cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor

Wada, Etsuko; Way, James M.; Shapira, Hagit; Kusano, Kazuo; Lebacq-Verheyden, Anne Marie; Coy, David H.; Jensen, Robert T.; Battey, James F.

doi:10.1016/0896-6273(91)90250-4

Cited by 280 publications

(179 citation statements)

References 33 publications

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“…Low stringency hybridization was performed overnight at 37°C as previously described [24]. Filters were washed three times at room temperature for 15 min in 1 x SSC, 0.1% SDS and then at 37°C for 20 min in 0.1 x SSC, 0.1% SDS, dried and autoradiographed.…”

Section: Cloning Of Xenopus G and Gmentioning

confidence: 99%

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Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

et al. 1994

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G-proteins of the q family have been implicated as mediators of bombesin receptors action. We cloned Xenopus G, and G,,, and specifically disrupted the synthesis of either protein with selective antisense oligonucleotides. CC14 antisense inhibited responses mediated by neuromedin B receptor (NMB-R) by 74%, though not by gastrin-releasing peptide receptor (GRP-R). G,,, antisense had little effect on either GRP-R-or NMB-R-mediated responses. This suggests that NMB-R couples to G,_, and that GRP-R and NMB-R show distinct G-protein coupling preferences in the Xenopus oocyte.

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Section: Cloning Of Xenopus G and Gmentioning

confidence: 99%

“…RNA samples (10 pg of total RNA) were resolved by electrophoresis on agarose/formaldehyde gel and blotted onto nitrocellulose membranes using standard methodology [24]. Filters were hybridized to "P-labeled Xenopus oocyte GW and G,,, cDNA probes as described previously [24].…”

Section: Rna Analysismentioning

confidence: 99%

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

et al. 1994

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“…(figura 1) O GRP e a neuromedina B mostraram ter efeitos similares àqueles descritos para a bombesina, diferindo na potência relativa em função do efeito estudado. Assim, apresentam um largo espectro de atividades biológicas e farmacológicas, podendo atuar na termo e glicorregulação (7), na regulação do comportamento (8), e na regulação de secreções endócrinas e exócrinas (9,10). Além disso, em alguns modelos de culturas de células, esses peptídeos bombesina-similes parecem funcionar como fatores de crescimento autócrinos (11).…”

Section: Bombesina E Seus Análogos Em Mamíferosunclassified

“…Os receptores para GRP, neuromedina B e o BRS-3 são proteínas de membrana plasmática que apresentam homologia de cerca de 50% entre si (8,17,18). Esses receptores interagem com proteínas G e ativam a liberação de inositol-trifosfato (16,(19)(20)(21), seu mensageiro intracelular parece ser o cálcio iônico (22).…”

