cDNA sequences for pea disease resistance response genes

Fristensky, Brian; Horovitz, Daniel; Hadwiger, Lee A.

doi:10.1007/bf00017470

Cited by 117 publications

(60 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pisi (a fungus) and Pseudomonas syringae pv. pisi (a bacterium), respectively (Fristensky et al, 1988). However, it is unknown at present whether the I 49 gene product is directly active in plant defence against Fusarium or Pseudomonas (Lamb et al, 1989 The mRNA for BetvI was not only found in pollen and leaves, but also in male and female influorescences and phytohormone-dependent plant tissue culture (K.Pettenburger et al, in preparation).…”

Section: Discussionmentioning

confidence: 99%

The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene.

et al. 1989

View full text Add to dashboard Cite

Pollen of the white birch (Betula verrucosa) is one of the main causes of Type I allergic reactions (allergic rhinoconjunctivitis, allergic bronchial asthma) in Middle and Northern Europe, North America and the USSR. Type I allergies are a major threat to public health in these countries, since 10‐15% of the population suffer from these diseases. BetvI, an allergenic protein with an Mr of 17 kd is a constituent of the pollen of white birch and is responsible for IgE binding in more than 95% of birch pollen allergic patients. Here, we report the complete nucleotide sequence and deduced amino acid sequence of a cDNA clone coding for the major pollen allergen (BetvI) of white birch. It is similar to the N‐terminal peptide sequences of the allergens of hazel, alder and hornbeam (close relatives) but it has no significant sequence homology to any other known allergens. However, it shows 55% sequence identity with a pea disease resistance response gene, indicating that BetvI may be involved in pathogen resistance of pollen.

show abstract

Section: Discussionmentioning

confidence: 99%

The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene.

et al. 1989

View full text Add to dashboard Cite

show abstract

“…Analyzing the nucleotide sequences of the rCor a I isoforms, there is striking similarity with the conserved regions of other major tree pollen allergens of the order Fagales ( A h g I [2], Bet v I [3], Car b I [9]) as well as with pathogenesisrelated proteins from distantly related species like bean, pea, parsley and potato expressed in somatic tissues upon chemical or microbial stress [15][16][17]241. One might speculate that the Cor a I protein possesses a similar biological function in the protection of the pollen grains from microbial or fungal attack [25].…”

Section: Discussionmentioning

confidence: 99%

Four recombinant isoforms of Cor a I, the major allergen of hazel pollen, show different IgE‐binding properties

Breiteneder

Ferreira

Hoffmann‐Sommergruber

et al. 1993

European Journal of Biochemistry

201

130

View full text Add to dashboard Cite

Previous studies showed that pollens from trees of the order Fagales (e. g. birch, alder, hazel and hornbeam) all contain one major allergen. These proteins are cross-reactive between these tree species, and approximately 95 % of tree-pollen-allergic patients display IgE binding to these allergens. Using the reported N-terminal amino acid sequence of the hazel pollen allergen Cor a I, it was possible to amplify Cor-a-I cDNA by use of the polymerase chain reaction. Four clones with cDNA inserts were isolated. All four clones contained an open reading frame of 477 nucleotides (159 amino acids) but differed in length of their 3'-non-coding regions. Within the overlapping regions, the nucleotide sequence of the 3'-non-coding regions of the four clones were nearly identical. The open reading frames coded for different isoforms of the major hazel pollen allergen, Cor a I. The clones were designated Cor a Y5, 6, 11 and 16, respectively. Comparison of the deduced amino acid sequences of these Cor a 1 isoforms revealed identities of 96-99%. The sequence identities between the Cor a I isoforms and Bet v I, the major birch pollen allergen, were 71 -73 % (80.5-83% similarity). Comparing amino acid sequences of Cor a I isoforms with the published sequences of Aln g I, the major allergen from alder, and Car b I and isoforms, the major allergen from hornbeam, 75.5-76.7% identity (83.6-85% similarity) and 83.6-89.9% sequence identity (89.3-95% similarity), respectively, was found. The four Cor a I cDNAs were subcloned into plasmid pKK223-3 and expressed in Escherichia coli as non-fusion proteins; their capacity to bind serum IgE from tree-pollen-allergic patients was investigated. The four cloned isoforms showed an apparent molecular mass of 17 kDa in SDSPAGE, identical to the natural, pollen-derived Cor a 1. IgE antibodies from tree-pollen-allergic patients reacted with all four recombinant isoforms. However, we noted marked differences in the IgE-binding patterns of the distinct isoforms. Furthermore, Cor a Y l l was the only isoform recognized by the anti-(Bet v I) mAb, BIP 1. Our results demonstrate that Cor a I isoforms display different antigenic and allergenic properties, very likely due to few but significant changes in their amino acid sequences. These findings have implications for the development of reagents for diagnosis and immunotherapy of type I allergies.At least 15 % of the population of industrialized countries in the Northern hemisphere suffer from type I, i. e. IgE-mediated, allergies. In this respect, the pollens of early flowering trees of the order Fagales (in particular birch, alder, hazel and hornbeam) represent an important source of allergens. Numerous studies have been carried out to identify and characterize precisely the allergens of these pollens [ 1 -61.The major allergen from birch pollen, Bet v I, has been cloned, sequenced and expressed from a cDNA library [3]. Abbreviations. Aln g I, major allergen from alder pollen; Bet v I, major allergen from birch pollen; BIP 1, anti-(Bet v I) mAb; Car b...

show abstract

“…Comparison of the nucleotide sequence of the cloned allergen with the sequences in the EMBL databank known at that time revealed a sequence similarity of 70% to a pea disease resistance response gene (Fristensky et al, 1988). Since then, a growing new class of small intracellular defense-related proteins, different from all previously characterized pathogenesis-related proteins, has been described in several plant species.…”

Section: Introductionmentioning

confidence: 98%

The Potential ofBetv1Homologues, a Nuclear Multigene Family, as Phylogenetic Markers in Flowering Plants

Wen

Vanek-Krebitz

Hoffmann‐Sommergruber

et al. 1997

Molecular Phylogenetics and Evolution

View full text Add to dashboard Cite

cDNA sequences for pea disease resistance response genes

Cited by 117 publications

References 18 publications

The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene.

The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene.

Four recombinant isoforms of Cor a I, the major allergen of hazel pollen, show different IgE‐binding properties

The Potential ofBetv1Homologues, a Nuclear Multigene Family, as Phylogenetic Markers in Flowering Plants

Contact Info

Product

Resources

About