cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin

Thomson, Robert K.; Ward, D C; Quaggin, Susan E.; Igarashi, Peter; Muckler, Z. E.; Aronson, Peter S.

doi:10.1006/geno.1998.5402

Cited by 40 publications

(34 citation statements)

References 28 publications

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“…Previous immunohistochemical and Northern hybridization studies have failed to show its expression in other organs including lung, prostate, pancreas, skin, muscle, liver, stomach and intestine. 12 Our findings that all chromophobe RCC and almost all oncocytomas express Ksp-cadherin not only support the theory of distal nephron origin of these renal tumors but also introduce an accurate marker for their diagnosis.…”

Section: Discussionsupporting

confidence: 78%

“…The human and mouse isoforms of Ksp-cadherin were subsequently cloned and were also kidneyspecific. 12 A monoclonal antibody against Kspcadherin is now commercially available. To our knowledge, this is the first report that systematically evaluates the expression of Ksp-cadherin in human kidney tissue and its role in the diagnosis and classification of renal neoplasms.…”

Section: Discussionmentioning

confidence: 99%

“…10,11 Unlike most of the cadherins, which often have a widespread tissue distribution, Ksp-cadherin appears to have unique kidney-specific distribution, and within the kidney, it is observed predominantly in the distal portion of the nephron. 11,12 The expression of Ksp-cadherin in human kidney tissue and renal cell neoplasm has not been evaluated.…”

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confidence: 99%

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Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms

et al. 2005

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Renal cell neoplasms are presumably derived from different cell types of the nephron. Clear cell and papillary renal cell carcinoma (RCC) are thought to be of proximal tubular origin, whereas oncocytoma and chromophobe RCC are derived from intercalated cells of distal nephron. A few molecules, such as RCC marker and CD10, have been shown to be markers for clear cell RCC and papillary RCC. Such markers are not yet available for renal tumors presumably of the distal nephron. The expression of kidney-specific (Ksp) cadherin, a recently cloned gene thought to be transcribed exclusively in the kidney, was studied in normal human kidney, as well as in 105 primary renal neoplasms, including 42 clear cell RCC, 30 papillary RCC, 13 chromophobe RCC, and 20 oncocytomas. The expression patterns were compared with those of RCC marker. The Ksp-cadherin expression was noted preferentially in distal convoluted tubules with a basolateral membrane stain in normal kidney. All 13 chromophobe RCC and 19 of 20 oncocytomas showed diffuse and strong immunoreactivity for Ksp-cadherin, while only 14% clear cell RCC and 13% papillary RCC showed focal positivity. The RCC marker expression was detected in 85%, 98%, 15% and 0% of clear cell RCC, papillary RCC, chromophobe RCC, and oncocytoma, respectively. A few clear cell RCC and papillary RCC showed dual expression of both RCC marker and Kspcadherin, which appear to have distinct histologic features. These results demonstrated high sensitivity and specificity of Ksp-cadherin for distal convoluted tubules, which can be used as adjunct for diagnosis of chromophobe RCC. Modern Pathology (2005) 18, 933-940.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

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Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms

et al. 2005

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“…1A). The Ksp-cadherin gene, which is expressed only in kidney cells, maps to chromosome 16q21-16q22 (19), a region that contains at least seven other cadherin genes (CDH1, CDH3, CDH5, CDH8, CDH11, CDH13, and CDH15). The DNA sequences encoding each of the ectodomains of the Ksp-cadherin gene ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes

Wu¹

2000

Proceedings of the National Academy of Sciences

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Recent studies revealed a striking difference in the genomic organization of classic cadherin genes and one family of ''nonclassic cadherin'' genes designated protocadherins. Specifically, the DNA sequences encoding the ectodomain repeats of classic cadherins are interrupted by multiple introns. By contrast, all of the encoded ectodomains of each member of the protocadherin gene clusters are present in one large exon. To determine whether large ectodomain exons are a general feature of protocadherin genes we have investigated the genomic organization of several additional human protocadherin genes by using DNA sequence information in GenBank. These genes include protocadherin 12 (Pcdh12), an ortholog of the mouse vascular endothelial cadherin-2 gene; hFmi1 and hFmi2, homologs of the Drosophila planar cell polarity gene, flamingo; hFat2, a homolog of the Drosophila tumor suppressor gene fat; and the Drosophila DN-cadherin and DE-cadherin genes. Each of these genes was found to be a member of the protocadherin subfamily, based on amino acid sequence comparisons of their ectodomains. Remarkably, all of these protocadherin genes share a common feature: most of the genomic DNA sequences encoding their ectodomains are not interrupted by an intron. We conclude that the presence of unusually large exons is a characteristic feature of protocadherin genes.

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“…2). The position of the exon-intron boundaries were localized by comparing the genomic sequence of Ksp-cadherin with the corresponding cDNA sequences [37,43]. When in doubt, the general rules for splice site consensus sequences were used to define their EC1-EC7, extracellular cadherin homology repeats; TM, transmembrane domain; CP, cytoplasmic region.…”

Section: Gene Structure Comparison Of Ksp-and Li-cadherinmentioning

confidence: 99%

Unique gene structure and paralogy define the 7D-cadherin family

Wendeler

Jung

Himmelbauer

et al. 2006

Cell. Mol. Life Sci.

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Abstract. Cadherins are Ca 2+ -dependent transmembrane glycoproteins crucial for cell-cell adhesion in vertebrates and invertebrates. Classification of this superfamily due to their phylogenetic relationship is currently restricted to three major subfamilies: classical, desmosomal and protocadherins. Here we report evidence for a common phylogenetic origin of the kidney-specific Ksp-(Cdh16) and the intestine-specific LI-cadherin (Cdh17). Both genes consist of 18 exons and the positions of their exon-intron boundaries as well as their intron phases are perfectly conserved. We found an extensive paralogy of more than 40 megabases in mammals as well as teleost fish species encompassing the Ksp-and LI-cadherin genes. A comparable paralogy was not detected for other cadherin gene loci. These findings suggest that the Ksp-and LI-cadherin genes originated by chromosomal duplication early during vertebrate evolution and support our assumption that both proteins are paralogues within a separate cadherin family that we have termed 7D-cadherins.

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cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin

Cited by 40 publications

References 28 publications

Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms

Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms

Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes

Unique gene structure and paralogy define the 7D-cadherin family

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