CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway

Wang, Hongbin; Li, Wei; Li, Changli; Zhou, Jing; Li, Zhigao

doi:10.3892/or.2015.3736

Cited by 25 publications

(16 citation statements)

References 34 publications

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“…CDK5RAP1 also acts as an inhibitor of CDK5 to maintain healthy neuronal development and neurogenesis (37). CDK5RAP1 deficiency suppresses tumor growth and induces cell cycle arrest and apoptosis in human breast cancer (38). Although 2MeS-CKs were detected in our study (2MeSiPR, 2MeSiP, and 2MeSZR), their tissue specificity and function in mammalian physiology, beyond tRNA modification, have yet to be investigated.…”

Section: Discussionmentioning

confidence: 77%

Canis familiaris tissues are characterized by different profiles of cytokinins typical of the tRNA degradation pathway

Seegobin

Kisiała

Noble³

et al. 2018

The FASEB Journal

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Cytokinins (CKs) are a group of phytohormones essential to plant growth and development. The presence of these N-modified adenine derivatives has also been documented in other groups of organisms, including bacteria, fungi, and insects. Thus far, however, only a single CK, N-(Δ-isopentenyl) adenine-9-riboside (iPR), has been identified in mammals. In plants, the nucleotide form of isopentenyladenine [iPR (either mono-, di-, or tri-) phosphate (iPRP)] is the first form of CK synthesized, and it is further modified to produce other CK types. To determine if a similar biosynthesis pathway exists in mammals, we tested for the presence of 27 CKs in a wide selection of canine organs using HPLC electrospray ionization-tandem mass spectrometry. Seven forms of CK were detected in the majority of the analyzed samples, including iPR, iPRP, cis-zeatin-9-riboside, cis-zeatin-9-riboside-5' (either mono-, di-, or triphosphate), 2-methylthio-N-isopentenyladenine, 2-methylthio-N-isopentenyladenosine, and 2-methylthio-zeatin. Total CK concentrations ranged from 1.96 pmol/g fresh weight (adrenal glands) to 1.40 × 10 pmol/g fresh weight (thyroid). The results of this study provide evidence that mammalian cells, like plant cells, can synthesize and process a diverse set of CKs including cis- and methylthiol-type CKs.-Seegobin, M., Kisiala, A., Noble, A., Kaplan, D., Brunetti, C., Emery, R. J. N. Canis familiaris tissues are characterized by different profiles of cytokinins typical of tRNA degradation pathway.

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Section: Discussionmentioning

confidence: 77%

Canis familiaris tissues are characterized by different profiles of cytokinins typical of the tRNA degradation pathway

Seegobin

Kisiała

Noble³

et al. 2018

The FASEB Journal

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“…In the past, surgery and radiotherapy has been the most common treatment method ( 3 ). Although there is now a new era of molecular targeted therapy, these traditional therapies often bring numerous unwanted side effects and problems, and do not always improve the symptoms ( 4 ). Due to the limited efficacy traditional therapeutics, it is important to identify novel treatment strategies with reduced side effects.…”

Section: Introductionmentioning

confidence: 99%

A novel polysaccharide derived from algae extract inhibits cancer progression via JNK, not via the p38ï¿½MAPK signaling pathway

et al. 2018

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Cancer has long been one of the most malignant diseases worldwide. Processes in cancer cells are often mediated by Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (p38 MAPK) and other signaling pathways. Traditional therapies are often problematic. Recently, a novel polysaccharide derived from algae extract was investigated due to the increasing interest in biological activities of compounds from marine organisms. The effect of this novel polysaccharide on human MKN45 gastric carcinoma cells was determined previously. The current aimed to determine whether the polysaccharide affects other types of cancer, and the deeper mechanisms involved in the process. Human MCF-7 breast cancer cells were used to investigate the novel polysaccharide for its role in the cell growth and migration, and determine the mechanisms affected. MTT assay, nuclear staining and fluorescence activated cell sorting analysis demonstrated that the novel polysaccharide reduced the viability of MCF-7 cells by inducing cell apoptosis and arresting the cells at G2/M phase. Results of western blot analysis demonstrated that phosphorylation of JNK and expression of p53, caspase-9 and caspase-3 were upregulated in the polysaccharide-treated MCF-7 cells. SP600125, an inhibitor of JNK, maintained MCF-7 cell viability, prevented cell apoptosis and cycle arrest, and downregulated the polysaccharide-induced protein phosphorylation/expression. However, a migration assay demonstrated that the novel polysaccharide did not change the migration of MCF-7 cells, as well as the expression of p38 MAPK, and matrix metalloproteinase-9 and -2. Taken together, the current study demonstrated that the novel polysaccharide suppressed cancer cell growth, induced cancer cell apoptosis and cell cycle arrest via JNK signaling, but had no effect on cancer cell migration and p38 MAPK signaling.

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“…Previous studies showed that HS6ST2 was involved in ovarian cancer (Cole et al 2014) and pancreatic cancer (Song et al 2011). CDK5RAP1 was associated with tumor growth in breast cancer (Wang et al 2015). Though tubal EM is not a malignant disease, some biological behaviors of it are similar to cancer, including invasion, adhesion, angiogenesis and proliferation.…”

Section: Discussionmentioning

confidence: 97%

Genome-wide profiling of miRNA expression patterns in tubal endometriosis

Qi¹,

Guo²,

Yu³

et al. 2019

Reproduction

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MicroRNA (miRNA) expression proﬁles in tubal endometriosis (EM) are still poorly understood. In this study, we analyzed the differential expression of miRNAs and the related gene networks and signaling pathways in tubal EM. Four tubal epithelium samples from tubal EM patients and five normal tubal epithelium samples from uterine leiomyoma patients were collected for miRNA microarray. Bioinformatics analyses, including Ingenuity Pathway Analysis (IPA), Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, were performed. Quantitative real-time polymerase chain reaction (qRT-PCR) validation of five miRNAs was performed in six tubal epithelium samples from tubal EM and six from control. A total of 17 significantly differentially expressed miRNAs and 4343 potential miRNA-target genes involved in tubal EM were identified (fold change >1.5 and FDR-adjusted P value <0.05). IPA indicated connections between miRNAs, target genes and other gynecological diseases like endometrial carcinoma. GO and KEGG analysis revealed that most of the identified genes were involved in the mTOR signaling pathway, SNARE interactions in vesicular transport and endocytosis. We constructed an miRNA-gene-disease network using target gene prediction. Functional analysis showed that the mTOR pathway was connected closely to tubal EM. Our results demonstrate for the first time the differentially expressed miRNAs and the related signal pathways involved in the pathogenesis of tubal EM which contribute to elucidating the pathogenic mechanism of tubal EM-related infertility.

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CDK5RAP1 deficiency induces cell cycle arrest and apoptosis in human breast cancer cell line by the ROS/JNK signaling pathway

Cited by 25 publications

References 34 publications

Canis familiaris tissues are characterized by different profiles of cytokinins typical of the tRNA degradation pathway

Canis familiaris tissues are characterized by different profiles of cytokinins typical of the tRNA degradation pathway

A novel polysaccharide derived from algae extract inhibits cancer progression via JNK, not via the p38ï¿½MAPK signaling pathway

Genome-wide profiling of miRNA expression patterns in tubal endometriosis

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