CDK5RAP2 stimulates microtubule nucleation by the γ-tubulin ring complex

Choi, Yuk Kwan; Liu, Pengfei; Sze, Siu Kwan; Dai, Chao; Qi, Robert Z.

doi:10.1083/jcb.201007030

Cited by 248 publications

(436 citation statements)

References 28 publications

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“…Finally, three proteins associated with the γ‐TuRC, Nedd1 (Haren et al , 2006; Luders et al , 2006) and Mozart 1, and Mozart 2 (Hutchins et al , 2010; Teixido‐Travesa et al , 2010) are similarly abundant as the GCP2 and GCP3 core components of γ‐TuSC. These data extend previous biochemical studies (Murphy et al , 2001; Choi et al , 2010) and support the notion that multiple γ‐TuSCs associate with GCP4, GCP5, and GCP6 into γ‐TuRCs (Kollman et al , 2011; Lin et al , 2015). …”

Section: Resultssupporting

confidence: 90%

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

et al. 2016

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Centrioles are essential for the formation of centrosomes and cilia. While numerical and/or structural centrosomes aberrations are implicated in cancer, mutations in centriolar and centrosomal proteins are genetically linked to ciliopathies, microcephaly, and dwarfism. The evolutionarily conserved mechanisms underlying centrosome biogenesis are centered on a set of key proteins, including Plk4, Sas‐6, and STIL, whose exact levels are critical to ensure accurate reproduction of centrioles during cell cycle progression. However, neither the intracellular levels of centrosomal proteins nor their stoichiometry within centrosomes is presently known. Here, we have used two complementary approaches, targeted proteomics and EGFP‐tagging of centrosomal proteins at endogenous loci, to measure protein abundance in cultured human cells and purified centrosomes. Our results provide a first assessment of the absolute and relative amounts of major components of the human centrosome. Specifically, they predict that human centriolar cartwheels comprise up to 16 stacked hubs and 1 molecule of STIL for every dimer of Sas‐6. This type of quantitative information will help guide future studies of the molecular basis of centrosome assembly and function.

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Section: Resultssupporting

confidence: 90%

Quantitative analysis of human centrosome architecture by targeted proteomics and fluorescence imaging

et al. 2016

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“…In the mouse brain, Cdk5rap2 is highly expressed in the neural progenitor pool, which accounts for the depletion of RG cells and the increase of cell cycle exit, resulting in premature neuronal differentiation (Megraw et al, 2011). Cdk5rap2 has recently been shown to stimulate microtubule nucleation (Choi et al, 2010) and regulate centriole replication (Barrera et al, 2010) via a tight link to pericentrin (Buchman et al, 2010). In mouse models, ASPM has been shown to be involved in maintaining symmetric divisions of RG cells and in completing cytokinesis (Kouprina et al, 2005;Higgins et al, 2010).…”

Section: Rg Cells and Neurodevelopmental Diseases Autosomal Recessivementioning

confidence: 99%

Neuronal stem cells in the central nervous system and in human diseases

Wang

2012

Protein Cell

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The process of cortical expansion in the central nervous system is a key step of mammalian brain development to ensure its physiological function. Radial glial (RG) cells are a glial cell type contributing to this progress as intermediate neural progenitor cells responsible for an increase in the number of cortical neurons. In this review, we discuss the current understanding of RG cells during neurogenesis and provide further information on the mechanisms of neurodevelopmental diseases and stem cell-related brain tumorigenesis. Knowledge of neuronal stem cell and relative diseases will bridge benchmark research through translational studies to clinical therapeutic treatments of these diseases.

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“…CDKRAP2 and Cep192, human counterparts of CNN and Spd-2, also increase on centrosome maturation as does pericentrin, and the proteins show interdependency for the recruitment of g-tubulin (Haren et al 2009;Lee and Rhee 2011). In part, this reflects the physical association of g-tubulin with the amino-terminal part, and pericentrin with the carboxy-terminal part of CDKRAP2 (Fong et al 2008;Choi et al 2010;Wang et al 2010).…”

Section: Pcm Assemblymentioning

confidence: 99%