CDX2 does not suppress tumorigenicity in the human gastric cancer cell line MKN45

Dang, Long H.; Chen, F; Knock, S A; Huang, Emina H.; Jin, Feng; Appelman, Henry D.; Dang, Duyen T.

doi:10.1038/sj.onc.1209243

Cited by 16 publications

(17 citation statements)

References 94 publications

(112 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cdx2 may have a similar function in gastric cancer. However, Dang et al reported that Cdx2 does not suppress tumorigenicity in the human gastric cancer cell line MKN45 (26). Further investigations are necessary to fully elucidate the function and regulatory mechanism of Cdx2 expression, regardless of histological type or differentiation grade.…”

Section: Discussionmentioning

confidence: 99%

Homeoprotein Cdx2 and nuclear PTEN expression profiles are related to gastric cancer prognosis

Bai¹,

Ye²,

Chen

et al. 2007

APMIS

View full text Add to dashboard Cite

The aim of the study was to analyze the expression of Cdx2 and nuclear PTEN in relation to clinicopathological features of gastric cancer tissue biopsies in order to determine the value of a combined analysis of Cdx2 and nuclear PTEN expression in distinguishing histological types and prognosis of gastric cancers. The expression of Cdx2 and nuclear PTEN was studied using immunohistochemistry of paraffin-embedded tumor specimens from 99 patients who underwent radical D2 gastrectomy between 1999 and 2001. Cdx2 and nuclear PTEN expression were detected in 39.6% (36 of 91) and 70.3% (64 of 91) of gastric cancer cases, respectively. There was a negative correlation between Cdx2 expression and Lauren classification (p=0.032), and between nuclear PTEN expression and lymph node metastasis (p=0.049). Patients with Cdx2-positive, or nuclear PTEN-positive expression had higher survival rates than those with Cdx2-negative or nuclear PTEN-negative expression (p<0.001 and p=0.003, respectively). Co-expression of Cdx2 and nuclear PTEN showed significantly lower levels in diffuse- or mixed-type cancers than in intestinal-type cancers (p=0.005). Multivariate analysis revealed that Cdx2 expression was an independent prognostic indicator of gastric cancer (p=0.014). These data suggest that combined analysis of Cdx2 and nuclear PTEN expression can have significant value in distinguishing histological types of gastric cancer and assessing prognosis in patients with gastric cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Homeoprotein Cdx2 and nuclear PTEN expression profiles are related to gastric cancer prognosis

Bai¹,

Ye²,

Chen

et al. 2007

APMIS

View full text Add to dashboard Cite

show abstract

“…This demonstrated that Cdx2 may act as a tumor suppressor gene in the GC BGC823 cell line. However, it has been reported that Cdx2 does not suppress tumorigenicity in the human GC MKN45 cell line (25). Further investigations will be required to clarify the mechanisms that are involved in the regulation of Cdx2 expression.…”

Section: Discussionmentioning

confidence: 99%

PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells

Bai

Shen

et al. 2013

International Journal of Oncology

View full text Add to dashboard Cite

Abstract. The prognosis of gastric cancer (GC) is associated with Cdx2 and nuclear PTEN coexpression. This study aimed to determine the expression patterns of Cdx2 and PTEN in various GC tissues and cell lines to identify their relationship in GC. Immunohistochemistry was undertaken to assess the expression patterns of Cdx2 and PTEN in paraffin-embedded specimens of 228 GC patients who had undergone radical D2 gastrostomy with long-term follow-up. Cell growth and tumorigenicity were analyzed in the BGC823 cells with exogenous Cdx2 and any changes in the associated signaling pathways were interpreted in exogenous cdx2 expression and cdx2 knockdown. Cdx2 was found in the nuclei of GC cells in 43.4% (99/228) of the paraffin-embedded biopsies. A higher expression of nuclear PTEN was observed in 36.4% (83/228). Coexpression of Cdx2 and nuclear PTEN was detected in GC tumors (59/228, 25.9%) which correlated with the prognosis of advanced GC patients (p<0.001). The expression levels of Cdx2 and PTEN were variable in the different GC cell lines. However, the trends were similar between PTEN and Cdx2 in GC tissues and cell lines. High expression of Cdx2 and PTEN significantly reduced tumorigenicity in BGC823 cells compared with the empty vector control. Exogenous expression of Cdx2 triggered the upregulation of PTEN expression and decreased PI3K and pAkt expression and vice versa. The coexpression levels of PTEN and Cdx2 in GC tumors correlated with prognosis in GC patients. Cdx2 may play a role in the upregulation of PTEN by triggering PI3K/Akt inactivation in GC cells.

show abstract

“…CDX2 appears to contribute to oncogenic potential in a mouse model of leukemia and with this report, two colon cancer cell lines. However, we have observed that CDX2 does not suppress tumorigenicity in the human gastric cancer cell line MKN45 (Dang et al, 2006). Certainly, further investigations are warranted and ongoing.…”

Section: Cdx2 Has Tumorigenic Potential In Colon Cancer Cells Lh Dangmentioning

confidence: 95%

CDX2 has tumorigenic potential in the human colon cancer cell lines LOVO and SW48

et al. 2005

Self Cite

View full text Add to dashboard Cite

CDX2 does not suppress tumorigenicity in the human gastric cancer cell line MKN45

Cited by 16 publications

References 94 publications

Homeoprotein Cdx2 and nuclear PTEN expression profiles are related to gastric cancer prognosis

Homeoprotein Cdx2 and nuclear PTEN expression profiles are related to gastric cancer prognosis

PTEN expression and suppression of proliferation are associated with Cdx2 overexpression in gastric cancer cells

CDX2 has tumorigenic potential in the human colon cancer cell lines LOVO and SW48

Contact Info

Product

Resources

About