cDNA cloning and analysis of tissue specific genes by differential hybridization screening procedures: analysis of OSF-1 gene specifically expressed in brain and osteoblasts.

Hashimoto, T.

doi:10.1254/fpj.102.47

Cited by 2 publications

(1 citation statement)

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“…The detection of Brk-1/BMPR1a or BMP receptor type 1, (42) procollagens, (43) SPARC, (44) OSF-1, (45) OSF-2/periostin, (46 -48) fibronectin, the fibronectin receptor, (24,25) the fibroblast growth factor (FGF) receptor, (49) and minopontin/ osteopontin (OPN) (50,51) (Tables 1 and 2) agrees well with the previously reported expression of these markers in osteoblast-related cell types. The relative decreases observed in the hybridization signals for the ␣-1(I) procollagen and ␣-1(VI) collagen mRNAs, from day 6 to 14 postinduction (Fig.…”

Section: Discussionmentioning

confidence: 99%

Coordinate Expression of Novel Genes During Osteoblast Differentiation

Seth

Lee

et al. 2000

Journal of Bone and Mineral Research

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To achieve new insights into the coordinate regulation of gene expression during osteoblast differentiation we utilized an approach involving global analysis of gene expression to obtain the identities of messenger RNAs (mRNAs) expressed using an established in vitro model of bone development. MC3T3-E1 osteoblast-like cells were induced to differentiate by the addition of ␤-glycerophosphate (␤-GP) and ascorbic acid. RNA samples derived from induced and uninduced control MC3T3-E1 cells were used to prepare complementary DNA (cDNA) for serial analysis of gene expression (SAGE). A preliminary SAGE database was produced and used to prepare a hybridization array to further facilitate the characterization of changes in the expression levels of 92 of the SAGE-mRNA assignments after induction of osteoblast differentiation, specifically after 6 days and 14 days of ascorbate treatment. SAGE-array hybridization analysis revealed coordinate induction of a number of mRNAs including Rab24, calponin, and calcyclin. Levels of MSY-1, SH3P2, fibronectin, ␣-collagen, procollagen, and LAMP1 mRNAs, present at day 6 postinduction, were markedly reduced by day 14 postinduction. A number of unanticipated and potentially important developmental genes were identified including the transforming growth factor ␤ (TGF-␤) superfamily member Lefty-1. Lefty-1 transcript and translation product were found to be induced during the course of MC3T3-E1 cell differentiation. We present evidence, using

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