CE-MS for Clinical Proteomics and Metabolomics: Strategies and Applications

Ramautar, Rawi; Britz‐McKibbin, Philip

doi:10.1002/9783527693801.ch11

Cited by 2 publications

(5 citation statements)

References 94 publications

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“…Importance of MS as analytical and structure elucidation method has significantly increased in the last years due to its crucial role in the previous omics technologies [44,199,202]. Hence, the relevance of MS hyphenation with CE separations of peptides and proteins is growing as well.…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…Being both universal and selective and providing high‐sensitivity and structurally rich information on the analyzed compounds, MS represents the most powerful detection mode for CE, LC, and other separation methods. The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE–MS in general [195–198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8, 16, 44, 199–201]. Importance of MS as analytical and structure elucidation method has significantly increased in the last years due to its crucial role in the previous omics technologies [44, 199, 202].…”

Section: Detectionmentioning

confidence: 99%

“…The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE–MS in general [195–198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8, 16, 44, 199–201]. Importance of MS as analytical and structure elucidation method has significantly increased in the last years due to its crucial role in the previous omics technologies [44, 199, 202]. Hence, the relevance of MS hyphenation with CE separations of peptides and proteins is growing as well.…”

Section: Detectionmentioning

confidence: 99%

“…The recent developments in the area of MS detection in CE methods have been described in several reviews dealing with CE-MS in general [195][196][197][198] and with its application to peptide, protein, peptidomic, proteomic, and metabolomic analyses especially [8,16,44,[199][200][201].…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…Nowadays, CE methods are appreciated as recognized counterparts and/or complements of the (U)HPLC (highperformance liquid chromatography) and nano-LC methods [36][37][38] that are mostly used for both analytical and preparative peptide separations. In addition to the last one of the previous reviews [35], the recent developments of CE and CEC separations of peptides were reported in some other reviews [39][40][41][42], books [43,44], and book chap-ters [22,45,46] dealing with particular aspects of CE and MCE of peptides, proteins, amino acids (AAs), and other biomolecules.…”

mentioning

confidence: 99%

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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This review article brings a comprehensive survey of developments and applications of high‐performance capillary and microchip electromigration methods (zone electrophoresis in a free solution or in sieving media, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, micropreparation, and physicochemical characterization of peptides in the period from 2021 up to ca. the middle of 2023. Progress in the study of electromigration properties of peptides and various aspects of their analysis, such as sample preparation, adsorption suppression, electroosmotic flow regulation, and detection, are presented. New developments in the particular capillary electromigration methods are demonstrated, and several types of their applications are reported. They cover qualitative and quantitative analysis of synthetic or isolated peptides and determination of peptides in complex biomatrices, peptide profiling of biofluids and tissues, and monitoring of chemical and enzymatic reactions and physicochemical changes of peptides. They include also amino acid and sequence analysis of peptides, peptide mapping of proteins, separation of stereoisomers of peptides, and their chiral analyses. In addition, micropreparative separations and physicochemical characterization of peptides and their interactions with other (bio)molecules by the above CE methods are described.

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Section: Mass Spectrometrymentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

Section: Detectionmentioning

confidence: 99%

Section: Mass Spectrometrymentioning

confidence: 99%

mentioning

confidence: 99%

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Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Kašička

2023

Electrophoresis

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CE–MS for metabolomics: Developments and applications in the period 2014–2016

2016

Self Cite

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CE–MS can be considered a useful analytical technique for the global profiling of (highly) polar and charged metabolites in various samples. Over the past few years, significant advancements have been made in CE–MS approaches for metabolomics studies. In this paper, which is a follow‐up of a previous review paper covering the years 2012–2014 (Electrophoresis 2015, 36, 212–224), recent CE–MS strategies developed for metabolomics covering the literature from July 2014 to June 2016 are outlined. Attention will be paid to new CE–MS approaches for the profiling of anionic metabolites and the potential of SPE coupled to CE–MS is also demonstrated. Representative examples illustrate the applicability of CE–MS in the fields of biomedical, clinical, microbial, plant, and food metabolomics. A complete overview of recent CE–MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, general conclusions and perspectives are given.

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CE-MS for Clinical Proteomics and Metabolomics: Strategies and Applications

Cited by 2 publications

References 94 publications

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

Recent developments in capillary and microchip electroseparations of peptides (2021–mid‐2023)

CE–MS for metabolomics: Developments and applications in the period 2014–2016

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