cDNA sequence, genomic organization and mapping of PDE6D, the human gene encoding the delta subunit of the cGMP phosphodiesterase of retinal rod cells to chromosome 2q36

Ershova, G.V.; Derré, Josette; Chételin, S.; Nancy, Vanessa; Berger, R; Jc, Kaplan; Münnich, Arnold; Gunzburg, Jean de

doi:10.1159/000134701

Cited by 3 publications

(1 citation statement)

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“…Intact COOH Terminus-We had previously isolated PDE␦ in yeast two-hybrid screens with several GTPases of the Ras superfamily as baits (20,28). As depicted in Table I Table I shows that it neither interacted with proteins carrying a COOH-terminal truncation, nor with the Ras mutant Val 12 3 Ser 186 where the Cys target of farnesylation has been replaced by a Ser.…”

Section: The Pde␦ Protein Interacts With Gtpases Containing Anmentioning

confidence: 99%

The δ Subunit of Retinal Rod cGMP Phosphodiesterase Regulates the Membrane Association of Ras and Rap GTPases

Nancy¹,

Callebaut²,

Marjou³

et al. 2002

Journal of Biological Chemistry

110

103

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Post-translational modifications of GTPases from the Ras superfamily enable them to associate with membrane compartments where they exert their biological activities. However, no protein acting like Rho and Rab dissociation inhibitor (GDI) that regulate the membrane association of Rho and Rab GTPases has been described for Ras and closely related proteins. We report here that the ␦ subunit of retinal rod phosphodiesterase (PDE␦) is able to interact with prenylated Ras and Rap proteins, and to solubilize them from membranes, independently of their nucleotide-bound (GDP or GTP) state. We show that PDE␦ exhibits striking structural similarities with RhoGDI, namely conservation of the Ig-like fold and presence of a series of hydrophobic residues which could act as in RhoGDI to sequester the prenyl group of its target proteins, thereby providing structural support for the biochemical activity of PDE␦. We observe that the overexpression of PDE␦ interferes with Ras trafficking and propose that it may play a role in the process that delivers prenylated proteins from endomembranes, once they have undergone proteolysis and carboxymethylation, to the structures that ensure trafficking to their respective resident compartments.

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Section: The Pde␦ Protein Interacts With Gtpases Containing Anmentioning

confidence: 99%

The δ Subunit of Retinal Rod cGMP Phosphodiesterase Regulates the Membrane Association of Ras and Rap GTPases

Nancy¹,

Callebaut²,

Marjou³

et al. 2002

Journal of Biological Chemistry

110

103

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Structure and Function of Rod Photoreceptors

Chen¹,

Geller²,

Flannery³

2006

Retina

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Analysis of PDE6D and PDE6G genes for generalised progressive retinal atrophy (gPRA) mutations in dogs

Dekomien

Epplen

2003

Genet Sel Evol

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The delta and gamma subunits of the cGMP-phosphodiesterase (PDE6D, PDE6G) genes were screened in order to identify mutations causing generalised progressive retinal atrophy (gPRA) in dogs. In the PDE6D gene, single nucleotide polymorphisms (SNP) were observed in exon 4, in introns 2 and 3 and in the 3' untranslated region (UTR) of different dog breeds. In the coding region of the PDE6G gene, exclusively healthy Labrador Retrievers showed an A-->G transition in exon 4 without amino acid exchange. SNP were also observed in introns 1 and 2 in different dog breeds. The different SNP were used as intragenic markers to investigate the involvement of both genes in gPRA. The informative substitutions allowed us to exclude mutations in the PDE6D and PDE6G genes as causing retinal degeneration in 15 of the 22 dog breeds with presumed autosomal recessively transmitted (ar) gPRA.

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cDNA sequence, genomic organization and mapping of PDE6D, the human gene encoding the delta subunit of the cGMP phosphodiesterase of retinal rod cells to chromosome 2q36

Cited by 3 publications

References 1 publication

The δ Subunit of Retinal Rod cGMP Phosphodiesterase Regulates the Membrane Association of Ras and Rap GTPases

The δ Subunit of Retinal Rod cGMP Phosphodiesterase Regulates the Membrane Association of Ras and Rap GTPases

Structure and Function of Rod Photoreceptors

Analysis of PDE6D and PDE6G genes for generalised progressive retinal atrophy (gPRA) mutations in dogs

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