“…It combines simplicity, reproducibility, reasonable throughput ratio, and easy isolation of bands for sequencing. The cDNA-SRAP technique, with cDNA as a template, has been proven to be suitable for analysis of differential gene expression in several plants, including Brassica oleracea (Li et al, 2003;Deng et al, 2007), Spartina angelica (Lu and Wu, 2006), B. napus (Ma et al, 2008) and Erianthus arundinaceum (Que et al, 2012b). Furthermore, the cDNA-SRAP method, together with agarose gel electrophoresis, can overcome the shortcomings of the isotope labeling and silver staining methods by providing a shorter, safer, and less expensive method that requires no special equipment and can be conducted in a typical laboratory (Que et al, 2012b).…”