Section: Distribuição Tecidual Dos Receptores Para Bombesinaunclassified

Peptídeos bombesina-símiles: novos reguladores da secreção adeno-hipofisária

Ortiga-Carvalho

Pazos‐Moura

2000

Arq Bras Endocrinol Metab

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A neuromedina B (NB) e o peptídeo liberador de gastrina são peptídeos bombesina-símiles encontrados em mamíferos, inclusive em seres humanos. Ambos inibem a secreção hipofisária de tireotrofina (TSH); entretanto, somente a NB tem importância fisiológica demonstrada. A NB é produzida em abundância em tireotrofos e parece inibir a secreção de TSH por via autócrina, uma vez que o bloqueio do peptídeo endógeno causa aumento na liberação do TSH, tanto in vivo quanto in vitro. A NB é positivamente regulada pelos hormônios tireóideos (HT). Os HT aumentam o conteúdo de neuromedina B e do seu RNAm em adeno-hipófises de ratos hipotireóideos, poucas horas após sua administração, o que coincide com diminuição do TSH sérico. Isto nos levou a sugerir que a NB possa ser um intermediário protéico envolvido na inibição aguda da liberação de TSH induzida pelos HT. O TRH também altera rapidamente a expressão da NB. Quinze e 30 minutos após a administração do TRH em ratos normais já há diminuição do conteúdo hipofisário de NB e dos níveis do seu RNAm. No jejum e diabetes experimental, que se caracterizam por diminuição de HT séricos com níveis inadequadamente normais ou diminuídos de TSH, ocorre aumento do conteúdo de NB e de seu RNAm. O análogo de somatostatina, octreotide, também é capaz de aumentar o conteúdo de NB. Assim, a neuromedina B é um importante inibidor local da secreção de TSH, podendo ser uma via final comum de hormônios e neuro-hormônios que determinam variações na secreção de TSH. ABSTRACTNeuromedin B (NB) and gastrin-releasing peptide (GRP) are mammalian bombesin-like peptides. Both peptides have inhibitory effects on thyrotropin (TSH) secretion, however NB is the one that proved to be physiologically relevant. The administration of NB reduces serum TSH of eu-and hypothyroid rats, while the antiserum against NB, that blocks the endogenous peptide, increases TSH secretion. NB is abundantly produced in thyrotrophs and acts locally as a autocrine factor to inhibit TSH release since isolated pituitaries increase the release of TSH in the presence of the specific antiserum. Thyroid hormones (TH) up-regulate pituitary NB peptide and mRNA content. This is a rapid effect: in hypothyroid rats it is seen within a few hours after a single injection of TH, and coincides with TSH suppression. These results suggest that NB could be an intermediary factor in the acute suppressive effect of TH on TSH secretion. TRH also rapidly changes NB expression. In normal rats, 15 to 30 minutes after TRH administration the pituitary NB peptide and mRNA content are already decreased. In experimental fasting and diabetes mellitus, which are characterized by inappropriately normal or decreased serum TSH, both the NB peptide and its mRNA pituitary content are increased. Octreotide, a somatostatin analog, also is able to increase pituitary neu-

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“…Although BN itself is not present in mammals, gastrin-releasing peptide (GRP) and neuromedin B (NMB) are two homologs that have been isolated from mammalian tissue and their receptors cloned and characterized (2,31,37). Structural and pharmacological differences have led to the identification of two BN receptor subtypes referred to as BB2 or GRP-preferring (GRP-R) and BB1 or NMB-preferring (NMB-R) (36).…”

Section: Introductionmentioning

confidence: 99%

Capsaicin treatment differentially affects feeding suppression by bombesin-like peptides

Ladenheim

Knipp

2007

Physiology & Behavior

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Peripheral administration of bombesin (BN) and the related mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB), suppress food intake in rats. To examine whether all BNlike peptides utilize the same neural pathways to reduce feeding, rats were treated on postnatal day 2 with the injection vehicle or capsaicin, a neurotoxin that damages a subset of visceral afferent fibers. When rats reached adulthood, we compared the ability of a dose range of systemically administered BN, GRP18-27 and NMB to reduce intake of a 0.5 kcal/ml glucose solution in a short-term feeding test. Our results demonstrate that capsaicin treatment abolished or attenuated the suppression of glucose intake produced by BN and NMB but had no effect on the ability of GRP to reduce feeding. These results suggest that different neural substrates underlie the anorexic effects of peripherally administered BN-like peptides.

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cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor

Cited by 280 publications

References 33 publications

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

Peptídeos bombesina-símiles: novos reguladores da secreção adeno-hipofisária

Capsaicin treatment differentially affects feeding suppression by bombesin-like peptides

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cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor

Cited by 280 publications

References 33 publications

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to Gαq and not Gα11

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to Gαq and not Gα11

Peptídeos bombesina-símiles: novos reguladores da secreção adeno-hipofisária

Capsaicin treatment differentially affects feeding suppression by bombesin-like peptides

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Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11

Neuromedin B receptor, expressed in Xenopus laevis oocytes, selectively couples to G_αq and not G_α11