cDNA Cloning and Developmental Alterations in Gene Expression of the Two Pit-1/GHF-1 Transcription Factors in the Chicken Pituitary

Tanaka, Minoru; Yamamoto, Ichiro; Ohkubo, Tadayasu; Wakita, Masaaki; Hoshino, Sadao; Nakashima, Kenichiro

doi:10.1006/gcen.1999.7270

Cited by 35 publications

(26 citation statements)

References 29 publications

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“…Previously, researchers suggested significant roles for Pit-1 in the regulation of PRL, GH and TSH gene expression and secretion in the chicken pituitary [14,17,18]. However, the current study found the induction of Pit-1 in 6 types of glandular cell (GH, TSH, PRL, FSH, LH or ACTH cells).…”

Section: Resultscontrasting

confidence: 75%

“…Furthermore, a significant number of Pit-1 nuclei were positive in the glandular cells including gonadotropes of the rat [11]. In birds, Pit-1 gene has been cloned from turkey and chicken pituitary [12][13][14], and we showed that Pit-1 protein induction was changed with inhibition and resumption of feeding [15]. However, there has been no report that identified the cell types of anterior pituitary containing Pit-1 in birds.…”

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confidence: 83%

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Immunocytochemical Identification of Pit-1 Containing Cells in the Anterior Pituitary of Hens

Chowdhury

Yoshimura

2003

Journal of Reproduction and Development

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Abstract.Our goal was to identify the cells expressing Pit-1 protein in chicken anterior pituitary. The anterior pituitaries were collected from laying hens after perfusion with formalin-PBS, and fixed with Bouin's fixative followed by paraffin embedding. Sections of the anterior pituitaries were immunostained for Pit-1 in the first staining sequence followed by staining for 6 types of pituitary hormones in the second sequence. Pit-1 positive nuclei were observed in the glandular cells in both the cephalic and caudal lobes. Pit-1 immunoreaction products were colocalized in the glandular cells immunopositive for growth hormone, thyroid-stimulating hormone, follicle-stimulating hormone, luteinizing hormone, adrenocorticotropic hormone or prolactin. These results indicate that Pit-1 protein induction occurs in 6 types of glandular cells, suggesting that Pit-1 may regulate hormone synthesis in each glandular cell in the chicken pituitary.

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Section: Resultscontrasting

confidence: 75%

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confidence: 83%

Immunocytochemical Identification of Pit-1 Containing Cells in the Anterior Pituitary of Hens

Chowdhury

Yoshimura

2003

Journal of Reproduction and Development

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“…In the spontaneous mutant Snell dwarf mouse (pit-1 dw ), pit-1 deficiency leads to the ablation of thyrotropes, somatotropes and mammotropes (Li et al 1990;Sornson et al 1996) indicating that somatotropes and mammotropes are descendants of common pit-1-expressing precursor cells. Avian pit-1 has been cloned from turkey and chicken pituitary glands (Wong et al 1992;Tanaka et al 1999). pit-1 mRNA was first detected on E5 in the chicken pituitary gland ( Van et al 2000) and was detected in both cephalic and caudal lobes by Northern blot analysis in the adult chicken (Tanaka et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Avian pit-1 has been cloned from turkey and chicken pituitary glands (Wong et al 1992;Tanaka et al 1999). pit-1 mRNA was first detected on E5 in the chicken pituitary gland ( Van et al 2000) and was detected in both cephalic and caudal lobes by Northern blot analysis in the adult chicken (Tanaka et al 1999). However, little is known about the detail property of spatial appearance of pit-1-producing cells and pit-1 mRNA expression in the embryonic chicken pituitary gland.…”

Section: Introductionmentioning

confidence: 99%

Independent differentiation of mammotropes and somatotropes in the chicken embryonic pituitary gland

Zheng

Nakamura

Maseki

et al. 2005

Histochem Cell Biol

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It has been reported that mammotropes in a rodent pituitary gland are derived from somatotropes via somatomammotropes (SMTs), cells that produce both growth hormone (GH) and prolactin (Prl). However, no studies have been done on the transdifferentiation of somatotropes in the chicken pituitary gland. In this study, in order to determine the origin of mammotropes, we studied detail property of appearance of chicken somatotropes, mammotropes and pit-1 cells and then evaluated the existence of SMTs in the chicken embryonic pituitary gland. Immunohistochemical analysis revealed that GH-immunopositive (GH-ip) cells appeared on embryonic day (E) 14 and were mainly distributed in the caudal lobe, while Prl-immunopositive (Prl-ip) cells appeared in the cephalic lobe of the pituitary gland on E16. In situ hybridization (ISH) and RT-PCR analysis showed that expression of GH and Prl mRNA starts at E12 in the caudal lobe and at E14 in the cephalic lobe respectively. Pit-1 mRNA was first detected on E5 by RT-PCR, and pit-1 mRNA-expressing cells were found in the cephalic lobe on E8. Then with the ontogeny of the chicken, these cells spread into both lobes. Using a double staining method with ISH and immunohistochemistry, we could not detect the existence of SMTs in the chicken embryonic pituitary gland even in the marginal region of either lobe. These results suggest that chicken somatotropes and mammotropes independently appear in different lobes of pituitary gland and that transdifferentiation from somatotropes to mammotropes is not the central route for differentiation of mammotropes in the embryonic chicken pituitary gland.

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“…The pit-1 factor is also involved in development of anterior pituitary gland, silencing adrenarche (Taha et al 2005) and inducing differentiation of hepatic progenitor cells into prolactin-producing cells (Lee et al 2005). The pit-1 cDNA has been identified in a variety of species including chicken (Tanaka et al 1999). The pit-1 gene was comprised of 6 exons in birds and fishes (Yamada et al 1993), which is located on chromosome 1 in chicken (Chicken Genome Browser Gateway).…”

Section: Introductionmentioning

confidence: 99%

Polymorphism at exon 1 of pit-1 gene and its association with immunocompetence traits in layer chicken

Bhattacharya

Priyanka

Chatterjee

et al. 2011

Journal of Applied Animal Research

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A study was designed to explore genetic variability of partial promoter and exon 1 of pit-1 gene and its effect on immune competence traits in White Leghorn chicken. Single stranded conformation polymorphism technique followed by sequencing was employed to detect polymorphism of the gene. Three genotypes (AA, AB and BB) were observed in White Leghorn chicken where two alleles (A and B) were found. AB genotype and A allele were predominant in the population. Different demographic parameters estimated in the White Leghorn chicken lines indicated the presence of genetic divergence among the lines. The pit-1 mRNA expression varied among the genotypes where genotype BB expressed higher than genotype AA and AB. The genotypes as well as alleles showed significant effect (P B 0.05) on sheep RBC titre in White Leghorn chicken.

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cDNA Cloning and Developmental Alterations in Gene Expression of the Two Pit-1/GHF-1 Transcription Factors in the Chicken Pituitary

Cited by 35 publications

References 29 publications

Immunocytochemical Identification of Pit-1 Containing Cells in the Anterior Pituitary of Hens

Immunocytochemical Identification of Pit-1 Containing Cells in the Anterior Pituitary of Hens

Independent differentiation of mammotropes and somatotropes in the chicken embryonic pituitary gland

Polymorphism at exon 1 of pit-1 gene and its association with immunocompetence traits in layer chicken

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