cDNA cloning and expression analysis of the myosin heavy chain (MYH) gene of the mandarin fishSiniperca kneri

Abstract

In this study, we applied RT-PCR and cDNA cloning techniques to clone myosin heavy chain (MYH) cDNA from muscle tissues of the mandarin ¢sh Siniperca kneri. The cDNA was determined to be of 6987 base pairs in length, encoding a peptide of 1937 amino acids (Genbank accession no. EF446616). A search of encoded protein sequences in the NCBI conserved domain database indicated the presence of all known protein domains for MYH proteins, i.e. the myosin motor domain in the N-terminal region, the DIL domain at the C-terminus, and the ATPase domain. The MYH gene and its protein were expressed predominantly in muscle tissues and weakly in cardiac tissues. Developmentally, the MYH gene was ¢rst expressed in the muscle formation stage and continued later on. Our work provided a novel mypsin heavy chain gene sequence in ¢sh biology and the results indicate that the MYH gene and the protein it encodes are important for the growth and development of the mandarin ¢sh, as well as its muscle characterization.

Expression and functional analyses of two essential myosin light chains from the fast white muscle of the spotted mandarin fish Siniperca scherzeri

“…When we compared MLC1 to MLC3, MLC1 was expressed a few hours earlier, making the MLC1 gene a very early and sensitive marker for fast skeletal muscle differentiation in the spotted mandarin fish. Previously, we applied RT-PCR techniques to detect expression patterns of the MyHC and MLCs in Siniperca kneri and Siniperca chuatsi, respectively [16,17]. We demonstrated that the S. kneri MyHC gene was expressed from the muscle formation stage onwards.…”

“…We decided to use CMF species as a model to study genes involved in controlling muscle flesh quality and muscle structural characteristics. Thus, we assayed CMF skeletal muscle transcriptional profile by cDNA microarray analysis and cDNA-EST sequencing [14,15] and cloned and characterized several muscle structural genes, including myosin heavy chain (MyHC) and myosin light chain (MLC) genes [16,17]. However, we did not observe any available MLC sequence for S. scherzeri and the characterization of these genes had not been performed.…”

Gene structure, recombinant expression and function characterization of Siniperca chuatsi Fsrp‐3

“…Therefore, an understanding of muscle growth and development in important aquaculture species could be beneficial for humans (Zhu et al , ). The Chinese mandarin fish Siniperca chuatsi (Basilewsky 1855) is one of the most commercially valuable species in China and other Asian countries because of its high nutritional value and palatability (Zhang et al , ). Previous studies have revealed that recombinant fish Fst successfully inhibits Mstn activity and enhances muscle mass in gilthead sea bream Sparus aurata L. 1758 (Bruria et al , ; Rebhan & Funkenstein, ).…”

Muscle Fiber Differentiation and Growth Patterns During Hyperplasia and Hypertrophy in the Ricefield Eel Regulated by Myogenic Regulatory Factors

“…Hypertrophy is the process of an increase in fiber size (Zhang et al. ). Fish growth is mainly contributed by hypertrophy of muscle fibers after the larval stage (Weatherley and Gill ).…”