CDPKs: The critical decoders of calcium signal at various stages of malaria parasite development

Sharma, Manish; Choudhury, Himashree; Roy, Rajarshi; Michaels, Samantha A.; Ojo, Kayode K.; Bansal, Abhisheka

doi:10.1016/j.csbj.2021.08.054

Cited by 6 publications

(2 citation statements)

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“…Due of their absence from their mammalian hosts, they are regarded as potential druggable targets. In Plasmodium spp., CDPKs play essential roles at various critical physiological stages during parasite development, including merozoite invasion and asexual intraerythrocytic development in humans and sexual/pre-erythrocytic development in Anopheles mosquitoes [ 61 ]. Currently, fourteen CDPKs have been identified that contribute to several functions in the biological processes of T. gondii , such as gliding motility, cell invasion, and egress [ 62 ].…”

Section: Intraparasitic Egress Signalingmentioning

confidence: 99%

Egress Regulatory Factors: How Toxoplasma Exits from Infected Cells?

et al. 2023

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Toxoplasma gondii is an obligatory intracellular protozoan in the family Apicomplexa. It infects almost one-third of the world’s population and causes toxoplasmosis, a prevalent disease. The parasite’s egress from infected cells is a key step in the pathology caused by T. gondii. Moreover, T. gondii’s continuous infection relies heavily on its capacity to migrate from one cell to another. Many pathways are involved in T. gondii egress. Individual routes may be modified to respond to various environmental stimuli, and many paths can converge. Regardless of the stimuli, the relevance of Ca2+ as a second messenger in transducing these signals, and the convergence of various signaling pathways in the control of motility and, ultimately, egress, is well recognized. This review attempts to outline intra- and extra-parasitic regulators that mediate T. gondii egress, and provides insight into potential clinical interventions and research.

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Section: Intraparasitic Egress Signalingmentioning

confidence: 99%

Egress Regulatory Factors: How Toxoplasma Exits from Infected Cells?

et al. 2023

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“…PKAc1 suppresses egress in T. gondii but is instead required for invasion by P. falciparum 10,12,68−71 . The CDPK family of proteins are also required for P. falciparum and T. gondii egress and invasion, although the direct orthologs (de ned by reciprocal blast) do not always have the same function between species (Reviewed in 72,73 ). PLPs are involved in host cell permeabilization and egress in T. gondii, Plasmodium spp.…”

Section: Identi Cation Of Putative Egress Motility and Invasion Genes...mentioning

confidence: 99%

Babesia divergens egress from host cells is orchestrated by essential and druggable kinases and proteases

Elsworth

Keroack

Rezvani

et al. 2023

Preprint

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Apicomplexan egress from host cells is fundamental to the spread of infection and is poorly characterized in Babesia spp., parasites of veterinary importance and emerging zoonoses. Through the use of video microscopy, transcriptomics and chemical genetics, we have implicated signaling, proteases and gliding motility as key drivers of egress by Babesia divergens. We developed reverse genetics to perform a knockdown screen of putative mediators of egress, identifying kinases and proteases involved in distinct steps of egress (ASP3, PKG and CDPK4) and invasion (ASP2, ASP3 and PKG). Inhibition of egress leads to continued intracellular replication, indicating exit from the replication cycle is uncoupled from egress. Chemical genetics validated PKG, ASP2 and ASP3 as druggable targets in Babesia spp. All taken together, egress in B. divergens more closely resembles T. gondii than the more evolutionarily-related Plasmodium spp. We have established a molecular framework for biological and translational studies of B. divergens egress.

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