cDNA for S-Adenosyl-L-Homocysteine Hydrolase from Catharanthus roseus

Schröder, G.; Waitz, Andreas; Hotze, Michael; Schröder, Joachim

doi:10.1104/pp.104.3.1099

Cited by 13 publications

(8 citation statements)

References 3 publications

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“…Similarly, it has recently been demonstrated that the phytoalexin response in legumes requires increased expression of NADP-malic enzyme (Schaaf et al 1995) and key enzymes of the oxidative pentose phosphate pathway (Fahrendorf et al 1995). In addition to alfalfa the induction of transcripts encoding enzymes of the activated methyl cycle by fungal infection or elicitor treatment has been reported in parsley plants and cell cultures (Kawalleck et al (1992) and cell cultures of Catharanthus roseus (Schroder et al 1994). Since these species produce methylated coumarins and alkaloids during their respective phytoalexin responses it is probable that, as is the case in alfalfa, the increased Fig.…”

Section: Discussionmentioning

confidence: 95%

“…It was therefore noteworthy that treatment of parsley leaves and cell cultures with a fungal elicitor induced the accumulation of transcripts encoding SAM synthetase and S-adenosyl-L-homocysteine (SAH) hydrolase (Kawalleck et al 1992). Similarly, SAH hydrolase expression is increased in cell cultures of Catharanthus roseus exposed to fungal elicitors (Schroder et al 1994). Since SAM synthetase and SAH hydrolase regulate SAM availability, their increased expression would be anticipated to coincide with increased transmethylation reactions in the plant cells.…”

Section: Introductionmentioning

confidence: 96%

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Methylation reactions and the phytoalexin response in alfalfa suspension cultures

Edwards

Daniell

Gregory

1997

Planta

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In order to determine why the activated methyl cycle is up-regulated in plants undergoing defence responses to fungal pathogens we have monitored the utilisation of methyl groups derived from methionine in cell-suspension cultures of alfalfa (Medicago sativa L.) treated for various times with fungal elicitor, by carrying out a parallel labelling study with [(35)S]methionine and [methyl-(3)H]methionine. The distribution of the two radiolabels among the medium, soluble cellular components and cell wall was then determined. In the absence of elicitor the utilisation of the two radiolabels was similar. However, in the presence of the elicitor the total incorporation of radioactivity from [methyl-(3)H]methionine into metabolites was far greater than from [(35)S]methionine, indicating that the methyl label had been utilised in methylation reactions. Elicitor treatment resulted in up to a sixfold increase in the use of (3)H-methyl groups in the methylation of hydrophobic metabolites. In the period 0-24 h after elicitor treatment, increased methylation was directed largely into the synthesis of the isoflavonoid phytoalexin medicarpin and related metabolites. Newly synthesized phytoalexins were exported into the medium, while a significant proportion of the medicarpin accumulating in the cell in the early stages of elicitation was derived from the hydrolysis of its respective conjugate. Elicitor treatment also modified the incorporation of (3)H-methyl groups into the cell wall. Between 0 and 24 h after elicitor treatment the methylation of pectin in the cell wall declined. After 24 h, pectin methylation recovered and was associated with an increase in the methylation of other wall-bound polysaccharide components. Since no other major metabolic sink for the increased methylation was determined we conclude that the increased activity of the activated methyl cycle during defence interactions in alfalfa is required to support phytoalexin synthesis and cell wall modifications.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 96%

Methylation reactions and the phytoalexin response in alfalfa suspension cultures

Edwards

Daniell

Gregory

1997

Planta

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“…This reaction also plays an important role in regulating the addition of methyl groups. 20 For the rest of the amino acids, the request for them during 11−21 DAF might be satisfied by the degradation of stocking and other proteins. 16 Two identified enzymes, type IIIa membrane protein cp-wap13 (no.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Identification and Roles of Proteins for Seed Development in Mungbean (Vigna radiata L.) Seed Proteomes

Kazłowski

Chen

Chao

et al. 2013

J. Agric. Food Chem.

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Proteomic analysis of developing mungbean (Vigna radiata L.) seeds has not yet been investigated in detail. Fifty-seven proteins were separated by 2-DE, identified by nanoelectrospray mass spectrometry from the present protein databases, and categorized according to their functions. Many of the identified enzymes were involved in central carbon metabolism; thus, a pathway illustrating starch synthesis/breakdown, sugar conversion for glycolysis, and tricarboxylic acid (TCA) cycle was proposed. Quantitative comparison of the protein expression revealed that during developmental process (11-21 days after flowering, DAF), proteins involved in glycolysis, TCA cycle, and alcoholic fermentation showed a trend to be down-regulated, whereas storage proteins were generally up-regulated. The downward tendency of central carbon metabolic proteins suggests a reduction in ATP and oxygen consumption associated with accumulation of storage compounds. UDP-glucose-1-pyrophosphorylase, an upstream enzyme in the starch ADP-Glc pathway, was found as a stably expressed protein throughout the growth stage, demonstrating its importance in mungbean starch biosynthesis. The temporal expression of metabolic enzymes suggests the coordination of an acclimation mechanism and cellular processes associated with accumulation of storage compounds in seed development.

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“…A cDNA library from Lupinus luteus roots with young and ma ture nod ules (Sikorski et al, 1999) was screened with a probe pre pared by PCR am plifi ca tion. Two PCR prim ers: RJ1F 5¢-GTY -ACCAAGAGCAAGTTTGA-3¢ and RJ1R 5¢-AC AAT GGCATTGTTCTTCAT-3¢ were de signed us ing the aligned se quences of SAHases from Catharanthus roseus (Schroder et al, 1994), Medicago sativa (Abrahams et al, 1995), Nicotiana sylvestris (Mitsui et al ., 1993), and Petroselinum crispum (Kawalleck et al, 1992) (see Fig. 2 for lo cal iza tion of prim ers and Fig.…”

Section: Screening Of Cdna LI Brarymentioning

confidence: 99%

Sequence determination and analysis of S-adenosyl-L-homocysteine hydrolase from yellow lupine (Lupinus luteus).

Brzeziński

Janowski²,

Podkowiński³

et al. 2001

Acta Biochim Pol

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The coding sequences of two S-adenosyl-L-homocysteine hydrolases (SAHases) were identified in yellow lupine by screenig of a cDNA library. One of them, corresponding to the complete protein, was sequenced and compared with 52 other SAHase sequences. Phylogenetic analysis of these proteins identified three groups of the enzymes. Group A comprises only bacterial sequences. Group B is subdivided into two subgroups, one of which (B1) is formed by animal sequences. Subgroup B2 consist of two distinct clusters, B2a and B2b. Cluster B2b comprises all known plant sequences, including the yellow lupine enzyme, which are distinguished by a 50-residue insert. Group C is heterogeneous and contains SAHases from Archaea as well as a new class of animal enzymes, distinctly different from those in group B1.

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cDNA for S-Adenosyl-L-Homocysteine Hydrolase from Catharanthus roseus

Cited by 13 publications

References 3 publications

Methylation reactions and the phytoalexin response in alfalfa suspension cultures

Methylation reactions and the phytoalexin response in alfalfa suspension cultures

Identification and Roles of Proteins for Seed Development in Mungbean (Vigna radiata L.) Seed Proteomes

Sequence determination and analysis of S-adenosyl-L-homocysteine hydrolase from yellow lupine (Lupinus luteus).

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