cDNA cloning of IL-1α and IL-1β from mRNA of U937 cell line

Nishida, Tsutomu; Nishino, Norikazu; Takano, Masaaki; Kawai, Kumi; Bando, Keiko; Masui, Yoshihiro; Nakai, Satoru; Hirai, Yoshikatsu

doi:10.1016/0006-291x(87)90671-1

Cited by 115 publications

(36 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The purity of these preparations is >99.9%, and the endotoxin levels are 4 0 pg/mg of protein. Recombinant human IL-lP (2 x lo7 units/mg of protein) was obtained from Otsuka Pharmaceutical (Osaka, Japan) (20). The purity of this material is at least 99%, and the endotoxin level is 100 pgimg of protein.…”

Section: Methodsmentioning

confidence: 99%

Interleukin‐1–inhibitory IgG in sera from some patients with rheumatoid arthritis

Suzuki

Akama

Okane

et al. 1989

Arthritis & Rheumatism

View full text Add to dashboard Cite

Inhibition of interleukin‐1°aL (IL‐1°aL) activity was detected in 7 of 41 serum samples from patients with rheumatoid arthritis (RA). These 7 sera inhibited not only IL‐1°aL–induced endothelial cell adherence to neutrophils, but also IL‐1°bT–induced endothelial cell adherence, although to a lesser extent. These sera showed no influence on tumor necrosis factor–induced endothelial cell adherence. No inhibitory activity was found in 40 sera from normal control subjects. Studies to further examine these effects included gel filtration analysis of 2 of the RA sera. The inhibitory activity was eluted near Mr 158 kd and above Mr 250 kd. Analysis by protein A affinity chromatography showed that IL‐1–inhibitory activity was present in protein A–binding fractions. Purified IgG (by DE‐52 column chromatography) from RA patients was found to be as potent an inhibitor as the protein A–binding fractions, which suggests that the major inhibitory activity in RA sera is attributable to IgG molecules. These purified IgG molecules also inhibited IL‐1–induced proliferation of mouse thymocytes but did not influence IL‐2–dependent proliferation of the CTLL‐2 murine T cell line. The 7 patients whose sera showed IL‐1–inhibitory activity had mild RA and low titers of rheumatoid factor. The findings, taken together, suggest a possible regulatory role of IL‐1–inhibitory IgG in RA disease activity.

show abstract

Section: Methodsmentioning

confidence: 99%

Interleukin‐1–inhibitory IgG in sera from some patients with rheumatoid arthritis

Suzuki

Akama

Okane

et al. 1989

Arthritis & Rheumatism

View full text Add to dashboard Cite

show abstract

“…Japan). Human rlL-l/( [22] was kindly supplied by Dr Y. Hirai (Otsuka Pharmaceutical Co., Tokushima, Japan). None of these materials contained endotoxins (sensitivity limit 01 ng/ml).…”

Section: Sulyjectsmentioning

confidence: 99%

“…ng/ml. 20 pg/ml and 20 pg/ml, respectively) were performed essentially as described previously [22,23]. Briefly, microtitration plates (Nunc, Napervillc, IL) were coated with anti-IL-6, anti-IL-I/^ or anli-TNF-x MoAb (10 /(g/ml) in 100 /il/well of PBS.…”

Section: Enzyme Immunoassays { Eiasj Of Human Il-6 Il-lfi Und Tnf-ctmentioning

confidence: 99%

IL-6 in malignant pleural effusions and its augmentation by intrapleural instillation of IL-2

Yanagawa

Sone

Munekata

et al. 1992

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe levels and activities of endogenous IL-6 in malignant pleura! effusions due to lung cancer before and during daily intrapleural instillations of reeombinant IL-2 were examined by enzyme immunoassay and bioassay using an IL-6-dependenl murine hybridoma cell line MH60.BSF2. Before therapy, malignant pieural effusions contained various levels of IL-6. Daily intrapteural instillation of recombinani lL-2 for treatment of malignant pleurisy resulted in significant augmentation of the levels and activities of IL-6 in the pleura! effusions. On fractionation of the pieural effusion by chromatography. one major peak ofmateria! with a mo!, wi of 24 k D showed lL-6 activity. In contrast, no significant level of tumour necrosis factor-alpha or IL-I/J was delectable in pieural effusions before or during local IL-2 therapy. These data suggest that IL-2 is an important regulatory factor of secondary IL-6 producUoti.

show abstract

“…GM-CSF was detected using a Pstl-NcoI fragment (0.5 kb) of GM-CSF cDNA derived from pGM-CSF 22-25 isolated from PHA and PMA stimulated human tonsillar cDNA library (unpublished). IL-1,8 was detected using a PstI-PvulI fragment (0.7 kb) of IL-I~' cDNA from pcD-G1F-16 [35]. ~'-Actin DNA probe was purchased from Wako Pure Chemical Industries Ltd.…”

Section: Dna Probesmentioning

confidence: 99%

Dexamethasone regulation of the expression of cytokine mRNAs induced by interleukin‐1 in the astrocytoma cell line U373MG

Nishida¹,

Nakai²,

Kawakami³

et al. 1989

FEBS Letters

Self Cite

View full text Add to dashboard Cite

BSF-2/IL-6, GM-CSF and IL-lfl mRNAs were induced by recombinant IL-I in human astrocytoma cell line U373MG. The induction of BSF-2/IL-6 and IL-lfl mRNAs did not require de novo protein synthesis while that of GM-CSF mRNA required a newly synthesized protein. Dexamethasone inhibited the induction of these cytokine mRNAs by IL-I. This process seems to require continued protein synthesis. These results suggest that the production of these cytokines are positively and negatively controlled by IL-1 and glucocorticoids, respectively, in astrocytes.

show abstract

cDNA cloning of IL-1α and IL-1β from mRNA of U937 cell line

Cited by 115 publications

References 19 publications

Interleukin‐1–inhibitory IgG in sera from some patients with rheumatoid arthritis

Interleukin‐1–inhibitory IgG in sera from some patients with rheumatoid arthritis

IL-6 in malignant pleural effusions and its augmentation by intrapleural instillation of IL-2

Dexamethasone regulation of the expression of cytokine mRNAs induced by interleukin‐1 in the astrocytoma cell line U373MG

Contact Info

Product

Resources

About