Search citation statements
Paper Sections
Citation Types
Year Published
Publication Types
Relationship
Authors
Journals
This paper is available on line at http://www.ejb.org/content/vol2/issue1/full/3 Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particle, forming a rattlesnake structure. dCP is the product of p27 gene for which it is also postulated a function in the transmissibility by aphid vectors. Hybridization analysis showed a p27 gene region, which exhibits different patterns with two probes derived from two biological distinct CTV isolates. In an attempt to screen whether that gene region differs in mild and severe strains, six CTV isolates belonging to different biogroups were compared for variations in their p27 gene by analysis of singlestrand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and thirty clones of each isolate were obtained. From each clone, two fragments of the gene were amplified by PCR: fragment (a), 459 bp long, and fragment (b), 281 bp long. Sequence variations in both gene fragments were studied by SSCP analysis. A variety of SSCP patterns was obtained from each isolate, being isolates belonging to the groups II-IV and III those with the higher and lower number of them. Moreover, SSCP analysis provided a rapid procedure to screen the genetic heterogeneity of the viral isolates reducing considerably the amount of nucleic acid sequenciation necessary to gain that knowledge.Citrus tristeza virus (CTV), a member of the closterovirus group is a phloem-limited virus transmitted by aphids in a semipersistent manner (Berlansky et al, 1988). The natural CTV host range is limited to large, woody plants of citrus and related genera. Because of the severe damage caused by individual citrus trees that can be productive for a century, this virus causes one of the most ecomically important diseases of plants.CTV has a positive sense RNA genome of 19,3 nucleotides (Karasev et al., 1995) encapsidated in flexuous, rod-shaped particles of about 2000 nm long (Bar-Joseph et al., 1989), with two capsid proteins: a 25 kDa coat protein (Pappu et al., 1993) covering about 95% of the particle length, and a diverged 27 kDa coat protein Febres et al., 1994) that covers one end of the particle forming a rattlesnake structure (Febres et al., 1996). Four regions of sequence conservation (domains I, II, III and IV) exist between the
This paper is available on line at http://www.ejb.org/content/vol2/issue1/full/3 Citrus tristeza closterovirus (CTV), is a phloem-limited virus transmitted by aphids in a semipersistent manner. The genome of CTV is composed of a ssRNA with two capsid proteins: CP, covering about 95% of the particle length, and a diverged coat protein (dCP), present only in one end of the particle, forming a rattlesnake structure. dCP is the product of p27 gene for which it is also postulated a function in the transmissibility by aphid vectors. Hybridization analysis showed a p27 gene region, which exhibits different patterns with two probes derived from two biological distinct CTV isolates. In an attempt to screen whether that gene region differs in mild and severe strains, six CTV isolates belonging to different biogroups were compared for variations in their p27 gene by analysis of singlestrand conformation polymorphism (SSCP). The p27 gene was reverse transcribed and amplified by PCR and thirty clones of each isolate were obtained. From each clone, two fragments of the gene were amplified by PCR: fragment (a), 459 bp long, and fragment (b), 281 bp long. Sequence variations in both gene fragments were studied by SSCP analysis. A variety of SSCP patterns was obtained from each isolate, being isolates belonging to the groups II-IV and III those with the higher and lower number of them. Moreover, SSCP analysis provided a rapid procedure to screen the genetic heterogeneity of the viral isolates reducing considerably the amount of nucleic acid sequenciation necessary to gain that knowledge.Citrus tristeza virus (CTV), a member of the closterovirus group is a phloem-limited virus transmitted by aphids in a semipersistent manner (Berlansky et al, 1988). The natural CTV host range is limited to large, woody plants of citrus and related genera. Because of the severe damage caused by individual citrus trees that can be productive for a century, this virus causes one of the most ecomically important diseases of plants.CTV has a positive sense RNA genome of 19,3 nucleotides (Karasev et al., 1995) encapsidated in flexuous, rod-shaped particles of about 2000 nm long (Bar-Joseph et al., 1989), with two capsid proteins: a 25 kDa coat protein (Pappu et al., 1993) covering about 95% of the particle length, and a diverged 27 kDa coat protein Febres et al., 1994) that covers one end of the particle forming a rattlesnake structure (Febres et al., 1996). Four regions of sequence conservation (domains I, II, III and IV) exist between the
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.