cDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome

Lü, Fangli; Jiang, Hongying; Ding, Jinhui; Mu, Jianbing; Valenzuela, Jesús G.; Ribeiro, José M. C.; Su, Xin‐zhuan

doi:10.1186/1471-2164-8-255

Cited by 54 publications

(54 citation statements)

References 45 publications

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“…In this study, the ToxoDB data sets were used to search MS/MS peptides against the hypothetical T. gondii proteome (mainly based on ME49 strain), which comprised all computationally predicted and experimental sequences available [22][23][24]. The database contains protein data from approximately 8000 genes of strain ME49 (http://toxodb.org/ common/downloads/release-5.2/Tgondii/).…”

Section: Discussionmentioning

confidence: 99%

Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

et al. 2010

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Toxoplasma gondii infection in pregnant women may result in abortion and foetal abnormalities, and may be life-threatening in immunocompromised hosts. To identify the potential infection markers of this disease, 2-DE and Western blot methods were employed to study the parasite circulating antigens and host-specific proteins in the sera of T. gondii-infected individuals. The comparisons were made between serum protein profiles of infected (n=31) and normal (n=10) subjects. Antigenic proteins were identified by immunoblotting using pooled sera and monoclonal anti-human IgM-HRP. Selected protein spots were characterised using mass spectrometry. Prominent differences were observed when serum samples of T. gondii-infected individuals and normal controls were compared. A significant up-regulation of host-specific proteins, α(2)-HS glycoprotein and α(1)-B glycoprotein, was also observed in the silver-stained gels of both active and chronic infections. However, only α(2)-HS glycoprotein and α(1)-B glycoprotein in the active infection showed immunoreactivity in Western blots. In addition, three spots of T. gondii proteins were detected, namely (i) hypothetical protein chrXII: 3984434-3 TGME 49, (ii) dual specificity protein phosphatase, catalytic domain TGME 49 and (iii) NADPH-cytochrome p450 reductase TGME 49. Thus, 2-DE approach followed by Western blotting has enabled the identification of five potential infection markers for the diagnosis of toxoplasmosis: three are parasite-specific proteins and two are host-specific proteins.

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Section: Discussionmentioning

confidence: 99%

Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

et al. 2010

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“…As the transcriptome of P. falciparum continues to be studied, new and/or alternative splice junctions are being discovered and the parasite may need some of these alternatively spliced gene products for either transcriptional or translational regulation. [33][34][35][36] For example, Sorber and colleagues 36 used an in-house developed splice site detection algorithm (HMMSplicer) and found a total of 982 new splice junctions absent from current Plasmodium models. They also found 310 alternative splicing events that occurred in 254 genes during the erythrocytic stages.…”

Section: Resultsmentioning

confidence: 99%

PFE0565w, a Plasmodium falciparum Protein Expressed in Salivary Gland Sporozoites

Schlarman¹,

Roberts²,

Kariuki³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Because malaria is still a significant problem worldwide, additional control methods need to be developed. The Plasmodium sporozoite is a good target for control measures because it displays dual infectivity for both mosquito and vertebrate host tissues. The Plasmodium falciparum gene, PFE0565w, was chosen as a candidate for study based on data from PlasmoDB, the Plasmodium database, indicating that it is expressed both at the transcriptional and protein levels in sporozoites, likely encodes a putative surface protein, and may have a potential role in the invasion of host tissues. Additional sequence analysis shows that the PFE0565w protein has orthologs in other Plasmodium species, but none outside of the genus Plasmodium. PFE0565w expresses transcript during both the sporozoite and erythrocytic stages of the parasite life cycle, where an alternative transcript was discovered during the erythrocytic stages. Data show that transcript is not present during axenic exoerythrocytic stages. Despite transcript being present in several life cycle stages, the PFE0565w protein is present only during the salivary gland sporozoite stage. Because the PFE0565w protein is present in salivary gland sporozoites, it could be a novel candidate for a pre-erythrocytic stage vaccine.

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“…In addition, several proteins are predicted to possess an N-terminal transit peptide but are missing an ER-type signal sequence, essential for entry into the secretory system ( Table 1). Prediction of intron/exon boundaries is notoriously challenging in the P. falciparum system (33), and a plausible explanation for the lack of a signal peptide suggests that the missing N-terminal signal can be found on a 5Ј exon which has not been included in the gene model. Indeed, based on cDNA sequences, we have previously reannotated the gene model for PFI0810c (encoding PfsUfd1) to include a signal peptide encoded on a previously "missed" 5Ј exon (49).…”

Section: Identification Of Aperad Proteins In Apicomplexamentioning

confidence: 99%

An Unusual ERAD-Like Complex Is Targeted to the Apicoplast ofPlasmodium falciparum

et al. 2009

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Many apicomplexan parasites, including Plasmodium falciparum, harbor a so-called apicoplast, a complex plastid of red algal origin which was gained by a secondary endosymbiotic event. The exact molecular mechanisms directing the transport of nuclear-encoded proteins to the apicoplast of P. falciparum are not well understood. Recently, in silico analyses revealed a second copy of proteins homologous to components of the endoplasmic reticulum (ER)-associated protein degradation (ERAD) system in organisms with secondary plastids, including the malaria parasite P. falciparum. These proteins are predicted to be endowed with an apicoplast targeting signal and are suggested to play a role in the transport of nuclear-encoded proteins to the apicoplast. Here, we have studied components of this ERAD-derived putative preprotein translocon complex in malaria parasites. Using transfection technology coupled with fluorescence imaging techniques we can demonstrate that the N terminus of several ERAD-derived components targets green fluorescent protein to the apicoplast. Furthermore, we confirm that full-length PfsDer1-1 and PfsUba1 (homologues of yeast ERAD components) localize to the apicoplast, where PfsDer1-1 tightly associates with membranes. Conversely, PfhDer1-1 (a host-specific copy of the Der1-1 protein) localizes to the ER. Our data suggest that ERAD components have been "rewired" to provide a conduit for protein transport to the apicoplast. Our results are discussed in relation to the nature of the apicoplast protein transport machinery.

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cDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome

Cited by 54 publications

References 45 publications

Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

Detection of immunogenic parasite and host‐specific proteins in the sera of active and chronic individuals infected with Toxoplasma gondii

PFE0565w, a Plasmodium falciparum Protein Expressed in Salivary Gland Sporozoites

An Unusual ERAD-Like Complex Is Targeted to the Apicoplast ofPlasmodium falciparum

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