cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod,<i>Cypridina noctiluca</i>

Nakajima, Yoshihiro; Kobayashi, Koichi; Yamagishi, Kazutoshi; Enomoto, Toshiteru; Ohmiya, Yoshihiro

doi:10.1271/bbb.68.565

Cited by 104 publications

(104 citation statements)

References 11 publications

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“…5b), values better than those referred to other luciferase, such as Gaussia luciferase, Cypridina noctiluca luciferase and Vargula hilgendorfii luciferase [25][26][27] which evidence negligible activity after 60 min incubation at 65°C.…”

Section: Temperature and Ph Effect On Enzyme Activity And Stabilitymentioning

confidence: 74%

Purification and characterization of a novel thermostable luciferase from Benthosema pterotum

Homaei

Mymandi

Sariri

et al. 2013

Journal of Photochemistry and Photobiology B: Biology

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a b s t r a c tA novel luciferase from Benthosema pterotum, collected from Port of Jask, close to Persian Gulf, was purified for the first time, using Q-Sepharose anion exchange chromatography. The molecular mass of the novel enzyme, measured by SDS-PAGE technique, was about 27 kDa and its K m value is 0.4 lM; both values are similar to those of other coelenterazine luciferases. B. pterotum (BP) luciferase showed maximum intensity of emitted light at 40°C, in 20 mM Tris buffer, pH 9 and 20 mM magnesium concentration. Experimental measurements indicated that BP luciferase is a relatively thermostable enzyme; furthermore it shows a high residual activity at extreme pH values. Its biological activity is strongly inhibited by 1 mM Cu 2+ , Zn 2+ and Ni 2+ , while calcium and mainly magnesium ions strongly increase BP luciferase activity. The B. pterotum luciferase generated blue light with a maximum emission wavelength at 475 nm and showed some similarity with other luciferases, while other parameters appeared quite different, in this way, confirming that a novel protein has been purified.

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Section: Temperature and Ph Effect On Enzyme Activity And Stabilitymentioning

confidence: 74%

Purification and characterization of a novel thermostable luciferase from Benthosema pterotum

Homaei

Mymandi

Sariri

et al. 2013

Journal of Photochemistry and Photobiology B: Biology

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“…To construct the SeV vector, marker genes were inserted into a unique NotI site created in SeV genomic cDNA as described (13). cDNAs encoding enhanced green fluorescent protein (EGFP), Cypridina noctiluca luciferase (CLuc), and fibroblast growth factor 1-proteoglycan fusion protein (PG-FGF-1) were amplified by PCR to add the transcription initiation signal (S) and termination signal (T), using pEGFP-1 (Clontech, Mountain View, CA), pCLm (14), and pMex-RyuII (15) as templates, respectively. The detailed procedure for the construction of the vector cDNA with marker genes is described in supplemental Fig.…”

Section: Methodsmentioning

confidence: 99%

Persistent and Stable Gene Expression by a Cytoplasmic RNA Replicon Based on a Noncytopathic Variant Sendai Virus

Nishimura

Segawa

Goto

et al. 2007

Journal of Biological Chemistry

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Persistent and stable expression of foreign genes has been achieved in mammalian cells by integrating the genes into the host chromosomes. However, this approach has several shortcomings in practical applications. For example, large scale production of protein pharmaceutics frequently requires laborious amplification of the inserted genes to optimize the gene expression. The random chromosomal insertion of exogenous DNA also results occasionally in malignant transformation of normal tissue cells, raising safety concerns in medical applications. Here we report a novel cytoplasmic RNA replicon capable of expressing installed genes stably without chromosome insertion. This system is based on the RNA genome of a noncytopathic variant Sendai virus strain, Cl.151. We found that this variant virus establishes stable symbiosis with host cells by escaping from retinoic acid-inducible gene I-interferon regulatory factor 3-mediated antiviral machinery. Using a cloned genome cDNA of Sendai virus Cl.151, we developed a recombinant RNA installed with exogenous marker genes that was maintained stably in the cytoplasm as a high copy replicon (about 4 ؋ 10 4 copies/cell) without interfering with normal cellular function. Strong expression of the marker genes persisted for more than 6 months in various types of cultured cells and for at least two months in rat colonic mucosa without any apparent side effects. This stable RNA replicon is a potentially valuable genetic platform for various biological applications.

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“…To elucidate how oscillatory secretion is generated during chondrogenesis, it was examined which factors determine secretion activity in prechondrogenic cells. The secretory activity was monitored using a reporter carrying the secreted Cypridina luciferase (CLuc) gene fused to ACTIN promoter (Nakajima et al 2004). It was shown that an inhibitor of mitochondrial oxidative phosphorylation oligomycin suppressed CLuc secretion in prechondrogenic cells (Fig.…”

Section: Cmentioning

confidence: 99%

Extracellular ATP signaling via P2X4 receptor and cAMP/PKA signaling mediate ATP oscillations essential for prechondrogenic condensation

Kwon¹

2012

Journal of Endocrinology

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Prechondrogenic condensation is the most critical process in skeletal patterning. A previous study demonstrated that ATP oscillations driven by Ca 2C oscillations play a critical role in prechondrogenic condensation by inducing oscillatory secretion. However, it remains unknown what mechanisms initiate the Ca 2C -driven ATP oscillations, mediate the link between Ca 2C and ATP oscillations, and then result in oscillatory secretion in chondrogenesis. This study has shown that extracellular ATP signaling was required for both ATP oscillations and prechondrogenic condensation. Among P2 receptors, the P2X 4 receptor revealed the strongest expression level and mediated ATP oscillations in chondrogenesis. Moreover, blockage of P2X 4 activity abrogated not only chondrogenic differentiation but also prechondrogenic condensation. In addition, both ATP oscillations and secretion activity depended on cAMP/PKA signaling but not on K ATP channel activity and PKC or PKG signaling. This study proposes that Ca 2C -driven ATP oscillations essential for prechondrogenic condensation is initiated by extracellular ATP signaling via P2X 4 receptor and is mediated by cAMP/PKA signaling and that cAMP/PKA signaling induces oscillatory secretion to underlie prechondrogenic condensation, in cooperation with Ca 2C and ATP oscillations.

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cDNA Cloning and Characterization of a Secreted Luciferase from the Luminous Japanese Ostracod,Cypridina noctiluca

Cited by 104 publications

References 11 publications

Purification and characterization of a novel thermostable luciferase from Benthosema pterotum

Purification and characterization of a novel thermostable luciferase from Benthosema pterotum

Persistent and Stable Gene Expression by a Cytoplasmic RNA Replicon Based on a Noncytopathic Variant Sendai Virus

Extracellular ATP signaling via P2X4 receptor and cAMP/PKA signaling mediate ATP oscillations essential for prechondrogenic condensation

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