CdSe/ZnS quantum dot–Cytochrome c bioconjugates for selective intracellular O2˙− sensing

Li, Dawei; Qin, Lixia; Li, Yang; Nia, Raheleh Partovi; Long, Yi‐Tao; Chen, Hong‐Yuan

doi:10.1039/c1cc13160e

Cited by 54 publications

(30 citation statements)

References 24 publications

(24 reference statements)

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“…29,30 Among them, QDs are one of the most prominent candidates due to their high fluorescence quantum yield (QY), broad excitation, and size-dependent emission tunability, as well as excellent photostability. 31,32 Currently, QDs have been promoted as ideal fluorescent labels for developing highly sensitive and multiplex immunoassays, [33][34][35][36] yet QDs labels have not gained acceptance into standard clinical practice and their potential is largely limited due to either physical or chemical instability in biological solutions and media.…”

mentioning

confidence: 99%

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Zhang¹,

Bao²,

Draz³

et al. 2015

IJN

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Convenient and rapid immunofiltration assays (IFAs) enable on-site “yes” or “no” determination of disease markers. However, traditional IFAs are commonly qualitative or semi-quantitative and are very limited for the efficient testing of samples in field diagnostics. Here, we overcome these limitations by developing a quantum dots (QDs)-based fluorescent IFA for the quantitative detection of C-reactive proteins (CRP). CRP, the well-known diagnostic marker for acute viral and bacterial infections, was used as a model analyte to demonstrate performance and sensitivity of our developed QDs-based IFA. QDs capped with both polyethylene glycol (PEG) and glutathione were used as fluorescent labels for our IFAs. The presence of the surface PEG layer, which reduced the non-specific protein interactions, in conjunction with the inherent optical properties of QDs, resulted in lower background signal, increased sensitivity, and ability to detect CRP down to 0.79 mg/L with only 5 µL serum sample. In addition, the developed assay is simple, fast and can quantitatively detect CRP with a detection limit up to 200 mg/L. Clinical test results of our QD-based IFA are well correlated with the traditional latex enhance immune-agglutination aggregation. The proposed QD-based fluorescent IFA is very promising, and potentially will be adopted for multiplexed immunoassay and in field point-of-care test.

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mentioning

confidence: 99%

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Zhang¹,

Bao²,

Draz³

et al. 2015

IJN

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“…For the neutrophils, the maximum current of ca. 16.0 nA corresponds to about 24.7 nM H 2 O 2 as calculated from the calibration curve in the inset of Fig. 5B.…”

Section: Detection Of H 2 O 2 Released From Living Cellsmentioning

confidence: 99%

“…Among them, electrochemical biosensors have received considerable attention because they can provide the direct, rapid, and real-time measurements with simple operation, low cost, high selectivity and sensitivity. It needs to be mentioned that electrochemical detection is also a powerful and attractive tool for monitoring other electroactive metabolites such as glucose [14], dopamine [15] and O 2 [16] secreted or taken up by living cells.…”

Section: Introductionmentioning

confidence: 99%

Detection of cellular H2O2 in living cells based on horseradish peroxidase at the interface of Au nanoparticles decorated graphene oxide

Wang

et al. 2015

Sensors and Actuators B: Chemical

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“…Thus, nanosystems that provide concentration-dependent signals may be more promising for this purpose; sensors based on the quenching of metallic nanoparticle fluorescence show such a response in vitro. For example, CdSe/ZnS quantum dots conjugated to oxidized cytochrome c; oxidation by O 2 -enhances fluorescence (113). Further along these lines, oxidation-responsive nanoparticles could also be employed to develop an ROS-detecting magnetic resonance imaging contrast system via release of Gd-based contrast agents from hydrophobic assemblies and resulting in de-quenching of contrast enhancement (188).…”

Section: Perspectivesmentioning

confidence: 99%

Exploiting Oxidative Microenvironments in the Body as Triggers for Drug Delivery Systems

Joshi-Barr

Lux

Mahmoud

et al. 2014

Antioxidants & Redox Signaling

118

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Significance: Reactive oxygen species and reactive nitrogen species (ROS/RNS) play an important role in cell signaling pathways. However, the increased production of these species may disrupt cellular homeostasis, giving rise to pathological conditions. Biomaterials that are responsive to ROS/RNS can be strategically used to specifically release therapeutics and diagnostic agents to regions undergoing oxidative stress. Recent Advances: Many nanocarriers intended to exploit redox micro-environments as triggers for drug release, summarized and compared in this review, have recently been developed. We describe these carriers' chemical structures, strategies for payload protection and oxidation-selective release, and ROS/RNS sensitivity as tested in initial studies. Critical Issues: ROS/RNS are unstable, so reliable measures of their concentrations in various conditions are scarce. Combined with the dearth of materials shown to respond to physiologically relevant levels of ROS/RNS, evaluations of their true sensitivity are difficult. Future Directions: Oxidation-responsive nanocarriers developed thus far show tremendous potential for applicability in vivo; however, the sensitivity of these chemistries needs to be fine tuned to enable responses to physiological levels of ROS and RNS. Antioxid. Redox Signal. 21, 730-754.

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CdSe/ZnS quantum dot–Cytochrome c bioconjugates for selective intracellular O2˙− sensing

Cited by 54 publications

References 24 publications

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Rapid and quantitative detection of C-reactive protein based on quantum dots and immunofiltration assay

Detection of cellular H2O2 in living cells based on horseradish peroxidase at the interface of Au nanoparticles decorated graphene oxide

Exploiting Oxidative Microenvironments in the Body as Triggers for Drug Delivery Systems

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