Abstract:We isolated a cDNA encoding the rat membrane-associated 65167-kDa calcium-binding protein, CBP 65/67, from a A ZAP I1 cDNA-expression library of rat liver by immunoscreening using monospecific polyclonal anti-(CBP 65/67) antibodies and monoclonal anti-(CBP 65/67) IgG. The product of this cDNA expressed in Escherichia coli was confirmed as CBP 65167 both by immunostaining and by comparison of the molecular mass with the CBP 65/67 isolated from rat liver by SDSPAGE. The cDNA sequence and the deduced amino acid s… Show more
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