cDNA Cloning of Viroid-Induced Tomato Pathogenesis-Related Protein P23 (Characterization as a Vacuolar Antifungal Factor)

Rodrigo, Ismael; Vera, Pablo; Tornero, Pablo; Hernández-Yago, José; Conejero, Vicente

doi:10.1104/pp.102.3.939

Cited by 58 publications

(46 citation statements)

References 19 publications

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“…3A; P , 0.001), confirming that the expression of this gene is influenced by the def-1 mutation. The transcript levels of the SA-related gene PR-P23 (Rodrigo et al, 1993), were different in def-1 and wild type ( Fig. 3B; P 5 0.0013), with the largest difference on day 4 (Fisher post hoc P 5 0.01).…”

Section: Spider Mite Eggs Produced On Def-1 Are More Viablementioning

confidence: 95%

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

et al. 2004

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The tomato (Lycopersicon esculentum) mutant def-1, which is deficient in induced jasmonic acid (JA) accumulation upon wounding or herbivory, was used to study the role of JA in the direct and indirect defense responses to phytophagous mites (Tetranychus urticae). In contrast to earlier reports, spider mites laid as many eggs and caused as much damage on def-1 as on wild-type plants, even though def-1 lacked induction of proteinase inhibitor activity. However, the hatching-rate of eggs on def-1 was significantly higher, suggesting that JA-dependent direct defenses enhanced egg mortality or increased the time needed for embryonic development. As to gene expression, def-1 had lower levels of JA-related transcripts but higher levels of salicylic acid (SA) related transcripts after 1 d of spider mite infestation. Furthermore, the indirect defense response was absent in def-1, since the five typical spider mite-induced tomato-volatiles (methyl salicylate [MeSA], 4,8,12-trimethyltrideca-1,3,7,11-tetraene [TMTT], linalool, trans-nerolidol, and trans-b-ocimene) were not induced and the predatory mite Phytoseiulus persimilis did not discriminate between infested and uninfested def-1 tomatoes as it did with wild-type tomatoes. Similarly, the expression of the MeSA biosynthetic gene salicylic acid methyltransferase (SAMT) was induced by spider mites in wild type but not in def-1. Exogenous application of JA to def-1 induced the accumulation of SAMT and putative geranylgeranyl diphosphate synthase transcripts and restored MeSA-and TMTT-emission upon herbivory. JA is therefore necessary to induce the enzymatic conversion of SA into MeSA. We conclude that JA is essential for establishing the spider mite-induced indirect defense response in tomato.

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Section: Spider Mite Eggs Produced On Def-1 Are More Viablementioning

confidence: 95%

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

et al. 2004

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“…Protein extracts of tomato leaves were performed by homogenization in extraction buffer (50 mM Tris-HCl, pH 7.5, 15 mM 2-mercaptoethanol), as described in (Rodrigo et al, 1993). Proteins were separated by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 as described by Conejero and Semancik (Conejero and Semancik, 1977).…”

Section: Protein Extraction and Electrophoretic Analysismentioning

confidence: 99%

Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens

Campos

Granell

Tárraga

et al. 2014

Plant Physiology and Biochemistry

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ElsevierCampos Beneyto, L.; Granell Albert, P.; Tarraga Herrero, S.; López Gresa, MP.; Conejero Tomás, V.; Belles Albert, JM.; Rodrigo Bravo, I.... (2014) AbstractWe have observed that treatments with salicylic acid (SA) or gentisic acid (GA) induced resistance to RNA pathogens such as ToMV and CEVd in tomato and Gynura auriantiaca, respectively. Accumulation of SA and GA has been found to occur in plants infected by these pathogens, thus pointing out a possible defence role of both molecules. To study the molecular basis of the observed induced resistance to RNA pathogens the induction of silencing-related genes by SA and GA was considered. For that purpose, we searched for tomato genes which were orthologous to those described in Arabidopsis thaliana, such as AtDCL1, AtDCL2, AtDCL4, AtRDR1, AtRDR2 and AtRDR6, and we tracked their induction in tomato along virus and viroid infections. We observed that CEVd significantly induced all these genes in tomato, with the exception of ToRDR6, being the induction of ToDCL4 the most outstanding. Regarding the ToMV asymptomatic infection, with the exception of ToRDR2, we observed a significant induction of all the indicated silencing-related genes, being ToDCL2 the most induced gene. Subsequently, we analyzed their transcriptional activation by SA and at the time when ToMV was inoculated on plants. ToDCL2, ToRDR1 and ToRDR2 were significantly induced by both SA and GA, whereas ToDCL1 was only induced by SA. Such an induction resulted more effective by SA treatment, which is in agreement with the stronger SA-induced resistance observed. Our results suggest that the observed delay in the RNA pathogen accumulation could be due to the pre-induction of RNA silencing-related genes by SA or GA. Highlights-Treatments with salicylic acid or gentisic acid induce resistance to RNA pathogens -Induction of tomato silencing-related genes by RNA pathogen infections is studied -Induction of RNA silencing by SA or GA treatments is analysed in tomato plants -New connexions between RNA silencing and the SA or GA response are proposed

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“…Protein extracts of tomato leaves were performed by homogenization in acidic extraction buffer (84 mM citric acid, 32 mM sodium phosphate, pH 2.8) as described in Rodrigo et al (1993). Proteins were separated by SDS-PAGE and stained with Coomassie Brilliant Blue R-250 following the method described by Conejero and Semancik (1977).…”

Section: Protein Analysismentioning

confidence: 99%

A Novel Function for the Cathepsin D Inhibitor in Tomato

2006

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Proteinaceous aspartic proteinase inhibitors are rare in nature and are described in only a few plant species. One of them corresponds to a family of cathepsin D inhibitors (CDIs) described in potato (Solanum tuberosum), involving up to 15 isoforms with a high sequence similarity. In this work, we describe a tomato (Solanum lycopersicum) wound-inducible protein called jasmonic-induced protein 21 (JIP21). Sequence analysis of its cDNA predicted a putative function as a CDI. The JIP21 gene, whose protein has been demonstrated to be glycosylated, is constitutively expressed in flowers, stem, and fruit, and is inducible to high levels by wounding and methyl jasmonate in leaves of tomato plants. The genomic sequence of JIP21 shows that the gene is intronless and reveals the presence of both a methyl jasmonate box (TGACT) and a G-box (CACGT) in the promoter. In contrast to the presumed role of JIP21 based on sequence analysis, a detailed biochemical characterization of the purified protein uncovers a different function as a strong chymotrypsin inhibitor, which questions the previously predicted inhibitory activity against aspartic proteinases. Moreover, Egyptian cotton worm (Spodoptera littoralis) larvae fed on transgenic tomato plants overexpressing JIP21 present an increase in mortality and a delay in growth when compared with larvae fed on wild-type plants. These larvae belong to the Lepidoptera family whose main digestive enzymes have been described as being Ser proteases. All these results support the notion that tomato JIP21 should be considered as a chymotrypsin inhibitor belonging to the Ser proteinase inhibitors rather than a CDI. Therefore, we propose to name this protein tomato chymotrypsin inhibitor 21 (TCI21).

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cDNA Cloning of Viroid-Induced Tomato Pathogenesis-Related Protein P23 (Characterization as a Vacuolar Antifungal Factor)

Cited by 58 publications

References 19 publications

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

Jasmonic Acid Is a Key Regulator of Spider Mite-Induced Volatile Terpenoid and Methyl Salicylate Emission in Tomato

Salicylic acid and gentisic acid induce RNA silencing-related genes and plant resistance to RNA pathogens

A Novel Function for the Cathepsin D Inhibitor in Tomato

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