cDNA cloning and endogenous expression of a flavonoid 3′5′-hydroxylase from petals of lisianthus (Eustoma grandiflorum)

Nielsen, Karen Margrethe; Podivinsky, Ellen

doi:10.1016/s0168-9452(97)00175-1

Cited by 24 publications

(30 citation statements)

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“…2. TG1 shares 98% homology with a lisianthus clone (E. grandi£orum) [8], 77% with a gentian clone (Gentiana tri£ora) [7], 74% with petunia Hf1 [4,17], 73% with petunia Hf2 [4], and 71% with egg plant (Solanum melongena) [5]. Although E. russellianum and E. grandi£orum are not strictly distinguished systematically, we found signi¢cant genetic variation between TG1 and a previously reported lisianthus clone: there are nine substitutions of amino acid residues.…”

Section: Resultsmentioning

confidence: 44%

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Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

et al. 1999

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Flavonoid-3P P,5P P-hydroxylase (F3P P5P PH), a member of the cytochrome P450 family, is the key enzyme in the synthesis of 3P P,5P P-hydroxylated anthocyanins, which are generally required for blue or purple flowers. A full-length cDNA, TG1, was isolated from prairie gentian by heterologous hybridization with a petunia cDNA, AK14, which encodes F3P P5P PH. To investigate the in vivo function of TG1 and AK14, they were subcloned into a plant expression vector and expressed under the control of the CaMV35S promoter in transgenic tobacco or petunia, both of which originally lack the enzyme. Transgenic petunia plants had a dramatic change in flower color from pink to magenta with a high content of 3P P,5P P-hydroxylated anthocyanins. In contrast, transgenic tobacco plants had minimal color change with at most 35% 3P P,5P P-hydroxylated anthocyanin content. These results indicate that the products of TG1 and AK14 have F3P P5P PH activity in planta and that interspecific gene transfer alters anthocyanin pigment synthesis. The difference in apparent F3P P5P PH activity between tobacco and petunia is discussed.z 1999 Federation of European Biochemical Societies.

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Section: Resultsmentioning

confidence: 44%

“…They detected F3P5PH activity in vitro using a yeast expression system but presented a limited transgenic study. Sequences have also been reported for petunia [5], egg plant [6], gentian [7] and lisianthus [8]. Tanaka et al [7] also reported F3P5PH activity using a yeast expression system.…”

Section: Introductionmentioning

confidence: 92%

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

et al. 1999

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“…3,4) as well as by others. [5][6][7][8][9][10][11] Their sequence similarity indicates that they belong to the same gene family of cytochrome P450, CYP75A. Some reviews referred to unpublished results or to the possibility of engineering of anthocyanin composition and ‰ower color with F3?5?H genes.…”

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confidence: 99%

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Okinaka

Shimada

Nakano-Shimada

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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“…For instance, F3'H is supposed to be involved not only in anthocyanin biosynthesis but also in flavone biosynthesis (Kitada et al, 2001). Nielsen and Podivinsky (1997) and Noda et al (2004) did not detect the F3'5'H gene transcript in pink flower lisianthus lines that lack delphinidin but accumulate pelargonidin. Thus, by analogy with F3'5'H, we anticipated marked variation in F3'H expression among the three lisianthus lines with different anthocyanidin compositions; however, such marked variation of F3'H expression was rarely detected among these lines in our RT-PCR analysis (Fig.…”

Section: Transgenic Complementation Of I Nil With Lisianthusmentioning

confidence: 95%

“…In lisianthus, two types of full-length F3'5'H cDNA clones have been isolated from the petals of purple flowers (Nielsen and Podivinsky, 1997;Noda et al, 2004;Shimada et al, 1999); however, the molecular and biochemical characterization of lisianthus F3'H has not been described. Japanese morning glory [Ipomoea nil (L.) Roth.]…”

Section: Introductionmentioning

confidence: 99%

Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H

et al. 2015

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A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar 'Violet', which lacks a functional F3'H gene. In addition, the transgenic 'Violet' plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

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cDNA cloning and endogenous expression of a flavonoid 3′5′-hydroxylase from petals of lisianthus (Eustoma grandiflorum)

Cited by 24 publications

References 13 publications

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>

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cDNA cloning and endogenous expression of a flavonoid 3′5′-hydroxylase from petals of lisianthus (Eustoma grandiflorum)

Cited by 24 publications

References 13 publications

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants1

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants1

Selective Accumulation of Delphinidin Derivatives in Tobacco Using a Putative Flavonoid 3′,5′-Hydroxylase cDNA fromCampanula medium

Cloning of the Flavonoid 3&apos;-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3&apos;H</i>) and Complementation of an F3&apos;H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3&apos;H</i>

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Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Expression of chimeric P450 genes encoding flavonoid‐3′,5′‐hydroxylase in transgenic tobacco and petunia plants¹

Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>