cDNA cloning and complete primary structures of myosin heavy chains from brushtooth lizardfishSaurida undosquamisand wanieso lizardfishS. waniesofast skeletal muscles

Abstract

The complete cDNA sequences encoding predominant types of myosin heavy chain (MYH) in the fast skeletal muscle were determined for brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso, which are used as materials for preparing high-quality surimi-based products. The cDNA consisted of 5973 and 5987 bp, respectively, and both encompassed an open reading frame encoding a polypeptide of 1936 amino acid residues. Brushtooth and wanieso lizardfish MYH showed the amino acid sequence identity of 92-93% to white croaker MYH, which was higher than that of 90% to walleye pollack MYH. The putative binding sites for ATP, actin, and regulatory and essential light chains in the subfragment-1 region of brushtooth lizardfish MYH exhibited a high identity with white croaker counterparts as well as the sequences of subfragment-2 and light meromyosin. In contrast, phylogenetic tree, constructed by the neighbor-joining method based on mitochondrial 16S rRNA gene, revealed that the two lizardfish species formed a cluster with walleye pollack, which was paraphyletic with white croaker. Therefore, a good reputation for lizardfish and white croaker to have a high thermal-gel forming ability seemed to be reflected by MYH rather than biological similarity as revealed by the mitochondrial 16S rRNA gene.KEY WORDS: brushtooth lizardfish, cDNA, fast skeletal muscle, myosin heavy chain, primary structure, wanieso lizardfish.

Three embryonic myosin heavy chain genes encoding different motor domain structures from common carp show distinct expression patterns in cranial muscles

“…3), confirmed that common carp embryonic MYH emb1 and MYH emb2 both belong to a fast type, whereas MYH emb3 does to a slow/cardiac type. MYH emb1 and MYH emb2 formed a monophyletic clade with zebrafish myhz2 and myhc4 (Xu et al, 2000;Peng et al, 2002;Nihei et al, 2006), and these MYHs formed one group with brushtooth lizardfish MYH Su Fast (Hossain et al, 2008), wanieso lizardfish MYH Sw Fast (Hossain et al, 2008), chum salmon MYH Ok Fast (Ojima et al, 1998), walleye pollack MYH Tc Fast (Togashi et al, 2000), yellowbelly rockcod MYH Nc fast (Gauvry et al, 2000), torafugu MYH M743-2 (Ikeda et al, 2004;Ikeda et al, 2007) and white croaker MYH Pa Fast (Yoon et al, 2000) (see Fig. 3).…”

Molecular Mechanisms Involved in Thermally Induced Gel Formation of Japanese Codling Meat Paste during a Two-Step Heating Procedure

“…Based on this background, cDNA encoding a portion of the myosin heavy chain was cloned from fast skeletal muscle of Japanese codling . Phylogenetic analysis of the deduced amino acid sequence of Japanese codling myosin heavy chain revealed that this fish belongs to a group containing white croaker (Pennahia argentatus), walleye pollack (Gadus chalcogrammus), and brushtooth lizardfish (Saurida undosquamis) commonly used as materials for surimi-based products, suggesting that Japanese codling meat is a promising candidate for surimi-based products.…”