cDNA cloning, expression and characterization of an allergenic L3 ribosomal protein of <i>Aspergillus fumigatus</i>

Saxena, Shipra; Madan, Taruna; Muralidhar, K.; Sarma, P. Usha

doi:10.1046/j.1365-2249.2003.02257.x

Cited by 16 publications

(10 citation statements)

References 29 publications

(29 reference statements)

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“…The ability of P1 to stimulate histamine release from ABPA patients and cytokines of Th2 type, although at a lower magnitude than SDA, suggests that it could be further explored for intradermal skin testing. There have been numerous reports of diagnostically relevant native and recombinant antigens for serodiagnosis of aspergillosis (12,14,15,16,19,25,28,30). The reported diagnostic sensitivity and specificity vary greatly among the different reports, even for similar antigen preparations.…”

Section: Discussionmentioning

confidence: 99%

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

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Allergic bronchopulmonary aspergillosis (ABPA) is an immunologically complex allergic disorder caused by the fungal pathogen Aspergillus fumigatus. Elevated levels of total immunoglobulin E (IgE), specific IgE, and IgG antibodies in sera are important immunodiagnostic criteria for ABPA. International reference standards or standardized immunodiagnostic assays are not available due to a lack of well-defined diagnostic antigens. The present study was carried out to identify and evaluate the immunodiagnostic relevance of synthetic epitopic peptides of Asp f 1, a major allergen, antigen, or cytotoxin of A. fumigatus. Five overlapping peptides were synthesized from the N terminus of Asp f 1, one of the potential immunodominant regions predicted by algorithmic programs. The 11-amino-acid synthetic peptide (P1) significantly inhibited both IgG binding (89.10% ؎ 4.45%) and IgE binding (77.32% ؎ 3.38%) of the standardized diagnostic antigen (SDA) (a well-defined pool of diagnostically relevant allergens and antigens of A. fumigatus). With a panel of sera of ABPA patients, allergic patients with skin test negativity to A. fumigatus, and healthy individuals, P1 showed a higher diagnostic efficiency than SDA (specific IgG, 100%; specific IgE, 98.3%). The diagnostic efficiency of P1 could be attributed to the presence of homologous epitopes in various immunodominant allergens or antigens of A. fumigatus. The ability of P1 to induce histamine release from sensitized mast cells and a Th2 type of cytokine profile in peripheral blood mononuclear cells of ABPA patients suggests its potential for use in intradermal testing. P1 could be further explored for development of a standardized, specific, and sensitive immunodiagnostic test for aspergillosis.

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Section: Discussionmentioning

confidence: 99%

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Madan

Priyadarsiny

Vaid

et al. 2004

Clin Vaccine Immunol

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“…High throughput screening of a human lung cDNA library displayed on phage surface [31, 32] revealed more than 150 partial and complete cDNA sequences potentially encoding IgE-binding proteins. Although not yet analyzed in detail, some of the cDNA sequences, like those encoding α-tubulin [33] or ribosomal L 3 protein [34], show a high degree of sequence identity and cross-reactivity to environmental allergens [Zeller et al, unpublished results]. In these cases, it makes sense to assume that IgE autoreactivity can be traced back to common structural features shared between environmental and self antigens (see below).…”

Section: Ige-binding Self Antigensmentioning

confidence: 99%

Immunoglobulin-E-Mediated Reactivity to Self Antigens: A Controversial Issue

Zeller¹,

Glaser²,

Vilhelmsson

et al. 2007

Int Arch Allergy Immunol

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Immunoglobulin E (IgE) reactivity to self antigens is well established in vitro by ELISA, inhibition ELISA, Western blot analyses and T cell proliferation experiments. In vivo, IgE-binding self antigens are able to elicit strong type I reactions in sensitized individuals and, in the case of human manganese superoxide dismutase, to elicit eczematous reactions on healthy skin areas of patients suffering from atopic eczema. The reactions against self antigens sharing structural homology with environmental allergens can be plausibly explained by molecular mimicry between common B cell epitopes. For the second class of IgE-binding self antigens without sequence homology to known allergens, it is still unclear if the structures are able to induce a B cell switch to IgE production, or if the reactivity is due to sequence similarity shared with not yet detected environmental allergens. However, in all cases, cross-reactivity is never complete, indicating either a lower affinity of IgE antibodies to self allergens than to the homologous environmental allergens or the presence of additional B cell epitopes on the surface of the environmental allergens, or both. Increasing evidence shows that self allergens could play a decisive role in the exacerbation of long-lasting atopic diseases. However, the only observation supporting a clinical role of IgE-mediated autoreactivity is confined to the fact that IgE levels against self antigens correlate with disease severity.

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“…Molecular biology techniques were previously used for the identification of Ags and allergens from various fungal species such as Aspergillus fumigatus and Fusarium culmorum (16,17). In analogy to these studies, we now searched a newly developed M. mycetomatis cDNA expression library for immunodominant Ags.…”

Section: Translationally Controlled Tumor Protein From Madurella Mycementioning

confidence: 99%

Translationally Controlled Tumor Protein from Madurella mycetomatis, a Marker for Tumorous Mycetoma Progression

Sande

Janse

Hira

et al. 2006

The Journal of Immunology

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About 40 years ago Abs against the fungus Madurella mycetomatis were first demonstrated to be present in eumycetoma patients, a disease characterized by tumorous swellings. To date nothing is known about the individual immunoreactive Ags present in this fungus. In the present study, we identify its first immunogenic Ag, a protein homologous to the translationally controlled tumor protein (TCTP), a well-conserved histamine release factor in a range of eukaryotes. The gene for this Ag was demonstrated to be present in two variants in M. mycetomatis, with 13% aa difference between the two proteins encoded. In vitro, TCTP was secreted into the culture medium. In vivo, it was found to be expressed on hyphae present in developing stages of the eumycetoma-characteristic black grain. Significant IgG and IgM immune responses, against the whole protein and selected M. mycetomatis-specific peptides, were determined. The Ab levels correlated with lesion size and disease duration. Overall, the patients with the largest lesions had the highest Ab level, which lowered with decreasing size of the lesion. After 6–15 years of disease duration the Ab levels were the highest. TCTP is the first well-characterized immunogenic Ag, simultaneously the first monomolecular vaccine candidate, identified for the fungus M. mycetomatis.

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cDNA cloning, expression and characterization of an allergenic L3 ribosomal protein of Aspergillus fumigatus

Cited by 16 publications

References 29 publications

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Use of a Synthetic Peptide Epitope of Asp f 1, a Major Allergen or Antigen ofAspergillus fumigatus, for Improved Immunodiagnosis of Allergic Bronchopulmonary Aspergillosis

Immunoglobulin-E-Mediated Reactivity to Self Antigens: A Controversial Issue

Translationally Controlled Tumor Protein from Madurella mycetomatis, a Marker for Tumorous Mycetoma Progression

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