CdTe QD-based inhibition and reactivation assay of acetylcholinesterase for the detection of organophosphorus pesticides

Korram, Jyoti; Dewangan, Lakshita; Karbhal, Indrapal; Nagwanshi, Rekha; Vaishanav, Sandeep K.; Ghosh, Kallol K.; Satnami, Manmohan L.

doi:10.1039/d0ra03055d

Cited by 42 publications

(17 citation statements)

References 58 publications

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“…Quantum dots, which are semiconductor crystalline nanomaterials with unique optical properties due to quantum confinement effects, are an example of nanocomposite probes that have enhanced fluorescent detection for pesticide residue screening [ 66 ]. This was recently demonstrated for the detection of four OP pesticides, namely, paraoxon, dichlorvos, malathion, and triazophos, using CdTe quantum dots as the fluorescent probe coupled to an AChE-choline oxidase enzyme system [ 69 ]. In this case, when AChE was active (resulting in choline production), H 2 O 2 was produced by choline oxidase, which in turn “turned off” the FL of the CdTe quantum dots.…”

Section: Pesticide Residue Optical Screening In Food Matricesmentioning

confidence: 99%

Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends

Tsagkaris

Pulkrabová

Hajšlová

2021

Foods

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Pesticides have been extensively used in agriculture to protect crops and enhance their yields, indicating the need to monitor for their toxic residues in foodstuff. To achieve that, chromatographic methods coupled to mass spectrometry is the common analytical approach, combining low limits of detection, wide linear ranges, and high accuracy. However, these methods are also quite expensive, time-consuming, and require highly skilled personnel, indicating the need to seek for alternatives providing simple, low-cost, rapid, and on-site results. In this study, we critically review the available screening methods for pesticide residues on the basis of optical detection during the period 2016–2020. Optical biosensors are commonly miniaturized analytical platforms introducing the point-of-care (POC) era in the field. Various optical detection principles have been utilized, namely, colorimetry, fluorescence (FL), surface plasmon resonance (SPR), and surface enhanced Raman spectroscopy (SERS). Nanomaterials can significantly enhance optical detection performance and handheld platforms, for example, handheld SERS devices can revolutionize testing. The hyphenation of optical assays to smartphones is also underlined as it enables unprecedented features such as one-click results using smartphone apps or online result communication. All in all, despite being in an early stage facing several challenges, i.e., long sample preparation protocols or interphone variation results, such POC diagnostics pave a new road into the food safety field in which analysis cost will be reduced and a more intensive testing will be achieved.

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Section: Pesticide Residue Optical Screening In Food Matricesmentioning

confidence: 99%

Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends

Tsagkaris

Pulkrabová

Hajšlová

2021

Foods

View full text Add to dashboard Cite

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“…In this method, spectrofluorophotometer can generate the signal shift and be observed by the naked eye on-site [ 29 ]. Different types of recognition elements and materials have been used in the manufacturing of fluorescence sensors, including enzymes [ 30 ], semiconductor nanomaterials [ 31 ], metal nanomaterials [ 32 ], carbon materials [ 33 ] and noble metal [ 34 ], as it can enhance the sensitivity of the sensor.…”

Section: Optical Sensorsmentioning

confidence: 99%

Recent Advances on Detection of Insecticides Using Optical Sensors

Fauzi

Fen

Omar

et al. 2021

Sensors

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Insecticides are enormously important to industry requirements and market demands in agriculture. Despite their usefulness, these insecticides can pose a dangerous risk to the safety of food, environment and all living things through various mechanisms of action. Concern about the environmental impact of repeated use of insecticides has prompted many researchers to develop rapid, economical, uncomplicated and user-friendly analytical method for the detection of insecticides. In this regards, optical sensors are considered as favorable methods for insecticides analysis because of their special features including rapid detection time, low cost, easy to use and high selectivity and sensitivity. In this review, current progresses of incorporation between recognition elements and optical sensors for insecticide detection are discussed and evaluated well, by categorizing it based on insecticide chemical classes, including the range of detection and limit of detection. Additionally, this review aims to provide powerful insights to researchers for the future development of optical sensors in the detection of insecticides.

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“…Thus, there is an urgent need for an immediate, simple, and effective technique for real-time detection of residual pesticides. Biosensors have attracted more attention due to the high signal-to-noise ratio, excellent sensitivity, and easy operation. − They could employ different biological elements such as proteins, enzymes, DNA, cells, and even whole live organisms as recognition hosts to detect different pesticides as guests. − Among them, enzyme inhibition-based biosensors integrating with optical probes received tremendous attention owning to their typical advantages such as high sensitivity and selectivity, simple operation, fast response, and good stability. ,− However, the real-time detection of pesticide residues by such enzyme-based biosensors has been severely limited by substantial structure diversity of pesticides and their diverse action modes. Besides, the diffusion/dilution of the fluorescent signal for the enzyme-activated fluorescent probes impedes the in situ detection and differentiation, which has never been considered for monitoring pesticides in the past …”

Section: Introductionmentioning

confidence: 99%

Multienzyme-Targeted Fluorescent Probe as a Biosensing Platform for Broad Detection of Pesticide Residues

Guo

Liu

et al. 2021

Anal. Chem.

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Pesticide residues, significantly hampering the overall environmental and human health, have become an increasingly severe issue. Thus, developing rapid, cost-effective, and sensitive tools for monitoring the pesticide residues in food and water is extremely important. Compared to the conventional and chromatographic techniques, enzyme inhibition-based biosensors conjugated with the fluorogenic probes provide effective alternative methods for detecting pesticide residues due to the inherent advantages including high selectivity and sensitivity, simple operation, and capability of providing in situ and real-time information. However, the detection efficiency of a single enzyme-targeted biosensor in practical samples is strongly impeded by the structural diversity of pesticides and their distinct targets. In this work, we developed a strategy of multienzyme-targeted fluorescent probe design and accordingly obtained a novel fluorescent probe (named as 3CP) for detecting the presence of wide variety of pesticides. The designed probe 3CP, targeting cholinesterases, carboxylesterases, and chymotrypsin simultaneously, yielded intense fluorescence in the solid state upon the enzyme-catalyzed hydrolysis. It showed excellent sensitivity against organophosphorus and carbamate pesticides, and the detection limit for dichlorvos achieved 1.14 pg/L. Moreover, it allowed for the diffusion-resistant in situ visualization of pesticides in live cells and zebrafish and the sensitive measurement of organophosphorus pesticides in fresh vegetables, demonstrating the promising potential for tracking the pesticide residues in environment and biological systems.

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CdTe QD-based inhibition and reactivation assay of acetylcholinesterase for the detection of organophosphorus pesticides

Abstract: An enzyme immobilized glutathione (GSH)-capped CdTe quantum dot (QD)-based fluorescence assay has been developed for monitoring organophosphate pesticides.

Cited by 42 publications

References 58 publications

Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends

Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends

Recent Advances on Detection of Insecticides Using Optical Sensors

Multienzyme-Targeted Fluorescent Probe as a Biosensing Platform for Broad Detection of Pesticide Residues

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