert W.; Jong, Gerhardus J. de

doi:10.1002/elps.201400388

Cited by 120 publications

(79 citation statements)

References 63 publications

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“…A CE-MS vem sendo usada como técnica complementar a LC-MS e GC-MS, [38][39][40] pois promove a separação de compostos iônicos polares. Possui alta resolução e curtos tempos de análise.…”

Section: Figura 1 Esquema Resumido Do Fluxograma De Trabalho Envolviunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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Section: Figura 1 Esquema Resumido Do Fluxograma De Trabalho Envolviunclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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“…In LC-MS, the plant extract is usually re-dissolved in a solvent with the same composition of the initial conditions of the LC mobile phase. CE-MS, in turn, includes very low organic solvent consumption without the need for extensive sample pretreatment [45,46].…”

Section: (Iii) Pre-analytical Requirementsmentioning

confidence: 99%

“…Briefly, metabolites are first separated according to their charge-to-size ratio and then selectively detected and quantified in the mass spectrometer by their specific mass-tocharge ratio (m/z). The major advantages associated with CE-MS are the non-extensive sample pretreatment procedures that require very low organic solvent consumption, and the efficient and fast separations with low LOD [45,46,[109][110][111].…”

Section: (B) Ce-msmentioning

confidence: 99%

Mass spectrometry as a quantitative tool in plant metabolomics

Jorge

Mata

António

2016

Phil. Trans. R. Soc. A.

110

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Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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“…Today CE is increasingly used for several reasons: its high efficacy, versatility and feasibility of incorporating a large number of selectors in the background electrolyte that greatly facilitates method development, rapidity, cheapness (a low consumption of reagents), and minimum environmental impact with respect to HPLC or GC techniques. The high peak efficiency that can be obtained in CE (between 100,000--200,000 plates in a typical capillary) is one of the major advantages [32].…”

Section: Technological Approachesmentioning

confidence: 99%